Приказ основних података о документу
Leucine aminopeptidase from Streptomyces hygroscopicus is controlled by a low molecular weight inhibitor
dc.creator | Karadžić, Ivanka | |
dc.creator | Izrael, Lidija | |
dc.creator | Gojgić-Cvijović, Gordana | |
dc.creator | Vujčić, Zoran | |
dc.date.accessioned | 2019-01-30T17:10:53Z | |
dc.date.available | 2019-01-30T17:10:53Z | |
dc.date.issued | 2002 | |
dc.identifier.issn | 1389-1723 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/93 | |
dc.description.abstract | In culture filtrate of Streptomyces hygroscopicus a producer of polyketide antibiotics, a leucine aminopeptidase and its autogenous inhibitor were detected. The leucine aminopeptidase was purified 4573-fold with yield of 82% by combination of ion exchange and hydrophobic chromatography. The enzyme is monomeric with a molecular mass of 51 kDa determined by gel chromatography and 67 kDa determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Optimal activity was at pH 8.0 and 40°C. The pI of leucine aminopeptidase is 8.2. The enzyme is strongly inhibited by 1,10-phenantroline, amastatin and dithiothreitol. Atomic absorption spectrometry indicated 2 mols of ion zinc per mol of enzyme. The enzyme is stable at up to 70°C. Leucine aminopeptidase prefers leucine and methionine as N-terminal amino acids. Activity of leucine aminopeptidase is strongly modulated by an autogenous low-molecular weight inhibitor during fermentation, especially during periods of intensive antibiotic production. | en |
dc.publisher | Society of Fermentation and Bioengineering | |
dc.rights | restrictedAccess | |
dc.source | Journal of Bioscience and Bioengineering | |
dc.subject | Leucine aminopeptidase | en |
dc.subject | Low molecular weight autogenous inhibitor | en |
dc.subject | Metalloenzyme | en |
dc.subject | Monomeric | en |
dc.subject | Streptomyces hygroscopicus | en |
dc.title | Leucine aminopeptidase from Streptomyces hygroscopicus is controlled by a low molecular weight inhibitor | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Гојгић Цвијовић, Гордана; Карадзиц, И.; Израел, Л.; Вујциц, З.; | |
dc.citation.volume | 94 | |
dc.citation.issue | 4 | |
dc.citation.spage | 309 | |
dc.citation.epage | 314 | |
dc.citation.other | 94(4): 309-314 | |
dc.citation.rank | M22 | |
dc.identifier.doi | 10.1016/S1389-1723(02)80169-4 | |
dc.identifier.scopus | 2-s2.0-0036429142 | |
dc.identifier.wos | 000180028800004 | |
dc.type.version | publishedVersion |