Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions
Само за регистроване кориснике
2011
Аутори
Bajic, AleksandarZakrzewska, Joanna
Gođevac, Dejan
Anđus, Pavle
Jones, David R.
Spasić, Mihajlo
Spasojević, Ivan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not ...sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.
Кључне речи:
Fructose 1,6-bis(phosphate) / Chelation / Iron / Cerebrospinal fluid / EPR / P-31 NMRИзвор:
Carbohydrate Research, 2011, 346, 3, 416-420Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Молекуларни механизми редокс сигналинга у хомеостази, адаптацији и патологији (RS-MESTD-Basic Research (BR or ON)-173014)
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-MESTD-Basic Research (BR or ON)-173040)
DOI: 10.1016/j.carres.2010.12.008
ISSN: 0008-6215
PubMed: 21232735
WoS: 000293037400005
Scopus: 2-s2.0-79951510026
Институција/група
IHTMTY - JOUR AU - Bajic, Aleksandar AU - Zakrzewska, Joanna AU - Gođevac, Dejan AU - Anđus, Pavle AU - Jones, David R. AU - Spasić, Mihajlo AU - Spasojević, Ivan PY - 2011 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/781 AB - The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits. PB - Elsevier Sci Ltd, Oxford T2 - Carbohydrate Research T1 - Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions VL - 346 IS - 3 SP - 416 EP - 420 DO - 10.1016/j.carres.2010.12.008 ER -
@article{ author = "Bajic, Aleksandar and Zakrzewska, Joanna and Gođevac, Dejan and Anđus, Pavle and Jones, David R. and Spasić, Mihajlo and Spasojević, Ivan", year = "2011", abstract = "The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Carbohydrate Research", title = "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions", volume = "346", number = "3", pages = "416-420", doi = "10.1016/j.carres.2010.12.008" }
Bajic, A., Zakrzewska, J., Gođevac, D., Anđus, P., Jones, D. R., Spasić, M.,& Spasojević, I.. (2011). Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research Elsevier Sci Ltd, Oxford., 346(3), 416-420. https://doi.org/10.1016/j.carres.2010.12.008
Bajic A, Zakrzewska J, Gođevac D, Anđus P, Jones DR, Spasić M, Spasojević I. Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research. 2011;346(3):416-420. doi:10.1016/j.carres.2010.12.008 .
Bajic, Aleksandar, Zakrzewska, Joanna, Gođevac, Dejan, Anđus, Pavle, Jones, David R., Spasić, Mihajlo, Spasojević, Ivan, "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions" in Carbohydrate Research, 346, no. 3 (2011):416-420, https://doi.org/10.1016/j.carres.2010.12.008 . .