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dc.creatorVujčić, Zoran
dc.creatorMilovanović, Aleksandra
dc.creatorBožić, Nataša
dc.creatorDojnov, Biljana
dc.creatorVujčić, Miroslava
dc.creatorAnđelković, Uroš
dc.creatorLončar, Nikola
dc.date.accessioned2019-01-30T17:23:25Z
dc.date.available2019-01-30T17:23:25Z
dc.date.issued2010
dc.identifier.issn0021-8561
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/678
dc.description.abstractYeast cell wall invertase (CWI) was modified with dimethyl suberimidate, glutaraldehyde, formaldehyde, and sodium periodate. Retained activity after modification was 45% for CWI modified with formaldehyde, 77% for CWI modified with sodium periodate, 80% for CWI modified with glutaraldehyde, and 115% for CWI modified with dimethyl suberimidate. Chemically modified and native CWIs showed significantly broad pH stability (pH 3-11), whereas after incubations at 50, 60, and 70 degrees C, CWI modified with glutaraldehyde showed the highest thermostability. Optimum pH for CWI modified with glutaraldehyde was between 4 and 5, whereas optimum temperature was at 60 degrees C. Comparison to CWI modified with glutaraldehyde after immobilization within alginate beads showed broader pH optimum (4.0-5.5) as well as broader temperature optimum (55-70 degrees C). Column bed reactor packed with the immobilized CWI modified with glutaraldehyde was successfully used for the 95% inversion of 60% (w/w) sucrose at the flow rate of 3 bed volumes per hour, pH 4.9, and 45 degrees C. A 1 month productivity of 3844 kg of inverted sugar/kg of the immobilisate was obtained.en
dc.publisherAmer Chemical Soc, Washington
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Agricultural and Food Chemistry
dc.subjectCell wall invertaseen
dc.subjectimmobilizationen
dc.subjectalginateen
dc.subjectchemical modificationen
dc.subjectglutaraidehydeen
dc.subjectinvert sugaren
dc.titleImmobilization of Cell Wall Invertase Modified with Glutaraldehyde for Continuous Production of Invert Sugaren
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВујциц, Зоран; Дојнов, Биљана; Вујчић, Мирослава; Божић, Наташа; Милутиновић Николић, Aлександра; Лонцар, Никола; Aнђелковић, Урош;
dc.citation.volume58
dc.citation.issue22
dc.citation.spage11896
dc.citation.epage11900
dc.citation.other58(22): 11896-11900
dc.citation.rankaM21
dc.identifier.pmid20977188
dc.identifier.doi10.1021/jf101836r
dc.identifier.rcubConv_2571
dc.identifier.scopus2-s2.0-78751563079
dc.identifier.wos000284203000054
dc.type.versionpublishedVersion


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