Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress
2023
Аутори
Miljuš, GoranPenezić, Ana
Noe, Dragana
Dobrijević, Zorana
Baralić, Marko
Robajac, Dragana
Šunderić, Miloš
Gligorijević, Nikola
Dimitrijević, Ivan
Barišić, Goran
Nedić, Olgica
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Human serum tansferrin (hsTf) is a major circulatory protein crucial for the
transport/metabolism of Fe3+ ions. By sequestering and delivering ferric ions to target
tissues/cells hsTf maintains redox homeostasis. Oxidative stress (OS), one of the hallmarks
of (patho)physiological conditions, alters protein structure and function. The main role of
hsTf hinges on specific interaction with cellular Tf receptor (TfR) while other interactions
contribute to diverse functions. The aim of this study was to profile interacting partners of
hsTf in the samples of serum coming from patients diagnosed with a wide range of
pathological conditions with underlying OS status. Anti-hsTf pull-down samples were
analysed using mass spectrometry. Data went through analysis by appropriate
bioinformatic tools. Results reveal differences in expression of hsTf interacting proteins in
sample groups of patients suffering from kidney insufficiency subjected to dialysis
treatment (peritoneal-PD or hemo-H...D) also with patients with gestational diabetes
compared to respective healthy sample groups. Colorectal cancer stage T3 versus T2 stage
shows an inverse distribution of expression profiles in comparison to healthy samples.
Most prominent differences are seen in hsTf interacting partners involved in the
complement and coagulation cascades and cholesterol metabolic pathways, suggesting a
multifaceted role of hsTf in these processes throughout the course of the disease.
Кључне речи:
Human serum tansferrin / proteomics / Oxidative stressИзвор:
Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia, 2023, 69-69Издавач:
- Serbian Biochemical Society
Финансирање / пројекти:
- Science Fund of Republic of Serbia (Grant No. 6436701)
Институција/група
IHTMTY - CONF AU - Miljuš, Goran AU - Penezić, Ana AU - Noe, Dragana AU - Dobrijević, Zorana AU - Baralić, Marko AU - Robajac, Dragana AU - Šunderić, Miloš AU - Gligorijević, Nikola AU - Dimitrijević, Ivan AU - Barišić, Goran AU - Nedić, Olgica PY - 2023 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6664 AB - Human serum tansferrin (hsTf) is a major circulatory protein crucial for the transport/metabolism of Fe3+ ions. By sequestering and delivering ferric ions to target tissues/cells hsTf maintains redox homeostasis. Oxidative stress (OS), one of the hallmarks of (patho)physiological conditions, alters protein structure and function. The main role of hsTf hinges on specific interaction with cellular Tf receptor (TfR) while other interactions contribute to diverse functions. The aim of this study was to profile interacting partners of hsTf in the samples of serum coming from patients diagnosed with a wide range of pathological conditions with underlying OS status. Anti-hsTf pull-down samples were analysed using mass spectrometry. Data went through analysis by appropriate bioinformatic tools. Results reveal differences in expression of hsTf interacting proteins in sample groups of patients suffering from kidney insufficiency subjected to dialysis treatment (peritoneal-PD or hemo-HD) also with patients with gestational diabetes compared to respective healthy sample groups. Colorectal cancer stage T3 versus T2 stage shows an inverse distribution of expression profiles in comparison to healthy samples. Most prominent differences are seen in hsTf interacting partners involved in the complement and coagulation cascades and cholesterol metabolic pathways, suggesting a multifaceted role of hsTf in these processes throughout the course of the disease. PB - Serbian Biochemical Society C3 - Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia T1 - Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress SP - 69 EP - 69 UR - https://hdl.handle.net/21.15107/rcub_cer_6664 ER -
@conference{ author = "Miljuš, Goran and Penezić, Ana and Noe, Dragana and Dobrijević, Zorana and Baralić, Marko and Robajac, Dragana and Šunderić, Miloš and Gligorijević, Nikola and Dimitrijević, Ivan and Barišić, Goran and Nedić, Olgica", year = "2023", abstract = "Human serum tansferrin (hsTf) is a major circulatory protein crucial for the transport/metabolism of Fe3+ ions. By sequestering and delivering ferric ions to target tissues/cells hsTf maintains redox homeostasis. Oxidative stress (OS), one of the hallmarks of (patho)physiological conditions, alters protein structure and function. The main role of hsTf hinges on specific interaction with cellular Tf receptor (TfR) while other interactions contribute to diverse functions. The aim of this study was to profile interacting partners of hsTf in the samples of serum coming from patients diagnosed with a wide range of pathological conditions with underlying OS status. Anti-hsTf pull-down samples were analysed using mass spectrometry. Data went through analysis by appropriate bioinformatic tools. Results reveal differences in expression of hsTf interacting proteins in sample groups of patients suffering from kidney insufficiency subjected to dialysis treatment (peritoneal-PD or hemo-HD) also with patients with gestational diabetes compared to respective healthy sample groups. Colorectal cancer stage T3 versus T2 stage shows an inverse distribution of expression profiles in comparison to healthy samples. Most prominent differences are seen in hsTf interacting partners involved in the complement and coagulation cascades and cholesterol metabolic pathways, suggesting a multifaceted role of hsTf in these processes throughout the course of the disease.", publisher = "Serbian Biochemical Society", journal = "Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia", title = "Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress", pages = "69-69", url = "https://hdl.handle.net/21.15107/rcub_cer_6664" }
Miljuš, G., Penezić, A., Noe, D., Dobrijević, Z., Baralić, M., Robajac, D., Šunderić, M., Gligorijević, N., Dimitrijević, I., Barišić, G.,& Nedić, O.. (2023). Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia Serbian Biochemical Society., 69-69. https://hdl.handle.net/21.15107/rcub_cer_6664
Miljuš G, Penezić A, Noe D, Dobrijević Z, Baralić M, Robajac D, Šunderić M, Gligorijević N, Dimitrijević I, Barišić G, Nedić O. Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia. 2023;:69-69. https://hdl.handle.net/21.15107/rcub_cer_6664 .
Miljuš, Goran, Penezić, Ana, Noe, Dragana, Dobrijević, Zorana, Baralić, Marko, Robajac, Dragana, Šunderić, Miloš, Gligorijević, Nikola, Dimitrijević, Ivan, Barišić, Goran, Nedić, Olgica, "Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress" in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia (2023):69-69, https://hdl.handle.net/21.15107/rcub_cer_6664 .