Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity
Само за регистроване кориснике
2011
Аутори
Zdunić, GordanaGođevac, Dejan
Savikin, Katarina
Novaković, Miroslav
Milosavljević, Slobodan
Petrovic, Silvana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phen...olic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.
Кључне речи:
Hypericum richeri / phenolics / HPLC-DAD / on-line HPLC-DPPH / antioxidant capacityИзвор:
Natural Product Research, 2011, 25, 3, 175-187Издавач:
- Taylor & Francis Ltd, Abingdon
Финансирање / пројекти:
- Биљни производи у превентиви и терапији незаразних хроничних обољења код људи (RS-MESTD-MPN2006-2010-20035)
- Испитивање лековитог потенцијала биљака: морфолошка, хемијска и фармаколошка карактеризација (RS-MESTD-MPN2006-2010-143012)
DOI: 10.1080/14786410802401390
ISSN: 1478-6419
PubMed: 19764002
WoS: 000286894900001
Scopus: 2-s2.0-79551671472
Институција/група
IHTMTY - JOUR AU - Zdunić, Gordana AU - Gođevac, Dejan AU - Savikin, Katarina AU - Novaković, Miroslav AU - Milosavljević, Slobodan AU - Petrovic, Silvana PY - 2011 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/928 AB - The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phenolic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri. PB - Taylor & Francis Ltd, Abingdon T2 - Natural Product Research T1 - Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity VL - 25 IS - 3 SP - 175 EP - 187 DO - 10.1080/14786410802401390 ER -
@article{ author = "Zdunić, Gordana and Gođevac, Dejan and Savikin, Katarina and Novaković, Miroslav and Milosavljević, Slobodan and Petrovic, Silvana", year = "2011", abstract = "The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phenolic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Natural Product Research", title = "Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity", volume = "25", number = "3", pages = "175-187", doi = "10.1080/14786410802401390" }
Zdunić, G., Gođevac, D., Savikin, K., Novaković, M., Milosavljević, S.,& Petrovic, S.. (2011). Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity. in Natural Product Research Taylor & Francis Ltd, Abingdon., 25(3), 175-187. https://doi.org/10.1080/14786410802401390
Zdunić G, Gođevac D, Savikin K, Novaković M, Milosavljević S, Petrovic S. Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity. in Natural Product Research. 2011;25(3):175-187. doi:10.1080/14786410802401390 .
Zdunić, Gordana, Gođevac, Dejan, Savikin, Katarina, Novaković, Miroslav, Milosavljević, Slobodan, Petrovic, Silvana, "Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity" in Natural Product Research, 25, no. 3 (2011):175-187, https://doi.org/10.1080/14786410802401390 . .