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Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale

Promena izoprenoidne, steranske i terpanske frakcije tokom ex situ bioremedijacije mazuta na industrijskom nivou

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2010
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Authors
Beškoski, Vladimir
Takic, Milos
Milić, Jelena
Ilić, Mila
Gojgić-Cvijović, Gordana
Jovančićević, Branimir
Vrvić, Miroslav
Article (Published version)
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Abstract
This paper presents the results of an ex situ bioremediation of soil contaminated by mazut (heavy residual fuel oil) in the field scale (600 m3). The treatment-bed (thickness 0.4 m) consisted of mechanically mixed mazut-contaminated soil, softwood sawdust as an additional carbon source and crude river sand, as a bulking and porosity increasing material. The inoculation/rein-oculation was conducted periodically using a biomass of a consortium of zymogenous microorganisms isolated from a bioremediation substrate. The biostimulation was performed through addition of nutritious substances (N, P and K). The aeration was improved by systematic mixing of the bioremediation system. After 50 days, the number of hydrocarbon degraders had increased a 100 fold. Based on the changes in the group composition, the average biodegradation rate during bioremediation was 24 mg kg-1 day-1 for the aliphatic fraction, 6 mg kg-1 day-1 for the aromatic fraction and 3 mg kg-1 day-1 for the nitrogen-sulphur-oxy...gen compounds (NSO)-asphaltene fraction. In the saturated hydrocarbon fraction, gas chromatography-mass spectrometry (GC-MS) in the single ion-monitoring mode (SIM) was applied to analyze isoprenoids pristane and phytane and polycyclic molecules of sterane and triterpane type. Biodegradation occurred during the bioremediation process, as well as a reduction of the relative quantities of isoprenoids, steranes, tri- and tetracyclic terpanes and pentacyclic terpanes of the hopane type.

Prikazani su rezultati ex situ bioremedijacije zemljišta kontaminiranog mazutom na industrijskom nivou (600 m3). Bioremedijacioni materijal (debljine 0,4 m) se sastojao od mehanički pomešanih mazutom zagađenog zemljišta, čamove piljevine kao dodatnog izvora ugljenika i neprečišćenog rečnog peska, dodatog u cilju mešanja i povećanja poroznosti. Inokulacija/reinokulacija (bioaugmentacija) je periodično rađena sa biomasom konzorcijuma zimogenih mikrooorganizama izolovanih iz supstrata za bioremedijaciju. Biostimulacija je realizovana dodatkom hranljivih supstanci (N, P i K). Aeracija je poboljšavana sistematskim mešanjem bioremedijacionog sistema. Nakon 50 dana broj mikroorganizama koji degradiraju ugljovodonike povećan je 100 puta. Na osnovu promena u grupnom sastavu prosečna stopa biodegradacije tokom bioremedijacije je bila za alifatičnu frakciju 24 mg kg-1 po danu, za aromatičnu 6 mg kg-1 po danu i 3 mg kg-1 po danu za NSO-asfaltensku frakciju. U zasićenoj ugljovodoničnoj frakciji met...odom GC-MS (SIM metod) analizirani su izoprenoidi pristan i fitan i policiklični molekuli steranskog i triterpanskog tipa. Tokom bioremedijacionog procesa došlo je do biodegradacije i smanjenja relativnih količina izoprenoida, sterana, tri- i tetracikličnih terpana i pentacikličnih terpana hopanskog tipa.

Keywords:
mazut / bioremediation / field experiment / zymogenous microbial consortia / isoprenoids / steranes and terpanes
Source:
Journal of the Serbian Chemical Society, 2010, 75, 11, 1605-1616
Publisher:
  • Serbian Chemical Soc, Belgrade
Funding / projects:
  • Biomasa i metabolizam nekih mikroorganizama kao izvor široko upotrebljivih proizvoda i biohemijskih reakcija (RS-142018)
  • Proizvodni mobilni bioreaktor i dobijanje biomase mikroorganizama za bioremedijaciju (RS-20131)

