Detection of alpha-amylase and glucoamylase in crude fermentation extracts using a single native electrophoresis gel and zymogram is described in this article. Proteins were printed on substrate gel and simultaneously onto a membrane in a three-sandwich gel. alpha-Amylase was detected on the substrate gel with copolymerized beta-limit dextrins and iodine reagent. Glucoamylases were detected on the membrane using a coupled assay for glucose detection. Both amylases were detected in native gel using starch and iodine reagent. The described technique can be a helpful tool for monitoring and control of fermentation processes because fungal amylase producers almost always synthesize both amylases.
TY - JOUR
AU - Dojnov, Biljana
AU - Vujčić, Zoran
PY - 2012
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/995
AB - Detection of alpha-amylase and glucoamylase in crude fermentation extracts using a single native electrophoresis gel and zymogram is described in this article. Proteins were printed on substrate gel and simultaneously onto a membrane in a three-sandwich gel. alpha-Amylase was detected on the substrate gel with copolymerized beta-limit dextrins and iodine reagent. Glucoamylases were detected on the membrane using a coupled assay for glucose detection. Both amylases were detected in native gel using starch and iodine reagent. The described technique can be a helpful tool for monitoring and control of fermentation processes because fungal amylase producers almost always synthesize both amylases.
PB - Academic Press Inc Elsevier Science, San Diego
T2 - Analytical Biochemistry
T1 - Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation
VL - 421
IS - 2
SP - 802
EP - 804
DO - 10.1016/j.ab.2011.11.039
ER -
@article{
author = "Dojnov, Biljana and Vujčić, Zoran",
year = "2012",
abstract = "Detection of alpha-amylase and glucoamylase in crude fermentation extracts using a single native electrophoresis gel and zymogram is described in this article. Proteins were printed on substrate gel and simultaneously onto a membrane in a three-sandwich gel. alpha-Amylase was detected on the substrate gel with copolymerized beta-limit dextrins and iodine reagent. Glucoamylases were detected on the membrane using a coupled assay for glucose detection. Both amylases were detected in native gel using starch and iodine reagent. The described technique can be a helpful tool for monitoring and control of fermentation processes because fungal amylase producers almost always synthesize both amylases.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation",
volume = "421",
number = "2",
pages = "802-804",
doi = "10.1016/j.ab.2011.11.039"
}
Dojnov, B.,& Vujčić, Z.. (2012). Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego., 421(2), 802-804.
https://doi.org/10.1016/j.ab.2011.11.039
Dojnov B, Vujčić Z. Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation. in Analytical Biochemistry. 2012;421(2):802-804.
doi:10.1016/j.ab.2011.11.039 .
Dojnov, Biljana, Vujčić, Zoran, "Fast and reliable method for simultaneous zymographic detection of glucoamylase and a-amylase in fungal fermentation" in Analytical Biochemistry, 421, no. 2 (2012):802-804,
https://doi.org/10.1016/j.ab.2011.11.039 . .
