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Immobilization of Cell Wall Invertase Modified with Glutaraldehyde for Continuous Production of Invert Sugar
dc.creator | Vujčić, Zoran | |
dc.creator | Milovanović, Aleksandra | |
dc.creator | Božić, Nataša | |
dc.creator | Dojnov, Biljana | |
dc.creator | Vujčić, Miroslava | |
dc.creator | Anđelković, Uroš | |
dc.creator | Lončar, Nikola | |
dc.date.accessioned | 2019-01-30T17:23:25Z | |
dc.date.available | 2019-01-30T17:23:25Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0021-8561 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/678 | |
dc.description.abstract | Yeast cell wall invertase (CWI) was modified with dimethyl suberimidate, glutaraldehyde, formaldehyde, and sodium periodate. Retained activity after modification was 45% for CWI modified with formaldehyde, 77% for CWI modified with sodium periodate, 80% for CWI modified with glutaraldehyde, and 115% for CWI modified with dimethyl suberimidate. Chemically modified and native CWIs showed significantly broad pH stability (pH 3-11), whereas after incubations at 50, 60, and 70 degrees C, CWI modified with glutaraldehyde showed the highest thermostability. Optimum pH for CWI modified with glutaraldehyde was between 4 and 5, whereas optimum temperature was at 60 degrees C. Comparison to CWI modified with glutaraldehyde after immobilization within alginate beads showed broader pH optimum (4.0-5.5) as well as broader temperature optimum (55-70 degrees C). Column bed reactor packed with the immobilized CWI modified with glutaraldehyde was successfully used for the 95% inversion of 60% (w/w) sucrose at the flow rate of 3 bed volumes per hour, pH 4.9, and 45 degrees C. A 1 month productivity of 3844 kg of inverted sugar/kg of the immobilisate was obtained. | en |
dc.publisher | American Chemical Society (ACS) | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of Agricultural and Food Chemistry | |
dc.subject | Cell wall invertase | en |
dc.subject | immobilization | en |
dc.subject | alginate | en |
dc.subject | chemical modification | en |
dc.subject | glutaraidehyde | en |
dc.subject | invert sugar | en |
dc.title | Immobilization of Cell Wall Invertase Modified with Glutaraldehyde for Continuous Production of Invert Sugar | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Вујциц, Зоран; Дојнов, Биљана; Вујчић, Мирослава; Божић, Наташа; Милутиновић Николић, Aлександра; Лонцар, Никола; Aнђелковић, Урош; | |
dc.citation.volume | 58 | |
dc.citation.issue | 22 | |
dc.citation.spage | 11896 | |
dc.citation.epage | 11900 | |
dc.citation.other | 58(22): 11896-11900 | |
dc.citation.rank | aM21 | |
dc.identifier.pmid | 20977188 | |
dc.identifier.doi | 10.1021/jf101836r | |
dc.identifier.scopus | 2-s2.0-78751563079 | |
dc.identifier.wos | 000284203000054 | |
dc.type.version | publishedVersion |