Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal
2023
Аутори
Radović, JelenaPopović, Dragana
Ćurčić, Tatjana
Nikolić, Milan
Minić, Simeon
Gligorijević, Nikola
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study aimed to improve chitosan polymer's capabilities to absorb mercury by
immobilising phycobiliproteins (PBPs) onto the surface of chitosan beads (chitosan–
PBPs). Phycobiliproteins, light-harvesting proteins from algae and cyanobacteria, have
several industrially essential applications. These proteins can bind heavy metals with high
affinities. Protein extracts obtained from both Arthrospira platensis, with C-phycocyanin
as the dominant phycobiliprotein and Neoporphyra haitanensis, with R-phycocyanin and
R-phycoerythrin as the dominant PBPs, were covalently immobilised onto chitosan beads.
Binding analysis showed that, on average, 54 μg of PBPs were immobilised per bead.
Immobilised proteins were still in their native state, with no visible colour change after
immobilisation. Chitosan–PBPs and chitosan alone were tested for mercury adsorption at
pH 4 and pH 7 by atomic absorption spectroscopy. The tested concentration range of
mercury was from 1 to 70 ppm. Affinity,... calculated using Henry's binding isotherm, of
chitosan–PBPs for mercury was higher at both pH values than chitosan alone.
Furthermore, chitosan–PBPs beads were able to absorb significantly more mercury than
chitosan alone. These results show that the covalent immobilisation of PBPs onto chitosan
improves its mercury adsorption characteristics and creates a more efficient eco-friendly
adsorbent for removing mercury ions in the tested concentration range.
Кључне речи:
phycobiliproteins / chitosan / mercury removalИзвор:
Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia, 2023, 116-116Издавач:
- Serbian Biochemical Society
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
Институција/група
IHTMTY - CONF AU - Radović, Jelena AU - Popović, Dragana AU - Ćurčić, Tatjana AU - Nikolić, Milan AU - Minić, Simeon AU - Gligorijević, Nikola PY - 2023 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6666 AB - This study aimed to improve chitosan polymer's capabilities to absorb mercury by immobilising phycobiliproteins (PBPs) onto the surface of chitosan beads (chitosan– PBPs). Phycobiliproteins, light-harvesting proteins from algae and cyanobacteria, have several industrially essential applications. These proteins can bind heavy metals with high affinities. Protein extracts obtained from both Arthrospira platensis, with C-phycocyanin as the dominant phycobiliprotein and Neoporphyra haitanensis, with R-phycocyanin and R-phycoerythrin as the dominant PBPs, were covalently immobilised onto chitosan beads. Binding analysis showed that, on average, 54 μg of PBPs were immobilised per bead. Immobilised proteins were still in their native state, with no visible colour change after immobilisation. Chitosan–PBPs and chitosan alone were tested for mercury adsorption at pH 4 and pH 7 by atomic absorption spectroscopy. The tested concentration range of mercury was from 1 to 70 ppm. Affinity, calculated using Henry's binding isotherm, of chitosan–PBPs for mercury was higher at both pH values than chitosan alone. Furthermore, chitosan–PBPs beads were able to absorb significantly more mercury than chitosan alone. These results show that the covalent immobilisation of PBPs onto chitosan improves its mercury adsorption characteristics and creates a more efficient eco-friendly adsorbent for removing mercury ions in the tested concentration range. PB - Serbian Biochemical Society C3 - Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia T1 - Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal SP - 116 EP - 116 UR - https://hdl.handle.net/21.15107/rcub_cer_6666 ER -
@conference{ author = "Radović, Jelena and Popović, Dragana and Ćurčić, Tatjana and Nikolić, Milan and Minić, Simeon and Gligorijević, Nikola", year = "2023", abstract = "This study aimed to improve chitosan polymer's capabilities to absorb mercury by immobilising phycobiliproteins (PBPs) onto the surface of chitosan beads (chitosan– PBPs). Phycobiliproteins, light-harvesting proteins from algae and cyanobacteria, have several industrially essential applications. These proteins can bind heavy metals with high affinities. Protein extracts obtained from both Arthrospira platensis, with C-phycocyanin as the dominant phycobiliprotein and Neoporphyra haitanensis, with R-phycocyanin and R-phycoerythrin as the dominant PBPs, were covalently immobilised onto chitosan beads. Binding analysis showed that, on average, 54 μg of PBPs were immobilised per bead. Immobilised proteins were still in their native state, with no visible colour change after immobilisation. Chitosan–PBPs and chitosan alone were tested for mercury adsorption at pH 4 and pH 7 by atomic absorption spectroscopy. The tested concentration range of mercury was from 1 to 70 ppm. Affinity, calculated using Henry's binding isotherm, of chitosan–PBPs for mercury was higher at both pH values than chitosan alone. Furthermore, chitosan–PBPs beads were able to absorb significantly more mercury than chitosan alone. These results show that the covalent immobilisation of PBPs onto chitosan improves its mercury adsorption characteristics and creates a more efficient eco-friendly adsorbent for removing mercury ions in the tested concentration range.", publisher = "Serbian Biochemical Society", journal = "Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia", title = "Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal", pages = "116-116", url = "https://hdl.handle.net/21.15107/rcub_cer_6666" }
Radović, J., Popović, D., Ćurčić, T., Nikolić, M., Minić, S.,& Gligorijević, N.. (2023). Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia Serbian Biochemical Society., 116-116. https://hdl.handle.net/21.15107/rcub_cer_6666
Radović J, Popović D, Ćurčić T, Nikolić M, Minić S, Gligorijević N. Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia. 2023;:116-116. https://hdl.handle.net/21.15107/rcub_cer_6666 .
Radović, Jelena, Popović, Dragana, Ćurčić, Tatjana, Nikolić, Milan, Minić, Simeon, Gligorijević, Nikola, "Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal" in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia (2023):116-116, https://hdl.handle.net/21.15107/rcub_cer_6666 .