CER - Central Repository
Institute of Chemistry, Technology and Metallurgy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   CER
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
  •   CER
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation

Authorized Users Only
2012
Authors
Dojnov, Biljana
Vujčić, Zoran
Article (Published version)
Metadata
Show full item record
Abstract
Detection of alpha-amylase and glucoamylase in crude fermentation extracts using a single native electrophoresis gel and zymogram is described in this article. Proteins were printed on substrate gel and simultaneously onto a membrane in a three-sandwich gel. alpha-Amylase was detected on the substrate gel with copolymerized beta-limit dextrins and iodine reagent. Glucoamylases were detected on the membrane using a coupled assay for glucose detection. Both amylases were detected in native gel using starch and iodine reagent. The described technique can be a helpful tool for monitoring and control of fermentation processes because fungal amylase producers almost always synthesize both amylases.
Keywords:
alpha-Amylase / Glucoamylase / Aspergillus niger / Zymogram / SSF
Source:
Analytical Biochemistry, 2012, 421, 2, 802-804
Publisher:
  • Academic Press Inc Elsevier Science, San Diego
Funding / projects:
  • Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)

DOI: 10.1016/j.ab.2011.11.039

ISSN: 0003-2697

PubMed: 22206938

WoS: 000300528400067

Scopus: 2-s2.0-84859722982
[ Google Scholar ]
8
6
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/995
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Dojnov, Biljana
AU  - Vujčić, Zoran
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/995
AB  - Detection of alpha-amylase and glucoamylase in crude fermentation extracts using a single native electrophoresis gel and zymogram is described in this article. Proteins were printed on substrate gel and simultaneously onto a membrane in a three-sandwich gel. alpha-Amylase was detected on the substrate gel with copolymerized beta-limit dextrins and iodine reagent. Glucoamylases were detected on the membrane using a coupled assay for glucose detection. Both amylases were detected in native gel using starch and iodine reagent. The described technique can be a helpful tool for monitoring and control of fermentation processes because fungal amylase producers almost always synthesize both amylases.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Analytical Biochemistry
T1  - Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation
VL  - 421
IS  - 2
SP  - 802
EP  - 804
DO  - 10.1016/j.ab.2011.11.039
ER  - 
@article{
author = "Dojnov, Biljana and Vujčić, Zoran",
year = "2012",
abstract = "Detection of alpha-amylase and glucoamylase in crude fermentation extracts using a single native electrophoresis gel and zymogram is described in this article. Proteins were printed on substrate gel and simultaneously onto a membrane in a three-sandwich gel. alpha-Amylase was detected on the substrate gel with copolymerized beta-limit dextrins and iodine reagent. Glucoamylases were detected on the membrane using a coupled assay for glucose detection. Both amylases were detected in native gel using starch and iodine reagent. The described technique can be a helpful tool for monitoring and control of fermentation processes because fungal amylase producers almost always synthesize both amylases.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation",
volume = "421",
number = "2",
pages = "802-804",
doi = "10.1016/j.ab.2011.11.039"
}
Dojnov, B.,& Vujčić, Z.. (2012). Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego., 421(2), 802-804.
https://doi.org/10.1016/j.ab.2011.11.039
Dojnov B, Vujčić Z. Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation. in Analytical Biochemistry. 2012;421(2):802-804.
doi:10.1016/j.ab.2011.11.039 .
Dojnov, Biljana, Vujčić, Zoran, "Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation" in Analytical Biochemistry, 421, no. 2 (2012):802-804,
https://doi.org/10.1016/j.ab.2011.11.039 . .

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

re3dataOpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

re3dataOpenAIRERCUB