Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 - 5.0 mA cm(-2) from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping charge capacities, it was estimated that current density of 2.0 mA cm(-2) was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated.
TY - JOUR
AU - Jambrec, Daliborka
AU - Gvozdenović, Milica M.
AU - Antov, Mirjana
AU - Grgur, Branimir N.
AU - Jokić, Bojan
AU - Stevanović, Jasmina
AU - Jugović, Branimir
PY - 2012
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/975
AB - Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 - 5.0 mA cm(-2) from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping charge capacities, it was estimated that current density of 2.0 mA cm(-2) was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated.
PB - Inst Materials Physics, Bucharest
T2 - Digest Journal of Nanomaterials and Biostructures
T1 - Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose
VL - 7
IS - 2
SP - 785
EP - 794
ER -
@article{
author = "Jambrec, Daliborka and Gvozdenović, Milica M. and Antov, Mirjana and Grgur, Branimir N. and Jokić, Bojan and Stevanović, Jasmina and Jugović, Branimir",
year = "2012",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/975",
abstract = "Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 - 5.0 mA cm(-2) from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping charge capacities, it was estimated that current density of 2.0 mA cm(-2) was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated.",
publisher = "Inst Materials Physics, Bucharest",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose",
volume = "7",
number = "2",
pages = "785-794"
}
Jambrec, D., Gvozdenović, M. M., Antov, M., Grgur, B. N., Jokić, B., Stevanović, J.,& Jugović, B. (2012). Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose.
Digest Journal of Nanomaterials and Biostructures
Inst Materials Physics, Bucharest., 7(2), 785-794.
Jambrec D, Gvozdenović MM, Antov M, Grgur BN, Jokić B, Stevanović J, Jugović B. Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose. Digest Journal of Nanomaterials and Biostructures. 2012;7(2):785-794
Jambrec Daliborka, Gvozdenović Milica M., Antov Mirjana, Grgur Branimir N., Jokić Bojan, Stevanović Jasmina, Jugović Branimir, "Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose" Digest Journal of Nanomaterials and Biostructures, 7, no. 2 (2012):785-794