DOI: 10.2298/JSC100505091B

ISSN: 0352-5139

WoS: 000285214100013

Scopus: 2-s2.0-78649614679
[ Google Scholar ]
7
8
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/686
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Beškoski, Vladimir
AU  - Takic, Milos
AU  - Milić, Jelena
AU  - Ilić, Mila
AU  - Gojgić-Cvijović, Gordana
AU  - Jovančićević, Branimir
AU  - Vrvić, Miroslav
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/686
AB  - This paper presents the results of an ex situ bioremediation of soil contaminated by mazut (heavy residual fuel oil) in the field scale (600 m3). The treatment-bed (thickness 0.4 m) consisted of mechanically mixed mazut-contaminated soil, softwood sawdust as an additional carbon source and crude river sand, as a bulking and porosity increasing material. The inoculation/rein-oculation was conducted periodically using a biomass of a consortium of zymogenous microorganisms isolated from a bioremediation substrate. The biostimulation was performed through addition of nutritious substances (N, P and K). The aeration was improved by systematic mixing of the bioremediation system. After 50 days, the number of hydrocarbon degraders had increased a 100 fold. Based on the changes in the group composition, the average biodegradation rate during bioremediation was 24 mg kg-1 day-1 for the aliphatic fraction, 6 mg kg-1 day-1 for the aromatic fraction and 3 mg kg-1 day-1 for the nitrogen-sulphur-oxygen compounds (NSO)-asphaltene fraction. In the saturated hydrocarbon fraction, gas chromatography-mass spectrometry (GC-MS) in the single ion-monitoring mode (SIM) was applied to analyze isoprenoids pristane and phytane and polycyclic molecules of sterane and triterpane type. Biodegradation occurred during the bioremediation process, as well as a reduction of the relative quantities of isoprenoids, steranes, tri- and tetracyclic terpanes and pentacyclic terpanes of the hopane type.
AB  - Prikazani su rezultati ex situ bioremedijacije zemljišta kontaminiranog mazutom na industrijskom nivou (600 m3). Bioremedijacioni materijal (debljine 0,4 m) se sastojao od mehanički pomešanih mazutom zagađenog zemljišta, čamove piljevine kao dodatnog izvora ugljenika i neprečišćenog rečnog peska, dodatog u cilju mešanja i povećanja poroznosti. Inokulacija/reinokulacija (bioaugmentacija) je periodično rađena sa biomasom konzorcijuma zimogenih mikrooorganizama izolovanih iz supstrata za bioremedijaciju. Biostimulacija je realizovana dodatkom hranljivih supstanci (N, P i K). Aeracija je poboljšavana sistematskim mešanjem bioremedijacionog sistema. Nakon 50 dana broj mikroorganizama koji degradiraju ugljovodonike povećan je 100 puta. Na osnovu promena u grupnom sastavu prosečna stopa biodegradacije tokom bioremedijacije je bila za alifatičnu frakciju 24 mg kg-1 po danu, za aromatičnu 6 mg kg-1 po danu i 3 mg kg-1 po danu za NSO-asfaltensku frakciju. U zasićenoj ugljovodoničnoj frakciji metodom GC-MS (SIM metod) analizirani su izoprenoidi pristan i fitan i policiklični molekuli steranskog i triterpanskog tipa. Tokom bioremedijacionog procesa došlo je do biodegradacije i smanjenja relativnih količina izoprenoida, sterana, tri- i tetracikličnih terpana i pentacikličnih terpana hopanskog tipa.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale
T1  - Promena izoprenoidne, steranske i terpanske frakcije tokom ex situ bioremedijacije mazuta na industrijskom nivou
VL  - 75
IS  - 11
SP  - 1605
EP  - 1616
DO  - 10.2298/JSC100505091B
ER  - 
@article{
author = "Beškoski, Vladimir and Takic, Milos and Milić, Jelena and Ilić, Mila and Gojgić-Cvijović, Gordana and Jovančićević, Branimir and Vrvić, Miroslav",
year = "2010",
