Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3
Само за регистроване кориснике
2024
Аутори
Khalife, MajdStanković, Dalibor
Stanković, Vesna
Danicka, Julia
Rizzotto, Francesco
Costache, Vlad
Schwok, Anny Slama
Gaudu, Philippe
Vidić, Jasmina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Vitamin K refers to a group of vitamins that play an important role in blood coagulation and regulation of bone and vascular metabolism. However, vitamin K3 may give severe side effects in animal and humans when improperly added to food and feed due to its toxicity. Here, an electrochemical biosensor, based on the YaiB NADPH-dependent quinone reductase from Lactococcus lactis (YaiB), was developed to achieve rapid and redox probe-free detection of vitamin K3. First, the ability of the carbon electrode to distinguish between 1,4-benzoquinone and hydroquinone was demonstrated. Then, we engineered YaiB to work as a bioreceptor immobilized at the electrode and its sensitivity and specificity to reduce vitamin K3 were demonstrated. Finally, to demonstrate the practical potential of the biosensor, we tested it directly in spiked milk samples, achieving 15-minute quantification of the vitamin K3. The limit of detection was 0.87 µM and 4.1 µM in buffer and milk, respectively.
Кључне речи:
Enzymatic sensor / Carbon Screen Printed Electrode / Voltammetry / Food quality / Vitamin detectionИзвор:
Food Chemistry, 2024, 433, 137316-Издавач:
- Elsevier
Институција/група
IHTMTY - JOUR AU - Khalife, Majd AU - Stanković, Dalibor AU - Stanković, Vesna AU - Danicka, Julia AU - Rizzotto, Francesco AU - Costache, Vlad AU - Schwok, Anny Slama AU - Gaudu, Philippe AU - Vidić, Jasmina PY - 2024 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6896 AB - Vitamin K refers to a group of vitamins that play an important role in blood coagulation and regulation of bone and vascular metabolism. However, vitamin K3 may give severe side effects in animal and humans when improperly added to food and feed due to its toxicity. Here, an electrochemical biosensor, based on the YaiB NADPH-dependent quinone reductase from Lactococcus lactis (YaiB), was developed to achieve rapid and redox probe-free detection of vitamin K3. First, the ability of the carbon electrode to distinguish between 1,4-benzoquinone and hydroquinone was demonstrated. Then, we engineered YaiB to work as a bioreceptor immobilized at the electrode and its sensitivity and specificity to reduce vitamin K3 were demonstrated. Finally, to demonstrate the practical potential of the biosensor, we tested it directly in spiked milk samples, achieving 15-minute quantification of the vitamin K3. The limit of detection was 0.87 µM and 4.1 µM in buffer and milk, respectively. PB - Elsevier T2 - Food Chemistry T1 - Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3 VL - 433 SP - 137316 DO - 10.1016/j.foodchem.2023.137316 ER -
@article{ author = "Khalife, Majd and Stanković, Dalibor and Stanković, Vesna and Danicka, Julia and Rizzotto, Francesco and Costache, Vlad and Schwok, Anny Slama and Gaudu, Philippe and Vidić, Jasmina", year = "2024", abstract = "Vitamin K refers to a group of vitamins that play an important role in blood coagulation and regulation of bone and vascular metabolism. However, vitamin K3 may give severe side effects in animal and humans when improperly added to food and feed due to its toxicity. Here, an electrochemical biosensor, based on the YaiB NADPH-dependent quinone reductase from Lactococcus lactis (YaiB), was developed to achieve rapid and redox probe-free detection of vitamin K3. First, the ability of the carbon electrode to distinguish between 1,4-benzoquinone and hydroquinone was demonstrated. Then, we engineered YaiB to work as a bioreceptor immobilized at the electrode and its sensitivity and specificity to reduce vitamin K3 were demonstrated. Finally, to demonstrate the practical potential of the biosensor, we tested it directly in spiked milk samples, achieving 15-minute quantification of the vitamin K3. The limit of detection was 0.87 µM and 4.1 µM in buffer and milk, respectively.", publisher = "Elsevier", journal = "Food Chemistry", title = "Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3", volume = "433", pages = "137316", doi = "10.1016/j.foodchem.2023.137316" }
Khalife, M., Stanković, D., Stanković, V., Danicka, J., Rizzotto, F., Costache, V., Schwok, A. S., Gaudu, P.,& Vidić, J.. (2024). Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3. in Food Chemistry Elsevier., 433, 137316. https://doi.org/10.1016/j.foodchem.2023.137316
Khalife M, Stanković D, Stanković V, Danicka J, Rizzotto F, Costache V, Schwok AS, Gaudu P, Vidić J. Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3. in Food Chemistry. 2024;433:137316. doi:10.1016/j.foodchem.2023.137316 .
Khalife, Majd, Stanković, Dalibor, Stanković, Vesna, Danicka, Julia, Rizzotto, Francesco, Costache, Vlad, Schwok, Anny Slama, Gaudu, Philippe, Vidić, Jasmina, "Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3" in Food Chemistry, 433 (2024):137316, https://doi.org/10.1016/j.foodchem.2023.137316 . .