Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions
Authorized Users Only
2011
Authors
Bajic, Aleksandar
Zakrzewska, Joanna
Gođevac, Dejan

Anđus, Pavle
Jones, David R.
Spasić, Mihajlo

Spasojević, Ivan

Article (Published version)

Metadata
Show full item recordAbstract
The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not ...sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.
Keywords:
Fructose 1,6-bis(phosphate) / Chelation / Iron / Cerebrospinal fluid / EPR / P-31 NMRSource:
Carbohydrate Research, 2011, 346, 3, 416-420Publisher:
- Elsevier Sci Ltd, Oxford
Funding / projects:
- Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology (RS-173014)
- The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (RS-173040)
DOI: 10.1016/j.carres.2010.12.008
ISSN: 0008-6215
PubMed: 21232735
WoS: 000293037400005
Scopus: 2-s2.0-79951510026
Collections
Institution/Community
IHTMTY - JOUR AU - Bajic, Aleksandar AU - Zakrzewska, Joanna AU - Gođevac, Dejan AU - Anđus, Pavle AU - Jones, David R. AU - Spasić, Mihajlo AU - Spasojević, Ivan PY - 2011 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/781 AB - The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits. PB - Elsevier Sci Ltd, Oxford T2 - Carbohydrate Research T1 - Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions VL - 346 IS - 3 SP - 416 EP - 420 DO - 10.1016/j.carres.2010.12.008 ER -
@article{ author = "Bajic, Aleksandar and Zakrzewska, Joanna and Gođevac, Dejan and Anđus, Pavle and Jones, David R. and Spasić, Mihajlo and Spasojević, Ivan", year = "2011", abstract = "The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Carbohydrate Research", title = "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions", volume = "346", number = "3", pages = "416-420", doi = "10.1016/j.carres.2010.12.008" }
Bajic, A., Zakrzewska, J., Gođevac, D., Anđus, P., Jones, D. R., Spasić, M.,& Spasojević, I.. (2011). Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research Elsevier Sci Ltd, Oxford., 346(3), 416-420. https://doi.org/10.1016/j.carres.2010.12.008
Bajic A, Zakrzewska J, Gođevac D, Anđus P, Jones DR, Spasić M, Spasojević I. Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research. 2011;346(3):416-420. doi:10.1016/j.carres.2010.12.008 .
Bajic, Aleksandar, Zakrzewska, Joanna, Gođevac, Dejan, Anđus, Pavle, Jones, David R., Spasić, Mihajlo, Spasojević, Ivan, "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions" in Carbohydrate Research, 346, no. 3 (2011):416-420, https://doi.org/10.1016/j.carres.2010.12.008 . .