Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications
Аутори
Solarz, DariaWitko, Tomasz
Karcz, Robert
Malagurski, Ivana
Ponjavić, Marijana
Levic, Steva
Nešić, Aleksandra
Guzik, Maciej
Savić, Sanja
Nikodinović-Runić, Jasmina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be p...otentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.
Кључне речи:
polylactic acid / polyhydroxyoctanoate / electrospinning / cell migration / biocompatibility / sustainability / sustainable developmentИзвор:
RSC Advances, 2023, 13, 34, 24112-24128Издавач:
- Royal Society of Chemistry (RSC)
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- European Union's Horizon 2020 research and innovation program under grant agreement No. 870292 (BioICEP)
- National Natural Science Foundation of China (No. 31961133016, 31961133015, and 31961133014)
- Polymeric material (PHO) was produced under grant TechMatStrateg no. TECHMATSTRATEG2/ 407507/1/NCBR/2019
DOI: 10.1039/D3RA03021K
ISSN: 2046-2069
PubMed: 37577093
WoS: 001045796400001
Scopus: 2-s2.0-85171023356
Институција/група
IHTMTY - JOUR AU - Solarz, Daria AU - Witko, Tomasz AU - Karcz, Robert AU - Malagurski, Ivana AU - Ponjavić, Marijana AU - Levic, Steva AU - Nešić, Aleksandra AU - Guzik, Maciej AU - Savić, Sanja AU - Nikodinović-Runić, Jasmina PY - 2023 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7170 AB - Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications. PB - Royal Society of Chemistry (RSC) T2 - RSC Advances T1 - Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications VL - 13 IS - 34 SP - 24112 EP - 24128 DO - 10.1039/D3RA03021K ER -
@article{ author = "Solarz, Daria and Witko, Tomasz and Karcz, Robert and Malagurski, Ivana and Ponjavić, Marijana and Levic, Steva and Nešić, Aleksandra and Guzik, Maciej and Savić, Sanja and Nikodinović-Runić, Jasmina", year = "2023", abstract = "Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.", publisher = "Royal Society of Chemistry (RSC)", journal = "RSC Advances", title = "Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications", volume = "13", number = "34", pages = "24112-24128", doi = "10.1039/D3RA03021K" }
Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances Royal Society of Chemistry (RSC)., 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K
Solarz D, Witko T, Karcz R, Malagurski I, Ponjavić M, Levic S, Nešić A, Guzik M, Savić S, Nikodinović-Runić J. Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances. 2023;13(34):24112-24128. doi:10.1039/D3RA03021K .
Solarz, Daria, Witko, Tomasz, Karcz, Robert, Malagurski, Ivana, Ponjavić, Marijana, Levic, Steva, Nešić, Aleksandra, Guzik, Maciej, Savić, Sanja, Nikodinović-Runić, Jasmina, "Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications" in RSC Advances, 13, no. 34 (2023):24112-24128, https://doi.org/10.1039/D3RA03021K . .