Highly efficient raw starch digesting alpha-amylase was produced after 24 h of batch fermentation of Bacillus licheniformis ATCC 9945a in laboratory bioreactor at 37 degrees C. The enzyme was purified by gel filtration chromatographies with 6-fold increase of specific activity and 38% recovery and showed a molecular mass of 31 kDa by SDS-PAGE. The purified enzyme had an optimum pH of 6.5 and optimum temperature of 90 degrees C. The purified alpha-amylase in the presence of CaCl(2) retained 55% of its activity after 6 h of incubation at 70 degrees C. Co(2+), Ni(2+) and Ca(2+) slightly stimulated, while Hg(2+) completely inhibited alpha-amylase activity. Hydrolysis rates of raw triticale, wheat, potato, horseradish and corn starches, at 1% concentration were 63, 60, 59, 52 and 37%, respectively, in a period of 4 h. The properties of the purified enzyme proved its high efficacy for digesting diverse raw starches below gelatinization temperature and, hence, its potential commercial value t...o use as an industrial enzyme.
TY - JOUR
AU - Božić, Nataša
AU - Ruiz, Jordi
AU - Lopez-Santin, Josep
AU - Vujčić, Zoran
PY - 2011
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/899
AB - Highly efficient raw starch digesting alpha-amylase was produced after 24 h of batch fermentation of Bacillus licheniformis ATCC 9945a in laboratory bioreactor at 37 degrees C. The enzyme was purified by gel filtration chromatographies with 6-fold increase of specific activity and 38% recovery and showed a molecular mass of 31 kDa by SDS-PAGE. The purified enzyme had an optimum pH of 6.5 and optimum temperature of 90 degrees C. The purified alpha-amylase in the presence of CaCl(2) retained 55% of its activity after 6 h of incubation at 70 degrees C. Co(2+), Ni(2+) and Ca(2+) slightly stimulated, while Hg(2+) completely inhibited alpha-amylase activity. Hydrolysis rates of raw triticale, wheat, potato, horseradish and corn starches, at 1% concentration were 63, 60, 59, 52 and 37%, respectively, in a period of 4 h. The properties of the purified enzyme proved its high efficacy for digesting diverse raw starches below gelatinization temperature and, hence, its potential commercial value to use as an industrial enzyme.
PB - Elsevier Science Sa, Lausanne
T2 - Biochemical Engineering Journal
T1 - Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a
VL - 53
IS - 2
SP - 203
EP - 209
DO - 10.1016/j.bej.2010.10.014
ER -
@article{
author = "Božić, Nataša and Ruiz, Jordi and Lopez-Santin, Josep and Vujčić, Zoran",
year = "2011",
abstract = "Highly efficient raw starch digesting alpha-amylase was produced after 24 h of batch fermentation of Bacillus licheniformis ATCC 9945a in laboratory bioreactor at 37 degrees C. The enzyme was purified by gel filtration chromatographies with 6-fold increase of specific activity and 38% recovery and showed a molecular mass of 31 kDa by SDS-PAGE. The purified enzyme had an optimum pH of 6.5 and optimum temperature of 90 degrees C. The purified alpha-amylase in the presence of CaCl(2) retained 55% of its activity after 6 h of incubation at 70 degrees C. Co(2+), Ni(2+) and Ca(2+) slightly stimulated, while Hg(2+) completely inhibited alpha-amylase activity. Hydrolysis rates of raw triticale, wheat, potato, horseradish and corn starches, at 1% concentration were 63, 60, 59, 52 and 37%, respectively, in a period of 4 h. The properties of the purified enzyme proved its high efficacy for digesting diverse raw starches below gelatinization temperature and, hence, its potential commercial value to use as an industrial enzyme.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Biochemical Engineering Journal",
title = "Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a",
volume = "53",
number = "2",
pages = "203-209",
doi = "10.1016/j.bej.2010.10.014"
}
Božić, N., Ruiz, J., Lopez-Santin, J.,& Vujčić, Z.. (2011). Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a. in Biochemical Engineering Journal
Elsevier Science Sa, Lausanne., 53(2), 203-209.
https://doi.org/10.1016/j.bej.2010.10.014
Božić N, Ruiz J, Lopez-Santin J, Vujčić Z. Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a. in Biochemical Engineering Journal. 2011;53(2):203-209.
doi:10.1016/j.bej.2010.10.014 .
Božić, Nataša, Ruiz, Jordi, Lopez-Santin, Josep, Vujčić, Zoran, "Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a" in Biochemical Engineering Journal, 53, no. 2 (2011):203-209,
https://doi.org/10.1016/j.bej.2010.10.014 . .
