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Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy

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2012
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Authors
Milić, Jelena
Beškoski, Vladimir
Randjelović, Danijela
Stojanović, J.
Vrvić, Miroslav
Article (Published version)
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Abstract
This study visually documents the mechanical contact and interaction between the bacterial cells of two biogeocenotically different strains of Acidithiobacillus ferrooxidans (At. ferrooxidans) and oil shale containing pyrite. Atomic force microscopy (AFM) imaging was used to visualise initial interaction between the microorganisms and the surface minerals of an oil shale and to evaluate bacterial effects in the first hours of the bioleaching process. Acidithiobacillus ferrooxidans was attached to the shale surface already after 2 h, and after 48 h, numerous cells covered the surface with a biofilm. After 5 day incubation with At. ferrooxidans, AFM imaging revealed ellipsoid etched pits that represent footprints left by detached cells. Combining AFM surface imaging and leaching analysis following bacterial colonisation of oil shale layers demonstrates that an initial attachment to the surface is necessary for the leaching and that later on, once a sufficient concentration of Fe2+ ions i...n the solution is achieved, cells detach to become free cells, and leaching occurs primarily by the Fe3+. This experiment confirmed that microorganisms isolated from sites in which a particular substrate is found will demonstrate stronger binding to that substrate.

Keywords:
atomic force microscopy / Acidithiobacillus ferrooxidans / bioleaching / oil shale
Source:
Journal of Mining and Metallurgy B: Metallurgy, 2012, 48, 2, 207-217
Publisher:
  • Technical Faculty, Bor-Serbia, Bor
Funding / projects:
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)

DOI: 10.2298/JMMB110923016M

ISSN: 1450-5339

WoS: 000310129700005

Scopus: 2-s2.0-84866762460
[ Google Scholar ]
2
2
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/989
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Milić, Jelena
AU  - Beškoski, Vladimir
AU  - Randjelović, Danijela
AU  - Stojanović, J.
AU  - Vrvić, Miroslav
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/989
AB  - This study visually documents the mechanical contact and interaction between the bacterial cells of two biogeocenotically different strains of Acidithiobacillus ferrooxidans (At. ferrooxidans) and oil shale containing pyrite. Atomic force microscopy (AFM) imaging was used to visualise initial interaction between the microorganisms and the surface minerals of an oil shale and to evaluate bacterial effects in the first hours of the bioleaching process. Acidithiobacillus ferrooxidans was attached to the shale surface already after 2 h, and after 48 h, numerous cells covered the surface with a biofilm. After 5 day incubation with At. ferrooxidans, AFM imaging revealed ellipsoid etched pits that represent footprints left by detached cells. Combining AFM surface imaging and leaching analysis following bacterial colonisation of oil shale layers demonstrates that an initial attachment to the surface is necessary for the leaching and that later on, once a sufficient concentration of Fe2+ ions in the solution is achieved, cells detach to become free cells, and leaching occurs primarily by the Fe3+. This experiment confirmed that microorganisms isolated from sites in which a particular substrate is found will demonstrate stronger binding to that substrate.
PB  - Technical Faculty, Bor-Serbia, Bor
T2  - Journal of Mining and Metallurgy B: Metallurgy
T1  - Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy
VL  - 48
IS  - 2
SP  - 207
EP  - 217
DO  - 10.2298/JMMB110923016M
ER  - 
@article{
author = "Milić, Jelena and Beškoski, Vladimir and Randjelović, Danijela and Stojanović, J. and Vrvić, Miroslav",
year = "2012",
abstract = "This study visually documents the mechanical contact and interaction between the bacterial cells of two biogeocenotically different strains of Acidithiobacillus ferrooxidans (At. ferrooxidans) and oil shale containing pyrite. Atomic force microscopy (AFM) imaging was used to visualise initial interaction between the microorganisms and the surface minerals of an oil shale and to evaluate bacterial effects in the first hours of the bioleaching process. Acidithiobacillus ferrooxidans was attached to the shale surface already after 2 h, and after 48 h, numerous cells covered the surface with a biofilm. After 5 day incubation with At. ferrooxidans, AFM imaging revealed ellipsoid etched pits that represent footprints left by detached cells. Combining AFM surface imaging and leaching analysis following bacterial colonisation of oil shale layers demonstrates that an initial attachment to the surface is necessary for the leaching and that later on, once a sufficient concentration of Fe2+ ions in the solution is achieved, cells detach to become free cells, and leaching occurs primarily by the Fe3+. This experiment confirmed that microorganisms isolated from sites in which a particular substrate is found will demonstrate stronger binding to that substrate.",
publisher = "Technical Faculty, Bor-Serbia, Bor",
journal = "Journal of Mining and Metallurgy B: Metallurgy",
title = "Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy",
volume = "48",
number = "2",
pages = "207-217",
doi = "10.2298/JMMB110923016M"
}
Milić, J., Beškoski, V., Randjelović, D., Stojanović, J.,& Vrvić, M.. (2012). Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy. in Journal of Mining and Metallurgy B: Metallurgy
Technical Faculty, Bor-Serbia, Bor., 48(2), 207-217.
https://doi.org/10.2298/JMMB110923016M
Milić J, Beškoski V, Randjelović D, Stojanović J, Vrvić M. Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy. in Journal of Mining and Metallurgy B: Metallurgy. 2012;48(2):207-217.
doi:10.2298/JMMB110923016M .
Milić, Jelena, Beškoski, Vladimir, Randjelović, Danijela, Stojanović, J., Vrvić, Miroslav, "Visualisation of the interaction between Acidithiobacillus ferrooxidans and oil shale by atomic force microscopy" in Journal of Mining and Metallurgy B: Metallurgy, 48, no. 2 (2012):207-217,
https://doi.org/10.2298/JMMB110923016M . .

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