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dc.creatorLončar, Nikola
dc.creatorVujčić, Zoran
dc.creatorBožić, Nataša
dc.creatorIvanovic, Jelisaveta
dc.creatorNenadovic, Vera
dc.date.accessioned2019-01-30T17:24:01Z
dc.date.available2019-01-30T17:24:01Z
dc.date.issued2010
dc.identifier.issn0739-4462
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/706
dc.description.abstractTrypsin-like enzyme (TLE) from the anterior midgut of Morimus funereus larvae was purified by anion exchange chromatography and gel filtration chromatography and characterized. Specific TLE activity was increased 322-fold by purification of the crude midgut extract. The purified enzyme had a pH optimum of 9.0 (optimum pH range 8.5-9.5) and temperature optimum of 45 degrees C with the KM ratio of 0.065 mM for benzoyl-arginine-p-nitroanilide (BApNA). Among a number of inhibitors tested, the most efficient was benzamidine (K-I value of 0.012 mM, Ic(50) value of 0.204 mM) while inhibition of TLE activity by SBTI, TLCK, and PMSF was partial. Almost all divalent cations tested enhanced the enzyme activity, amongst them Co2+ and Mn2+ stimulated TLE activity for 2.5 times. The purified TLE (after gel-filtration on Superose 12 column) had a molecular mass of 37.5 kDa with an isoelectric point over 9.3. Sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed one band of 38 kDa, suggesting that the enzyme is a monomer.en
dc.publisherWiley-Blackwell, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.rightsrestrictedAccess
dc.sourceArchives of Insect Biochemistry and Physiology
dc.subjecttrypsinen
dc.subjectmidguten
dc.subjectinhibitoren
dc.subjectCerambycid beetleen
dc.subjectMorimus funereusen
dc.subjectxylophagous larvaeen
dc.titlePurification and properties of trypsin-like enzyme from the midgut of morimus funereus (coleoptera, cerambycidae) larvaeen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractИвановиц, Јелисавета; Вујциц, Зоран; Лонцар, Никола; Божић, Наташа; Ненадовиц, Вера;
dc.citation.volume74
dc.citation.issue4
dc.citation.spage232
dc.citation.epage246
dc.citation.other74(4): 232-246
dc.citation.rankM21
dc.identifier.pmid20549815
dc.identifier.doi10.1002/arch.20371
dc.identifier.rcubConv_2544
dc.identifier.scopus2-s2.0-77957891727
dc.identifier.wos000281399500003
dc.type.versionpublishedVersion


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