abstract = "This paper presents the results of an ex situ bioremediation of soil contaminated by mazut (heavy residual fuel oil) in the field scale (600 m3). The treatment-bed (thickness 0.4 m) consisted of mechanically mixed mazut-contaminated soil, softwood sawdust as an additional carbon source and crude river sand, as a bulking and porosity increasing material. The inoculation/rein-oculation was conducted periodically using a biomass of a consortium of zymogenous microorganisms isolated from a bioremediation substrate. The biostimulation was performed through addition of nutritious substances (N, P and K). The aeration was improved by systematic mixing of the bioremediation system. After 50 days, the number of hydrocarbon degraders had increased a 100 fold. Based on the changes in the group composition, the average biodegradation rate during bioremediation was 24 mg kg-1 day-1 for the aliphatic fraction, 6 mg kg-1 day-1 for the aromatic fraction and 3 mg kg-1 day-1 for the nitrogen-sulphur-oxygen compounds (NSO)-asphaltene fraction. In the saturated hydrocarbon fraction, gas chromatography-mass spectrometry (GC-MS) in the single ion-monitoring mode (SIM) was applied to analyze isoprenoids pristane and phytane and polycyclic molecules of sterane and triterpane type. Biodegradation occurred during the bioremediation process, as well as a reduction of the relative quantities of isoprenoids, steranes, tri- and tetracyclic terpanes and pentacyclic terpanes of the hopane type., Prikazani su rezultati ex situ bioremedijacije zemljišta kontaminiranog mazutom na industrijskom nivou (600 m3). Bioremedijacioni materijal (debljine 0,4 m) se sastojao od mehanički pomešanih mazutom zagađenog zemljišta, čamove piljevine kao dodatnog izvora ugljenika i neprečišćenog rečnog peska, dodatog u cilju mešanja i povećanja poroznosti. Inokulacija/reinokulacija (bioaugmentacija) je periodično rađena sa biomasom konzorcijuma zimogenih mikrooorganizama izolovanih iz supstrata za bioremedijaciju. Biostimulacija je realizovana dodatkom hranljivih supstanci (N, P i K). Aeracija je poboljšavana sistematskim mešanjem bioremedijacionog sistema. Nakon 50 dana broj mikroorganizama koji degradiraju ugljovodonike povećan je 100 puta. Na osnovu promena u grupnom sastavu prosečna stopa biodegradacije tokom bioremedijacije je bila za alifatičnu frakciju 24 mg kg-1 po danu, za aromatičnu 6 mg kg-1 po danu i 3 mg kg-1 po danu za NSO-asfaltensku frakciju. U zasićenoj ugljovodoničnoj frakciji metodom GC-MS (SIM metod) analizirani su izoprenoidi pristan i fitan i policiklični molekuli steranskog i triterpanskog tipa. Tokom bioremedijacionog procesa došlo je do biodegradacije i smanjenja relativnih količina izoprenoida, sterana, tri- i tetracikličnih terpana i pentacikličnih terpana hopanskog tipa.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale, Promena izoprenoidne, steranske i terpanske frakcije tokom ex situ bioremedijacije mazuta na industrijskom nivou",
volume = "75",
number = "11",
pages = "1605-1616",
doi = "10.2298/JSC100505091B"
}
Beškoski, V., Takic, M., Milić, J., Ilić, M., Gojgić-Cvijović, G., Jovančićević, B.,& Vrvić, M.. (2010). Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 75(11), 1605-1616.
https://doi.org/10.2298/JSC100505091B
Beškoski V, Takic M, Milić J, Ilić M, Gojgić-Cvijović G, Jovančićević B, Vrvić M. Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale. in Journal of the Serbian Chemical Society. 2010;75(11):1605-1616.
doi:10.2298/JSC100505091B .
Beškoski, Vladimir, Takic, Milos, Milić, Jelena, Ilić, Mila, Gojgić-Cvijović, Gordana, Jovančićević, Branimir, Vrvić, Miroslav, "Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale" in Journal of the Serbian Chemical Society, 75, no. 11 (2010):1605-1616,
https://doi.org/10.2298/JSC100505091B . .

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