dc.creator | Šunderić, Miloš | |
dc.creator | Gligorijević, Nikola | |
dc.creator | Minić, Simeon | |
dc.creator | Nedić, Olgica | |
dc.creator | Nikolić, Milan | |
dc.date.accessioned | 2024-01-04T17:54:33Z | |
dc.date.available | 2024-01-04T17:54:33Z | |
dc.date.issued | 2021 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/7289 | |
dc.description.abstract | In this study, the interaction between phycocyanobilin (PCB)1, a bioactive chromophore of
blue-green algae Spirulina's phycobiliproteins, and alpha-2-macroglobulin (α2M)2, a
universal anti-proteinase, was investigated under simulated physiological conditions using
spectroscopic techniques and α2M activity assay. Using spectrofluorimetric measurements,
we found that α2M binds PCB with a moderate affinity, with a binding constant of 6.3×
105 M−1 at 25°C. The binding of PCB to α2M does not cause any significant change in the
secondary structure of the protein (circular dichroism measurements). Besides, PCB
protects α2M from structural oxidative alterations under AAPH-induced free radical
overproduction. Further, PCB binding effectively preserves α2M anti-proteinase activity.
Since α2M is involved in controlling the action of enzymes during the inflammatory
process, the protection that PCB expresses could indirectly influence the intensity and
direction of body response to impaired homeostasis, especially under oxidative stress. | sr |
dc.language.iso | en | sr |
dc.publisher | Serbian Biochemical Society | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200019/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia | sr |
dc.subject | phycocyanobilin | sr |
dc.subject | alpha-2-macroglobulin | sr |
dc.subject | binding | sr |
dc.title | Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications | sr |
dc.type | conferenceObject | sr |
dc.rights.license | BY | sr |
dc.citation.spage | 163 | |
dc.citation.epage | 163 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_cer_7289 | |
dc.identifier.fulltext | http://cer.ihtm.bg.ac.rs/bitstream/id/27298/2021-Konferencija-BDS.pdf | |
dc.type.version | publishedVersion | sr |