Ovalbumin adsorption on different types of microplastic
Апстракт
Microplastics (MPs) are small in size, have low densities, can exist in the atmosphere for a
long time and can easily be spread by wind. Microplastics are plastic fibers, particles or films
with diameters smaller than 5 mm and they have shown different effects on proteins. The
objective of this study was to investigate adsorption affinity of different types of MPs
(polyethylene terephthalate (PET), polystyrene (PS) and polyvinyl chloride (PVC)) with
ovalbumin. Ovalbumin, isolated from chicken egg white, was used in this study. Plastics were
mixed with ovalbumin for 1,2,4 and 19 h and then the absorbance of the remaining protein in
the solution was measured at 280nm. In addition, isotherm mathematical model to calculate
the adsorption affinity of ovalbumin for MPs was used. We determined affinity constants by
using Langmuir isotherm models for different particle size (PS 120 m and PS 500 m),
different type of plastics (PET, PS and PVC) and pH values (3 and 7,2). Adsorption
ex...periment results showed that adsorption depends on type of plastics. Our results showed
that PVC did not adsorb protein, however, PET and PS have interacted with protein.
Adsorption capacities of all analysed MPs increase with pH of solution. Under different pH
values, MPs and protein have different charges that may affect adsorption characteristics.
With increase of pH from 3 to 7, the level of protein adsorption on MPs increased 14 times
for PS (smaller in size) and 5 times for PS (bigger size) and PET.
Кључне речи:
microplastics / ovalbuminИзвор:
FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia, 2021, 43-43Издавач:
- University of Belgrade - Faculty of Chemistry
Финансирање / пројекти:
- Serbian Academy of Sciences and Arts, grant number F-26
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics (EU-H2020-810752)
URI
https://ec.europa.eu/research/participants/documents/downloadPublic?documentIds=080166e5deeb546e&appId=PPGMShttps://cer.ihtm.bg.ac.rs/handle/123456789/7283
Институција/група
IHTMTY - CONF AU - Mutić, Tamara AU - Gligorijević, Nikola AU - Ćirković Veličković, Tanja PY - 2021 UR - https://ec.europa.eu/research/participants/documents/downloadPublic?documentIds=080166e5deeb546e&appId=PPGMS UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7283 AB - Microplastics (MPs) are small in size, have low densities, can exist in the atmosphere for a long time and can easily be spread by wind. Microplastics are plastic fibers, particles or films with diameters smaller than 5 mm and they have shown different effects on proteins. The objective of this study was to investigate adsorption affinity of different types of MPs (polyethylene terephthalate (PET), polystyrene (PS) and polyvinyl chloride (PVC)) with ovalbumin. Ovalbumin, isolated from chicken egg white, was used in this study. Plastics were mixed with ovalbumin for 1,2,4 and 19 h and then the absorbance of the remaining protein in the solution was measured at 280nm. In addition, isotherm mathematical model to calculate the adsorption affinity of ovalbumin for MPs was used. We determined affinity constants by using Langmuir isotherm models for different particle size (PS 120 m and PS 500 m), different type of plastics (PET, PS and PVC) and pH values (3 and 7,2). Adsorption experiment results showed that adsorption depends on type of plastics. Our results showed that PVC did not adsorb protein, however, PET and PS have interacted with protein. Adsorption capacities of all analysed MPs increase with pH of solution. Under different pH values, MPs and protein have different charges that may affect adsorption characteristics. With increase of pH from 3 to 7, the level of protein adsorption on MPs increased 14 times for PS (smaller in size) and 5 times for PS (bigger size) and PET. PB - University of Belgrade - Faculty of Chemistry C3 - FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia T1 - Ovalbumin adsorption on different types of microplastic SP - 43 EP - 43 UR - https://hdl.handle.net/21.15107/rcub_cer_7283 ER -
@conference{ author = "Mutić, Tamara and Gligorijević, Nikola and Ćirković Veličković, Tanja", year = "2021", abstract = "Microplastics (MPs) are small in size, have low densities, can exist in the atmosphere for a long time and can easily be spread by wind. Microplastics are plastic fibers, particles or films with diameters smaller than 5 mm and they have shown different effects on proteins. The objective of this study was to investigate adsorption affinity of different types of MPs (polyethylene terephthalate (PET), polystyrene (PS) and polyvinyl chloride (PVC)) with ovalbumin. Ovalbumin, isolated from chicken egg white, was used in this study. Plastics were mixed with ovalbumin for 1,2,4 and 19 h and then the absorbance of the remaining protein in the solution was measured at 280nm. In addition, isotherm mathematical model to calculate the adsorption affinity of ovalbumin for MPs was used. We determined affinity constants by using Langmuir isotherm models for different particle size (PS 120 m and PS 500 m), different type of plastics (PET, PS and PVC) and pH values (3 and 7,2). Adsorption experiment results showed that adsorption depends on type of plastics. Our results showed that PVC did not adsorb protein, however, PET and PS have interacted with protein. Adsorption capacities of all analysed MPs increase with pH of solution. Under different pH values, MPs and protein have different charges that may affect adsorption characteristics. With increase of pH from 3 to 7, the level of protein adsorption on MPs increased 14 times for PS (smaller in size) and 5 times for PS (bigger size) and PET.", publisher = "University of Belgrade - Faculty of Chemistry", journal = "FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia", title = "Ovalbumin adsorption on different types of microplastic", pages = "43-43", url = "https://hdl.handle.net/21.15107/rcub_cer_7283" }
Mutić, T., Gligorijević, N.,& Ćirković Veličković, T.. (2021). Ovalbumin adsorption on different types of microplastic. in FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia University of Belgrade - Faculty of Chemistry., 43-43. https://hdl.handle.net/21.15107/rcub_cer_7283
Mutić T, Gligorijević N, Ćirković Veličković T. Ovalbumin adsorption on different types of microplastic. in FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia. 2021;:43-43. https://hdl.handle.net/21.15107/rcub_cer_7283 .
Mutić, Tamara, Gligorijević, Nikola, Ćirković Veličković, Tanja, "Ovalbumin adsorption on different types of microplastic" in FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia (2021):43-43, https://hdl.handle.net/21.15107/rcub_cer_7283 .