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dc.creatorKaradžić, Ivanka
dc.creatorIzrael, L.
dc.creatorGojgić-Cvijović, Gordana
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:10:53Z
dc.date.available2019-01-30T17:10:53Z
dc.date.issued2002
dc.identifier.issn1389-1723
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/93
dc.description.abstractIn culture filtrate of Streptomyces hygroscopicus a producer of polyketide antibiotics, a leucine aminopeptidase and its autogenous inhibitor were detected. The leucine aminopeptidase was purified 4573-fold with yield of 82% by combination of ion exchange and hydrophobic chromatography. The enzyme is monomeric with a molecular mass of 51 kDa determined by gel chromatography and 67 kDa determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Optimal activity was at pH 8.0 and 40°C. The pI of leucine aminopeptidase is 8.2. The enzyme is strongly inhibited by 1,10-phenantroline, amastatin and dithiothreitol. Atomic absorption spectrometry indicated 2 mols of ion zinc per mol of enzyme. The enzyme is stable at up to 70°C. Leucine aminopeptidase prefers leucine and methionine as N-terminal amino acids. Activity of leucine aminopeptidase is strongly modulated by an autogenous low-molecular weight inhibitor during fermentation, especially during periods of intensive antibiotic production.en
dc.publisherSociety of Fermentation and Bioengineering
dc.rightsrestrictedAccess
dc.sourceJournal of Bioscience and Bioengineering
dc.subjectLeucine aminopeptidaseen
dc.subjectLow molecular weight autogenous inhibitoren
dc.subjectMetalloenzymeen
dc.subjectMonomericen
dc.subjectStreptomyces hygroscopicusen
dc.titleLeucine aminopeptidase from Streptomyces hygroscopicus is controlled by a low molecular weight inhibitoren
dc.typearticle
dc.rights.licenseARR
dcterms.abstractГојгић Цвијовић, Гордана; Карадзиц, И.; Израел, Л.; Вујциц, З.;
dc.citation.volume94
dc.citation.issue4
dc.citation.spage309
dc.citation.epage314
dc.citation.other94(4): 309-314
dc.citation.rankM22
dc.identifier.doi10.1263/jbb.94.309
dc.identifier.rcubConv_4049
dc.identifier.scopus2-s2.0-0036429142
dc.identifier.wos000180028800004
dc.type.versionpublishedVersion


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