Show simple item record

dc.creatorPavlovic, R.
dc.creatorGrujic, Marica
dc.creatorDojnov, Biljana
dc.creatorVujčić, Miroslava
dc.creatorNenadovic, Vera
dc.creatorIvanovic, Jelisaveta
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:29:07Z
dc.date.available2019-01-30T17:29:07Z
dc.date.issued2012
dc.identifier.issn0354-4664
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/943
dc.description.abstractThe expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven β-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and β-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-β-1,4-glucanase; subsequently, the obtained fragments are broken down by β-D-glucosidase mostly in middle midgut.en
dc.publisherInst Bioloska Istrazivanja Sinisa Stankovic, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.rightsopenAccess
dc.sourceArchives of Biological Sciences
dc.subjectCerambycidaeen
dc.subjectCerambyx cerdoen
dc.subjectcellulaseen
dc.subjectβ-D-glucosidaseen
dc.subjectendocellulaseen
dc.subjectisoformsen
dc.subjectzymogramen
dc.subjectmidguten
dc.subjectSerbiaen
dc.titleInfluence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvaeen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractВујциц, З.; Вујчић, Мирослава; Дојнов, Биљана; Павловиц, Р.; Ивановиц, Јелисавета; Ненадовиц, Вера; Грујиц, Марица;
dc.citation.volume64
dc.citation.issue2
dc.citation.spage757
dc.citation.epage765
dc.citation.other64(2): 757-765
dc.citation.rankM23
dc.identifier.doi10.2298/ABS1202757P
dc.identifier.rcubConv_2802
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/9516/941.pdf
dc.identifier.scopus2-s2.0-84862255096
dc.identifier.wos000304339100043
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record