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Uporedna analiza izoformi α-amilaze iz srednjeg creva larvi Cerambyx cerdo L. (Coleoptera: Cerambycidae) iz prirode i gajenih na veštačkoj podlozi

dc.creatorDojnov, Biljana
dc.creatorLončar, Nikola
dc.creatorBožić, Nataša
dc.creatorNenadovic, Vera
dc.creatorIvanovic, Jelisaveta
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:24:03Z
dc.date.available2019-01-30T17:24:03Z
dc.date.issued2010
dc.identifier.issn0354-4664
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/708
dc.description.abstractα-Amylase isoforms of Cerambyx cerdo larvae from the wild (ML and SL) and reared in the laboratory (ADL) were compared. Three amylase isoforms were presented in the SL and ML extracts while two isoforms were presented in the ADL according to zymogram after isoelectric focusing (IEF). All C. cerdo amylase isoforms were acidic proteins (pI LT 3.5). Seven amylase isoforms (ACC 1-7) from the midgut of C. cerdo larvae were found in the ML midgut extract, six in the SL extract, and four in the ADL extract according to native PAGE zymogram. The ADL amylase had the highest activity. All crude midgut extracts of C. cerdo larvae were fractionated on a Superose 12 HR column. The molecular mass of the ACC was estimated to be 34 kDa.en
dc.description.abstractUpoređene su izoforme α-amilaze larvi Cerambyx cerdo sakupljenih iz prirode (ML i SL) i gajenih na veštačkoj podlozi u laboratoriji (ADL). Zimogramskom detekcijom posle IEF-a po tri izoforme su detektovane u ML i SL ekstraktima, a u ADL dve izoforme. Sve amilazne izoforme iz C. cerdo su bile kisele (pI LT 3.5). Zimogramskom detekcijom posle nativne elektroforeze sedam izoformi je detektovano u ML ekstraktu, šest u SL ekstraktu i četiri u ADL ekstraktu. Najveća amilazna aktivnost je detektovana u ADL ekstraktu. Svi ekstrakti srednjih creva larvi C. cerdo su frakcionisani na koloni Superose 12 HR. Molekulska masa ACC-a je bila 34 kDa.sr
dc.publisherInst Bioloska Istrazivanja Sinisa Stankovic, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.rightsopenAccess
dc.sourceArchives of Biological Sciences
dc.subjectCerambycidaeen
dc.subjectCerambyx cerdoen
dc.subjectmidguten
dc.subjectα-amylaseen
dc.subjectisoformen
dc.subjectzymogramen
dc.subjectxylophagous larvaeen
dc.titleComparison of α-amylase isoforms from the midgut of Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae developed in the wild and on an artificial dieten
dc.titleUporedna analiza izoformi α-amilaze iz srednjeg creva larvi Cerambyx cerdo L. (Coleoptera: Cerambycidae) iz prirode i gajenih na veštačkoj podlozisr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractЛонцар, Н.; Ненадовиц, Вера; Ивановиц, Јелисавета; Вујциц, З.; Дојнов, Биљана; Божић, Наташа;
dc.citation.volume62
dc.citation.issue3
dc.citation.spage575
dc.citation.epage584
dc.citation.other62(3): 575-584
dc.citation.rankM23
dc.identifier.doi10.2298/ABS1003575D
dc.identifier.rcubConv_2546
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/9324/706.pdf
dc.identifier.scopus2-s2.0-77955137452
dc.identifier.wos000281482000009
dc.type.versionpublishedVersion


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