Effect of Candida antarctica Lipase B Immobilization on the Porous Structure of the Carrier
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A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-fold) and the specific volume (up to 2.1-fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde-modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized lipase are studied.
Keywords:diffusion / enzymes / immobilization / lipase / macroporous copolymers
Source:Macromolecular Bioscience, 2011, 11, 11, 1537-1543
- Wiley-V C H Verlag Gmbh, Weinheim