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Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a

Authorized Users Only
2011
Authors
Božić, Nataša
Ruiz, Jordi
Lopez-Santin, Josep
Vujčić, Zoran
Article (Published version)
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Abstract
Highly efficient raw starch digesting alpha-amylase was produced after 24 h of batch fermentation of Bacillus licheniformis ATCC 9945a in laboratory bioreactor at 37 degrees C. The enzyme was purified by gel filtration chromatographies with 6-fold increase of specific activity and 38% recovery and showed a molecular mass of 31 kDa by SDS-PAGE. The purified enzyme had an optimum pH of 6.5 and optimum temperature of 90 degrees C. The purified alpha-amylase in the presence of CaCl(2) retained 55% of its activity after 6 h of incubation at 70 degrees C. Co(2+), Ni(2+) and Ca(2+) slightly stimulated, while Hg(2+) completely inhibited alpha-amylase activity. Hydrolysis rates of raw triticale, wheat, potato, horseradish and corn starches, at 1% concentration were 63, 60, 59, 52 and 37%, respectively, in a period of 4 h. The properties of the purified enzyme proved its high efficacy for digesting diverse raw starches below gelatinization temperature and, hence, its potential commercial value t...o use as an industrial enzyme.

Keywords:
alpha-Amylase / Bacillus licheniformis / Raw starch hydrolysis / Thermostable / Purification
Source:
Biochemical Engineering Journal, 2011, 53, 2, 203-209
Publisher:
  • Elsevier Science Sa, Lausanne
Funding / projects:
  • Interakcije prirodnih proizvoda i njihovih analoga sa proteinima i nukleinskim kiselinama (RS-142026)
  • Spanish MICINN [CTQ2008-00578]

DOI: 10.1016/j.bej.2010.10.014

ISSN: 1369-703X

WoS: 000287116300007

Scopus: 2-s2.0-78649966240
[ Google Scholar ]
83
69
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/899
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Božić, Nataša
AU  - Ruiz, Jordi
AU  - Lopez-Santin, Josep
AU  - Vujčić, Zoran
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/899
AB  - Highly efficient raw starch digesting alpha-amylase was produced after 24 h of batch fermentation of Bacillus licheniformis ATCC 9945a in laboratory bioreactor at 37 degrees C. The enzyme was purified by gel filtration chromatographies with 6-fold increase of specific activity and 38% recovery and showed a molecular mass of 31 kDa by SDS-PAGE. The purified enzyme had an optimum pH of 6.5 and optimum temperature of 90 degrees C. The purified alpha-amylase in the presence of CaCl(2) retained 55% of its activity after 6 h of incubation at 70 degrees C. Co(2+), Ni(2+) and Ca(2+) slightly stimulated, while Hg(2+) completely inhibited alpha-amylase activity. Hydrolysis rates of raw triticale, wheat, potato, horseradish and corn starches, at 1% concentration were 63, 60, 59, 52 and 37%, respectively, in a period of 4 h. The properties of the purified enzyme proved its high efficacy for digesting diverse raw starches below gelatinization temperature and, hence, its potential commercial value to use as an industrial enzyme.
PB  - Elsevier Science Sa, Lausanne
T2  - Biochemical Engineering Journal
T1  - Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a
VL  - 53
IS  - 2
SP  - 203
EP  - 209
DO  - 10.1016/j.bej.2010.10.014
ER  - 
@article{
author = "Božić, Nataša and Ruiz, Jordi and Lopez-Santin, Josep and Vujčić, Zoran",
year = "2011",
abstract = "Highly efficient raw starch digesting alpha-amylase was produced after 24 h of batch fermentation of Bacillus licheniformis ATCC 9945a in laboratory bioreactor at 37 degrees C. The enzyme was purified by gel filtration chromatographies with 6-fold increase of specific activity and 38% recovery and showed a molecular mass of 31 kDa by SDS-PAGE. The purified enzyme had an optimum pH of 6.5 and optimum temperature of 90 degrees C. The purified alpha-amylase in the presence of CaCl(2) retained 55% of its activity after 6 h of incubation at 70 degrees C. Co(2+), Ni(2+) and Ca(2+) slightly stimulated, while Hg(2+) completely inhibited alpha-amylase activity. Hydrolysis rates of raw triticale, wheat, potato, horseradish and corn starches, at 1% concentration were 63, 60, 59, 52 and 37%, respectively, in a period of 4 h. The properties of the purified enzyme proved its high efficacy for digesting diverse raw starches below gelatinization temperature and, hence, its potential commercial value to use as an industrial enzyme.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Biochemical Engineering Journal",
title = "Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a",
volume = "53",
number = "2",
pages = "203-209",
doi = "10.1016/j.bej.2010.10.014"
}
Božić, N., Ruiz, J., Lopez-Santin, J.,& Vujčić, Z.. (2011). Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a. in Biochemical Engineering Journal
Elsevier Science Sa, Lausanne., 53(2), 203-209.
https://doi.org/10.1016/j.bej.2010.10.014
Božić N, Ruiz J, Lopez-Santin J, Vujčić Z. Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a. in Biochemical Engineering Journal. 2011;53(2):203-209.
doi:10.1016/j.bej.2010.10.014 .
Božić, Nataša, Ruiz, Jordi, Lopez-Santin, Josep, Vujčić, Zoran, "Production and properties of the highly efficient raw starch digesting alpha-amylase from a Bacillus licheniformis ATCC 9945a" in Biochemical Engineering Journal, 53, no. 2 (2011):203-209,
https://doi.org/10.1016/j.bej.2010.10.014 . .

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