Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose
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2012
Authors
Jambrec, Daliborka
Gvozdenović, Milica M.

Antov, Mirjana

Grgur, Branimir N.

Jokić, Bojan

Stevanović, Jasmina

Jugović, Branimir

Article (Published version)

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Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 - 5.0 mA cm(-2) from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping charge capacities, it was estimated that current density of 2.0 mA cm(-2) was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated.
Keywords:
Electrochemical synthesis / Polyaniline / Immobilization / Michaelis constantSource:
Digest Journal of Nanomaterials and Biostructures, 2012, 7, 2, 785-794Publisher:
- Inst Materials Physics, Bucharest
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IHTMTY - JOUR AU - Jambrec, Daliborka AU - Gvozdenović, Milica M. AU - Antov, Mirjana AU - Grgur, Branimir N. AU - Jokić, Bojan AU - Stevanović, Jasmina AU - Jugović, Branimir PY - 2012 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/975 AB - Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 - 5.0 mA cm(-2) from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping charge capacities, it was estimated that current density of 2.0 mA cm(-2) was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated. PB - Inst Materials Physics, Bucharest T2 - Digest Journal of Nanomaterials and Biostructures T1 - Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose VL - 7 IS - 2 SP - 785 EP - 794 UR - https://hdl.handle.net/21.15107/rcub_dais_469 ER -
@article{ author = "Jambrec, Daliborka and Gvozdenović, Milica M. and Antov, Mirjana and Grgur, Branimir N. and Jokić, Bojan and Stevanović, Jasmina and Jugović, Branimir", year = "2012", abstract = "Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 - 5.0 mA cm(-2) from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping charge capacities, it was estimated that current density of 2.0 mA cm(-2) was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated.", publisher = "Inst Materials Physics, Bucharest", journal = "Digest Journal of Nanomaterials and Biostructures", title = "Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose", volume = "7", number = "2", pages = "785-794", url = "https://hdl.handle.net/21.15107/rcub_dais_469" }
Jambrec, D., Gvozdenović, M. M., Antov, M., Grgur, B. N., Jokić, B., Stevanović, J.,& Jugović, B.. (2012). Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose. in Digest Journal of Nanomaterials and Biostructures Inst Materials Physics, Bucharest., 7(2), 785-794. https://hdl.handle.net/21.15107/rcub_dais_469
Jambrec D, Gvozdenović MM, Antov M, Grgur BN, Jokić B, Stevanović J, Jugović B. Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose. in Digest Journal of Nanomaterials and Biostructures. 2012;7(2):785-794. https://hdl.handle.net/21.15107/rcub_dais_469 .
Jambrec, Daliborka, Gvozdenović, Milica M., Antov, Mirjana, Grgur, Branimir N., Jokić, Bojan, Stevanović, Jasmina, Jugović, Branimir, "Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose" in Digest Journal of Nanomaterials and Biostructures, 7, no. 2 (2012):785-794, https://hdl.handle.net/21.15107/rcub_dais_469 .