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Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation
dc.creator | Dojnov, Biljana | |
dc.creator | Vujčić, Zoran | |
dc.date.accessioned | 2019-01-30T17:30:13Z | |
dc.date.available | 2019-01-30T17:30:13Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0003-2697 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/995 | |
dc.description.abstract | Detection of alpha-amylase and glucoamylase in crude fermentation extracts using a single native electrophoresis gel and zymogram is described in this article. Proteins were printed on substrate gel and simultaneously onto a membrane in a three-sandwich gel. alpha-Amylase was detected on the substrate gel with copolymerized beta-limit dextrins and iodine reagent. Glucoamylases were detected on the membrane using a coupled assay for glucose detection. Both amylases were detected in native gel using starch and iodine reagent. The described technique can be a helpful tool for monitoring and control of fermentation processes because fungal amylase producers almost always synthesize both amylases. | en |
dc.publisher | Academic Press Inc Elsevier Science, San Diego | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS// | |
dc.rights | restrictedAccess | |
dc.source | Analytical Biochemistry | |
dc.subject | alpha-Amylase | en |
dc.subject | Glucoamylase | en |
dc.subject | Aspergillus niger | en |
dc.subject | Zymogram | en |
dc.subject | SSF | en |
dc.title | Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Вујциц, Зоран; Дојнов, Биљана; | |
dc.citation.volume | 421 | |
dc.citation.issue | 2 | |
dc.citation.spage | 802 | |
dc.citation.epage | 804 | |
dc.citation.other | 421(2): 802-804 | |
dc.citation.rank | M22 | |
dc.identifier.pmid | 22206938 | |
dc.identifier.doi | 10.1016/j.ab.2011.11.039 | |
dc.identifier.scopus | 2-s2.0-84859722982 | |
dc.identifier.wos | 000300528400067 | |
dc.type.version | publishedVersion |