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Streptomyces subtilizinu sličan inhibitor (SSL-inhibitor) iz streptomyces hygroscopicus CH-7 - detekcija i delimična karakterizacija

dc.creatorIzrael, Lidija
dc.creatorKaradžić, Ivanka
dc.creatorGojgić-Cvijović, Gordana
dc.creatorVrvić, Miroslav
dc.date.accessioned2019-01-30T17:08:58Z
dc.date.available2019-01-30T17:08:58Z
dc.date.issued2000
dc.identifier.issn0581-1538
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/8
dc.description.abstractStrain Streptomyces (S.) hygroscopicus CH-7 during the fermen­tation of polyketide antibiotic produces as a by product proteolytic com­plex with significant trypsin like activity. Also, S. hygroscopicus CH-7 produces extracellulary proteinaceous protease inhibitor known as Streptomyces subtilisine like inhibitor (SSL-inhibitor). Detection of SSL-inhibitor is preformed on the base of stability of SSL-inhibitors on very low pH (final concentracion of TCA is 9%). Molecular mass of SSL-inhibitor is approximately 11 kDa, and it provides very wide range of inhibitory activity toward different serin proteases: trypsin, protease from Bacillus (B.) subtilis, proteinase K from Tritirachium (T.) album, pro­tease from B. licheniformis, protease from S. griseus and the complex of proteases from S. hygroscopicus CH-7.en
dc.description.abstractSoj S. hygroscopicus CH-7 u toku fermentacije ekstracelularno proizvodi proteinski inhibitor proteaza koji pripada grupi Streptomyces subtilizinu sličnim inhibitorima (Streptomyces subtilisine like inhibitor: SSL-inhibitor). Postupak za detekciju je jednostavan i bazira se na činjenici da su SSL-inhibitori stabilni na veoma niskim pH. SSL-inhibitor iz S. hygroscopicus CH-7 pokazuje širok spektar inhibitornog dejstva na: tripsin, proteazu iz B. subtilis, proteinazu K iz T. album, proteazu iz B. licheniformis, proteazu iz S. griseus i na sirovi proteoliticki kompleks S. hygroscopicus CH-7. Molekulska masa SSL-inhibitora određena gel filtracijom iznosi 11 kDa, slično većini do sada izolovanih SSL.sr
dc.rightsopenAccess
dc.sourceActa biologica iugoslavica - serija B: Mikrobiologija
dc.subjectSSL-inhibitoren
dc.subjectStrepromyces hygroscopicus CH-7en
dc.subjectproductionen
dc.subjectdetectionen
dc.subjectpartial characterizationen
dc.titleStreptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterizationen
dc.titleStreptomyces subtilizinu sličan inhibitor (SSL-inhibitor) iz streptomyces hygroscopicus CH-7 - detekcija i delimična karakterizacijasr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractИзраел, Лидија; Гојгић Цвијовић, Гордана; Врвић, Мирослав М.; Караджић, Иванка;
dc.citation.volume37
dc.citation.issue1
dc.citation.spage31
dc.citation.epage36
dc.citation.other37(1): 31-36
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_cer_8
dc.identifier.rcubConv_480
dc.type.versionpublishedVersion


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