Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications
Апстракт
In this study, the interaction between phycocyanobilin (PCB)1, a bioactive chromophore of
blue-green algae Spirulina's phycobiliproteins, and alpha-2-macroglobulin (α2M)2, a
universal anti-proteinase, was investigated under simulated physiological conditions using
spectroscopic techniques and α2M activity assay. Using spectrofluorimetric measurements,
we found that α2M binds PCB with a moderate affinity, with a binding constant of 6.3×
105 M−1 at 25°C. The binding of PCB to α2M does not cause any significant change in the
secondary structure of the protein (circular dichroism measurements). Besides, PCB
protects α2M from structural oxidative alterations under AAPH-induced free radical
overproduction. Further, PCB binding effectively preserves α2M anti-proteinase activity.
Since α2M is involved in controlling the action of enzymes during the inflammatory
process, the protection that PCB expresses could indirectly influence the intensity and
direction of body response to impai...red homeostasis, especially under oxidative stress.
Кључне речи:
phycocyanobilin / alpha-2-macroglobulin / bindingИзвор:
Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia, 2021, 163-163Издавач:
- Serbian Biochemical Society
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200019 (Универзитет у Београду, Институт за примену нуклеарне енергије - ИНЕП) (RS-MESTD-inst-2020-200019)
Институција/група
IHTMTY - CONF AU - Šunderić, Miloš AU - Gligorijević, Nikola AU - Minić, Simeon AU - Nedić, Olgica AU - Nikolić, Milan PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7289 AB - In this study, the interaction between phycocyanobilin (PCB)1, a bioactive chromophore of blue-green algae Spirulina's phycobiliproteins, and alpha-2-macroglobulin (α2M)2, a universal anti-proteinase, was investigated under simulated physiological conditions using spectroscopic techniques and α2M activity assay. Using spectrofluorimetric measurements, we found that α2M binds PCB with a moderate affinity, with a binding constant of 6.3× 105 M−1 at 25°C. The binding of PCB to α2M does not cause any significant change in the secondary structure of the protein (circular dichroism measurements). Besides, PCB protects α2M from structural oxidative alterations under AAPH-induced free radical overproduction. Further, PCB binding effectively preserves α2M anti-proteinase activity. Since α2M is involved in controlling the action of enzymes during the inflammatory process, the protection that PCB expresses could indirectly influence the intensity and direction of body response to impaired homeostasis, especially under oxidative stress. PB - Serbian Biochemical Society C3 - Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia T1 - Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications SP - 163 EP - 163 UR - https://hdl.handle.net/21.15107/rcub_cer_7289 ER -
@conference{ author = "Šunderić, Miloš and Gligorijević, Nikola and Minić, Simeon and Nedić, Olgica and Nikolić, Milan", year = "2021", abstract = "In this study, the interaction between phycocyanobilin (PCB)1, a bioactive chromophore of blue-green algae Spirulina's phycobiliproteins, and alpha-2-macroglobulin (α2M)2, a universal anti-proteinase, was investigated under simulated physiological conditions using spectroscopic techniques and α2M activity assay. Using spectrofluorimetric measurements, we found that α2M binds PCB with a moderate affinity, with a binding constant of 6.3× 105 M−1 at 25°C. The binding of PCB to α2M does not cause any significant change in the secondary structure of the protein (circular dichroism measurements). Besides, PCB protects α2M from structural oxidative alterations under AAPH-induced free radical overproduction. Further, PCB binding effectively preserves α2M anti-proteinase activity. Since α2M is involved in controlling the action of enzymes during the inflammatory process, the protection that PCB expresses could indirectly influence the intensity and direction of body response to impaired homeostasis, especially under oxidative stress.", publisher = "Serbian Biochemical Society", journal = "Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia", title = "Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications", pages = "163-163", url = "https://hdl.handle.net/21.15107/rcub_cer_7289" }
Šunderić, M., Gligorijević, N., Minić, S., Nedić, O.,& Nikolić, M.. (2021). Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications. in Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia Serbian Biochemical Society., 163-163. https://hdl.handle.net/21.15107/rcub_cer_7289
Šunderić M, Gligorijević N, Minić S, Nedić O, Nikolić M. Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications. in Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia. 2021;:163-163. https://hdl.handle.net/21.15107/rcub_cer_7289 .
Šunderić, Miloš, Gligorijević, Nikola, Minić, Simeon, Nedić, Olgica, Nikolić, Milan, "Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications" in Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia (2021):163-163, https://hdl.handle.net/21.15107/rcub_cer_7289 .