Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells
Authorized Users Only
2010
Authors
Matić, Ivana Z.Žižak, Željko
Simonović, Mladen
Simonović, Branislav
Gođevac, Dejan
Savikin, Katarina
Juranić, Zorica
Article (Published version)
Metadata
Show full item recordAbstract
Red and white wine polyphenols have been reported to provide substantial health benefits. In this study, the cytotoxic activity of red and white wine polyphenolic extracts and of resveratrol was evaluated against different cancer cell lines-human cervix adenocarcinoma HeLa, human breast adenocarcinoma MDA-MB-361, and human breast carcinoma MDA-MB-453 and normal human peripheral blood mononuclear cells (PBMCs). Qualitative and quantitative compositions of wine polyphenolic extracts obtained by fractional vacuum distillation of corresponding wines were determined using spectrophotometric methods and high-performance liquid chromatography with diode array detection and liquid chromatography with electrospray ionization-time of flight mass spectrometry analysis. It was demonstrated that wine polyphenolic extracts and resveratrol exerted higher cytotoxic activity against HeLa and MDA-MB-453 cells in comparison to MDA-MB-361 cells and unstimulated and stimulated PBMCs. Furthermore, white win...e polyphenolic extract exhibited a significantly higher antiproliferative action on cancer cell lines than red wine extract. The presence of condensed or fragmented nuclei in HeLa cells, pretreated with extract of white wine and stained with a mixture of acridine orange and ethidium bromide, pointed to the morphological signs of apoptosis. In addition, HeLa cells in late stages of apoptosis or secondary necrosis were also observed. Results from our study suggest that polyphenolic extracts from red and white wine may have anticarcinogenic potential.
Keywords:
cancer cells / cytotoxicity / normal peripheral blood mononuclear cells / red wine polyphenols / white wine polyphenolsSource:
Journal of Medicinal Food, 2010, 13, 4, 851-862Publisher:
- Mary Ann Liebert Inc, New Rochelle
Funding / projects:
- Istraživanje dejstava modifikatora biološkog odgovora u fiziološkim i patološkim stanjima (RS-145006)
DOI: 10.1089/jmf.2009.0193
ISSN: 1096-620X
PubMed: 20482276
WoS: 000280485000014
Scopus: 2-s2.0-77955213675
Collections
Institution/Community
IHTMTY - JOUR AU - Matić, Ivana Z. AU - Žižak, Željko AU - Simonović, Mladen AU - Simonović, Branislav AU - Gođevac, Dejan AU - Savikin, Katarina AU - Juranić, Zorica PY - 2010 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/697 AB - Red and white wine polyphenols have been reported to provide substantial health benefits. In this study, the cytotoxic activity of red and white wine polyphenolic extracts and of resveratrol was evaluated against different cancer cell lines-human cervix adenocarcinoma HeLa, human breast adenocarcinoma MDA-MB-361, and human breast carcinoma MDA-MB-453 and normal human peripheral blood mononuclear cells (PBMCs). Qualitative and quantitative compositions of wine polyphenolic extracts obtained by fractional vacuum distillation of corresponding wines were determined using spectrophotometric methods and high-performance liquid chromatography with diode array detection and liquid chromatography with electrospray ionization-time of flight mass spectrometry analysis. It was demonstrated that wine polyphenolic extracts and resveratrol exerted higher cytotoxic activity against HeLa and MDA-MB-453 cells in comparison to MDA-MB-361 cells and unstimulated and stimulated PBMCs. Furthermore, white wine polyphenolic extract exhibited a significantly higher antiproliferative action on cancer cell lines than red wine extract. The presence of condensed or fragmented nuclei in HeLa cells, pretreated with extract of white wine and stained with a mixture of acridine orange and ethidium bromide, pointed to the morphological signs of apoptosis. In addition, HeLa cells in late stages of apoptosis or secondary necrosis were also observed. Results from our study suggest that polyphenolic extracts from red and white wine may have anticarcinogenic potential. PB - Mary Ann Liebert Inc, New Rochelle T2 - Journal of Medicinal Food T1 - Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells VL - 13 IS - 4 SP - 851 EP - 862 DO - 10.1089/jmf.2009.0193 ER -
@article{ author = "Matić, Ivana Z. and Žižak, Željko and Simonović, Mladen and Simonović, Branislav and Gođevac, Dejan and Savikin, Katarina and Juranić, Zorica", year = "2010", abstract = "Red and white wine polyphenols have been reported to provide substantial health benefits. In this study, the cytotoxic activity of red and white wine polyphenolic extracts and of resveratrol was evaluated against different cancer cell lines-human cervix adenocarcinoma HeLa, human breast adenocarcinoma MDA-MB-361, and human breast carcinoma MDA-MB-453 and normal human peripheral blood mononuclear cells (PBMCs). Qualitative and quantitative compositions of wine polyphenolic extracts obtained by fractional vacuum distillation of corresponding wines were determined using spectrophotometric methods and high-performance liquid chromatography with diode array detection and liquid chromatography with electrospray ionization-time of flight mass spectrometry analysis. It was demonstrated that wine polyphenolic extracts and resveratrol exerted higher cytotoxic activity against HeLa and MDA-MB-453 cells in comparison to MDA-MB-361 cells and unstimulated and stimulated PBMCs. Furthermore, white wine polyphenolic extract exhibited a significantly higher antiproliferative action on cancer cell lines than red wine extract. The presence of condensed or fragmented nuclei in HeLa cells, pretreated with extract of white wine and stained with a mixture of acridine orange and ethidium bromide, pointed to the morphological signs of apoptosis. In addition, HeLa cells in late stages of apoptosis or secondary necrosis were also observed. Results from our study suggest that polyphenolic extracts from red and white wine may have anticarcinogenic potential.", publisher = "Mary Ann Liebert Inc, New Rochelle", journal = "Journal of Medicinal Food", title = "Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells", volume = "13", number = "4", pages = "851-862", doi = "10.1089/jmf.2009.0193" }
Matić, I. Z., Žižak, Ž., Simonović, M., Simonović, B., Gođevac, D., Savikin, K.,& Juranić, Z.. (2010). Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells. in Journal of Medicinal Food Mary Ann Liebert Inc, New Rochelle., 13(4), 851-862. https://doi.org/10.1089/jmf.2009.0193
Matić IZ, Žižak Ž, Simonović M, Simonović B, Gođevac D, Savikin K, Juranić Z. Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells. in Journal of Medicinal Food. 2010;13(4):851-862. doi:10.1089/jmf.2009.0193 .
Matić, Ivana Z., Žižak, Željko, Simonović, Mladen, Simonović, Branislav, Gođevac, Dejan, Savikin, Katarina, Juranić, Zorica, "Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells" in Journal of Medicinal Food, 13, no. 4 (2010):851-862, https://doi.org/10.1089/jmf.2009.0193 . .