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Evaluation of the thermal stability and digestibility of heterologously produced banana lectin

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2010
Authors
Dimitrijević, Rajna
Jadranin, Milka
Burazer, Lidija
Ostojić, Sanja
Gavrović-Jankulović, Marija
Article (Published version)
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Abstract
The thermal stability of recombinant mannose-specific banana lectin (rBanLec), as well as its stability under conditions of simulated gastro-intestinal fluid (SGF), was investigated. rBanLec was heterologously produced in Escherichia coli, Molecular mass of rBanLec, assessed by ESI-TOF mass spectrometry, was 15972.2 Da. Thermodynamic parameters for rBanLec denaturation, obtained by differential scanning calorimetry (DSC), revealed a transition maximum temperature (T-m) of 60.8 degrees C, calorimetric enthalpy (H-cal) of 136.17 kcal/mol and van't Hoff enthalpy (H-VH) of 50.27 kcal/mol. rBanLec was stable following an incubation for 2 h in SGF, and then for I h, in the simulated intestinal fluid (SIF). Intact primary structure, biological and immunological reactivity of rBanLec were all preserved following treatment under SGF and SIF conditions. In conclusion, rBanLec is a good candidate for the novel bioadhesive lectin-based drug delivery systems to the gastro-intestinal tract (GIT).
Keywords:
Recombinant banana lectin / DSC / Digestion stability
Source:
Food Chemistry, 2010, 120, 4, 1113-1118
Publisher:
  • Elsevier Sci Ltd, Oxford
Projects:
  • Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima (RS-142020)

DOI: 10.1016/j.foodchem.2009.11.062

ISSN: 0308-8146

WoS: 000275010600022

Scopus: 2-s2.0-75049084167
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/743
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IHTM

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