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Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization

Streptomyces subtilizinu sličan inhibitor (SSL-inhibitor) iz streptomyces hygroscopicus CH-7 - detekcija i delimična karakterizacija

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Authors
Izrael, Lidija
Karadžić, Ivanka
Gojgić-Cvijović, Gordana
Vrvić, Miroslav
Article (Published version)
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Abstract
Strain Streptomyces (S.) hygroscopicus CH-7 during the fermen­tation of polyketide antibiotic produces as a by product proteolytic com­plex with significant trypsin like activity. Also, S. hygroscopicus CH-7 produces extracellulary proteinaceous protease inhibitor known as Streptomyces subtilisine like inhibitor (SSL-inhibitor). Detection of SSL-inhibitor is preformed on the base of stability of SSL-inhibitors on very low pH (final concentracion of TCA is 9%). Molecular mass of SSL-inhibitor is approximately 11 kDa, and it provides very wide range of inhibitory activity toward different serin proteases: trypsin, protease from Bacillus (B.) subtilis, proteinase K from Tritirachium (T.) album, pro­tease from B. licheniformis, protease from S. griseus and the complex of proteases from S. hygroscopicus CH-7.
Soj S. hygroscopicus CH-7 u toku fermentacije ekstracelularno proizvodi proteinski inhibitor proteaza koji pripada grupi Streptomyces subtilizinu sličnim inhibitorima (Streptomyces subtilisine like inhibitor: SSL-inhibitor). Postupak za detekciju je jednostavan i bazira se na činjenici da su SSL-inhibitori stabilni na veoma niskim pH. SSL-inhibitor iz S. hygroscopicus CH-7 pokazuje širok spektar inhibitornog dejstva na: tripsin, proteazu iz B. subtilis, proteinazu K iz T. album, proteazu iz B. licheniformis, proteazu iz S. griseus i na sirovi proteoliticki kompleks S. hygroscopicus CH-7. Molekulska masa SSL-inhibitora određena gel filtracijom iznosi 11 kDa, slično većini do sada izolovanih SSL.
Keywords:
SSL-inhibitor / Strepromyces hygroscopicus CH-7 / production / detection / partial characterization
Source:
Acta biologica iugoslavica - serija B: Mikrobiologija, 2000, 37, 1, 31-36

ISSN: 0581-1538

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_cer_8
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/8
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Izrael, Lidija
AU  - Karadžić, Ivanka
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
PY  - 2000
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/8
AB  - Strain Streptomyces (S.) hygroscopicus CH-7 during the fermen­tation of polyketide antibiotic produces as a by product proteolytic com­plex with significant trypsin like activity. Also, S. hygroscopicus CH-7 produces extracellulary proteinaceous protease inhibitor known as Streptomyces subtilisine like inhibitor (SSL-inhibitor). Detection of SSL-inhibitor is preformed on the base of stability of SSL-inhibitors on very low pH (final concentracion of TCA is 9%). Molecular mass of SSL-inhibitor is approximately 11 kDa, and it provides very wide range of inhibitory activity toward different serin proteases: trypsin, protease from Bacillus (B.) subtilis, proteinase K from Tritirachium (T.) album, pro­tease from B. licheniformis, protease from S. griseus and the complex of proteases from S. hygroscopicus CH-7.
AB  - Soj S. hygroscopicus CH-7 u toku fermentacije ekstracelularno proizvodi proteinski inhibitor proteaza koji pripada grupi Streptomyces subtilizinu sličnim inhibitorima (Streptomyces subtilisine like inhibitor: SSL-inhibitor). Postupak za detekciju je jednostavan i bazira se na činjenici da su SSL-inhibitori stabilni na veoma niskim pH. SSL-inhibitor iz S. hygroscopicus CH-7 pokazuje širok spektar inhibitornog dejstva na: tripsin, proteazu iz B. subtilis, proteinazu K iz T. album, proteazu iz B. licheniformis, proteazu iz S. griseus i na sirovi proteoliticki kompleks S. hygroscopicus CH-7. Molekulska masa SSL-inhibitora određena gel filtracijom iznosi 11 kDa, slično većini do sada izolovanih SSL.
T2  - Acta biologica iugoslavica - serija B: Mikrobiologija
T1  - Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization
T1  - Streptomyces subtilizinu sličan inhibitor (SSL-inhibitor) iz streptomyces hygroscopicus CH-7 - detekcija i delimična karakterizacija
VL  - 37
IS  - 1
SP  - 31
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_cer_8
ER  - 
@article{
author = "Izrael, Lidija and Karadžić, Ivanka and Gojgić-Cvijović, Gordana and Vrvić, Miroslav",
year = "2000",
abstract = "Strain Streptomyces (S.) hygroscopicus CH-7 during the fermen­tation of polyketide antibiotic produces as a by product proteolytic com­plex with significant trypsin like activity. Also, S. hygroscopicus CH-7 produces extracellulary proteinaceous protease inhibitor known as Streptomyces subtilisine like inhibitor (SSL-inhibitor). Detection of SSL-inhibitor is preformed on the base of stability of SSL-inhibitors on very low pH (final concentracion of TCA is 9%). Molecular mass of SSL-inhibitor is approximately 11 kDa, and it provides very wide range of inhibitory activity toward different serin proteases: trypsin, protease from Bacillus (B.) subtilis, proteinase K from Tritirachium (T.) album, pro­tease from B. licheniformis, protease from S. griseus and the complex of proteases from S. hygroscopicus CH-7., Soj S. hygroscopicus CH-7 u toku fermentacije ekstracelularno proizvodi proteinski inhibitor proteaza koji pripada grupi Streptomyces subtilizinu sličnim inhibitorima (Streptomyces subtilisine like inhibitor: SSL-inhibitor). Postupak za detekciju je jednostavan i bazira se na činjenici da su SSL-inhibitori stabilni na veoma niskim pH. SSL-inhibitor iz S. hygroscopicus CH-7 pokazuje širok spektar inhibitornog dejstva na: tripsin, proteazu iz B. subtilis, proteinazu K iz T. album, proteazu iz B. licheniformis, proteazu iz S. griseus i na sirovi proteoliticki kompleks S. hygroscopicus CH-7. Molekulska masa SSL-inhibitora određena gel filtracijom iznosi 11 kDa, slično većini do sada izolovanih SSL.",
journal = "Acta biologica iugoslavica - serija B: Mikrobiologija",
title = "Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization, Streptomyces subtilizinu sličan inhibitor (SSL-inhibitor) iz streptomyces hygroscopicus CH-7 - detekcija i delimična karakterizacija",
volume = "37",
number = "1",
pages = "31-36",
url = "https://hdl.handle.net/21.15107/rcub_cer_8"
}
Izrael, L., Karadžić, I., Gojgić-Cvijović, G.,& Vrvić, M.. (2000). Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization. in Acta biologica iugoslavica - serija B: Mikrobiologija, 37(1), 31-36.
https://hdl.handle.net/21.15107/rcub_cer_8
Izrael L, Karadžić I, Gojgić-Cvijović G, Vrvić M. Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization. in Acta biologica iugoslavica - serija B: Mikrobiologija. 2000;37(1):31-36.
https://hdl.handle.net/21.15107/rcub_cer_8 .
Izrael, Lidija, Karadžić, Ivanka, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, "Streptomyces subtilisine (SSL) inhibitor from streptomyces hygroscopius CH-7: Detection and partial characterization" in Acta biologica iugoslavica - serija B: Mikrobiologija, 37, no. 1 (2000):31-36,
https://hdl.handle.net/21.15107/rcub_cer_8 .

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