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dc.creatorKozarski, Maja S.
dc.creatorKlaus, Anita
dc.creatorNikšić, Miomir
dc.creatorJakovljević, Dragica
dc.creatorHelsper, Johannes P. F. G.
dc.creatorVan Griensven, Leo J. L. D.
dc.date.accessioned2019-01-30T17:27:17Z
dc.date.available2019-01-30T17:27:17Z
dc.date.issued2011
dc.identifier.issn0308-8146
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/856
dc.description.abstractPartially purified polysaccharides were obtained from four medicinal mushroom species, Agaricus bisporus, Agaricus brasiliensis, Phellinus linteus and Ganoderma lucidum by hot water extraction, followed by ethanol precipitation. The four samples contained varying amounts of both alpha- and beta-glucans as determined by FT-IR and by quantitative estimation after prior partial hydrolysis (Megazyme beta-glucan assay kit). EC50 values of the DPPH scavenging activity of the polysaccharides from G. lucidum spores and P. linteus fruiting bodies were found to be particularly low, i.e. EC50 LT 0.1 mg/ml. For A. brasiliensis and A. bisporus, EC50 values were 0.27 and 2.0 mg/ml. EC50 values of the antioxidant activity were 7.07 mg/ml for G. lucidum, 13.25 mg/ml for A. brasiliensis and >20 mg/ml for A. bisporus polysaccharide, respectively. EC50 values of the chelating activity of ferrous ions ranged from 0.59 mg/ml for G. lucidum to 7.80 mg/ml for A. bisporus. The EC50 values of the extracts in the reducing power assay ranged from 0.47 to 14.83 mg/ml. A correlation was found between EC50 values of the chelating and reducing power abilities and the amount of total glucans content in the extracts. In vitro measurements of immunomodulatory capacity of polysaccharide extracts showed that A. bisporus, A. brasiliensis fruiting bodies and G. lucidum spores extracts express an immunostimulating effect on activated human PBMCs and induce synthesis of IFN-gamma. The polysaccharide extract of P. linteus fruiting bodies showed an immunosuppressive effect.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationFederation of European Microbiological Societies (FEMS)
dc.relationSerbian Ministry of Science and Technological Development
dc.rightsrestrictedAccess
dc.sourceFood Chemistry
dc.subjectAntioxidant activityen
dc.subjectImmunomodulating activityen
dc.subjectMedicinal mushroomsen
dc.subjectPolysaccharidesen
dc.titleAntioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteusen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКлаус, Aнита; Козарски, Маја; Никсиц, Миомир; Ван, Гриенсвен Лео Ј. Л. Д.; Хелспер, Јоханнес П. Ф. Г.; Јаковљевић, Драгица;
dc.citation.volume129
dc.citation.issue4
dc.citation.spage1667
dc.citation.epage1675
dc.citation.other129(4): 1667-1675
dc.citation.rankaM21
dc.identifier.doi10.1016/j.foodchem.2011.06.029
dc.identifier.rcubConv_2658
dc.identifier.scopus2-s2.0-80051789230
dc.identifier.wos000294979600047
dc.type.versionpublishedVersion


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