Cytotoxic and Cell Cycle Effects Induced by Two Herbal Extracts on Human Cervix Carcinoma and Human Breast Cancer Cell Lines
Само за регистроване кориснике
2010
Аутори
Stanojković, TatjanaKonic-Ristic, Aleksandra
Juranić, Zorica
Savikin, Katarina
Zdunić, Gordana
Menković, Nebojša
Jadranin, Milka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In recent times interest has increased in the complementary medicine of cancer patients. Two herbal mixtures were prepared from 17 and 12 plants, respectively. The goal of this study was to examine the in vitro cytotoxic and cell cycle effects of the aqueous-ethanol extracts (Extract 1 and Extract 2) obtained by maceration of the mixtures. The two extracts investigated exhibited significant antiproliferative activity toward two human breast cancer cell lines (MDA-MB-361 and MDA-MB-453) and a human cervix carcinoma cell line (HeLa) with 50% inhibitory concentration (IC(50)) values ranging from 9.92 to 17.38 mu L/mL. The extracts did not exert any significant cytotoxicity toward healthy human peripheral blood mononuclear cells. In vitro antitumor activites were accompanied by an important apoptotic fraction of all cell lines after treatment with the extracts. The amount of total phenols was similar in both extracts, whereas the concentration of total tannins was significantly higher in E...xtract 1. Extract 1 was also found to be a stronger free radical scavenger, with an IC(50) value of 13.4 mu g/mL. Both extracts contained rosmarinic acid, while ursolic acid was identified in Extract 2.
Кључне речи:
apoptosis / 1,1-diphenyl-2-picrylhydrazyl / HeLa / MDA-MB-361 / MDA-MB-453 / peripheral blood mononuclear cells / phenolicsИзвор:
Journal of Medicinal Food, 2010, 13, 2, 291-297Издавач:
- Mary Ann Liebert Inc, New Rochelle
Финансирање / пројекти:
- Истраживање дејстава модификатора биолошког одговора у физиолошким и патолошким стањима (RS-MESTD-MPN2006-2010-145006)
DOI: 10.1089/jmf.2009.0086
ISSN: 1096-620X
PubMed: 20170382
WoS: 000277190700008
Scopus: 2-s2.0-77958183263
Институција/група
IHTMTY - JOUR AU - Stanojković, Tatjana AU - Konic-Ristic, Aleksandra AU - Juranić, Zorica AU - Savikin, Katarina AU - Zdunić, Gordana AU - Menković, Nebojša AU - Jadranin, Milka PY - 2010 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/659 AB - In recent times interest has increased in the complementary medicine of cancer patients. Two herbal mixtures were prepared from 17 and 12 plants, respectively. The goal of this study was to examine the in vitro cytotoxic and cell cycle effects of the aqueous-ethanol extracts (Extract 1 and Extract 2) obtained by maceration of the mixtures. The two extracts investigated exhibited significant antiproliferative activity toward two human breast cancer cell lines (MDA-MB-361 and MDA-MB-453) and a human cervix carcinoma cell line (HeLa) with 50% inhibitory concentration (IC(50)) values ranging from 9.92 to 17.38 mu L/mL. The extracts did not exert any significant cytotoxicity toward healthy human peripheral blood mononuclear cells. In vitro antitumor activites were accompanied by an important apoptotic fraction of all cell lines after treatment with the extracts. The amount of total phenols was similar in both extracts, whereas the concentration of total tannins was significantly higher in Extract 1. Extract 1 was also found to be a stronger free radical scavenger, with an IC(50) value of 13.4 mu g/mL. Both extracts contained rosmarinic acid, while ursolic acid was identified in Extract 2. PB - Mary Ann Liebert Inc, New Rochelle T2 - Journal of Medicinal Food T1 - Cytotoxic and Cell Cycle Effects Induced by Two Herbal Extracts on Human Cervix Carcinoma and Human Breast Cancer Cell Lines VL - 13 IS - 2 SP - 291 EP - 297 DO - 10.1089/jmf.2009.0086 ER -
@article{ author = "Stanojković, Tatjana and Konic-Ristic, Aleksandra and Juranić, Zorica and Savikin, Katarina and Zdunić, Gordana and Menković, Nebojša and Jadranin, Milka", year = "2010", abstract = "In recent times interest has increased in the complementary medicine of cancer patients. Two herbal mixtures were prepared from 17 and 12 plants, respectively. The goal of this study was to examine the in vitro cytotoxic and cell cycle effects of the aqueous-ethanol extracts (Extract 1 and Extract 2) obtained by maceration of the mixtures. The two extracts investigated exhibited significant antiproliferative activity toward two human breast cancer cell lines (MDA-MB-361 and MDA-MB-453) and a human cervix carcinoma cell line (HeLa) with 50% inhibitory concentration (IC(50)) values ranging from 9.92 to 17.38 mu L/mL. The extracts did not exert any significant cytotoxicity toward healthy human peripheral blood mononuclear cells. In vitro antitumor activites were accompanied by an important apoptotic fraction of all cell lines after treatment with the extracts. The amount of total phenols was similar in both extracts, whereas the concentration of total tannins was significantly higher in Extract 1. Extract 1 was also found to be a stronger free radical scavenger, with an IC(50) value of 13.4 mu g/mL. Both extracts contained rosmarinic acid, while ursolic acid was identified in Extract 2.", publisher = "Mary Ann Liebert Inc, New Rochelle", journal = "Journal of Medicinal Food", title = "Cytotoxic and Cell Cycle Effects Induced by Two Herbal Extracts on Human Cervix Carcinoma and Human Breast Cancer Cell Lines", volume = "13", number = "2", pages = "291-297", doi = "10.1089/jmf.2009.0086" }
Stanojković, T., Konic-Ristic, A., Juranić, Z., Savikin, K., Zdunić, G., Menković, N.,& Jadranin, M.. (2010). Cytotoxic and Cell Cycle Effects Induced by Two Herbal Extracts on Human Cervix Carcinoma and Human Breast Cancer Cell Lines. in Journal of Medicinal Food Mary Ann Liebert Inc, New Rochelle., 13(2), 291-297. https://doi.org/10.1089/jmf.2009.0086
Stanojković T, Konic-Ristic A, Juranić Z, Savikin K, Zdunić G, Menković N, Jadranin M. Cytotoxic and Cell Cycle Effects Induced by Two Herbal Extracts on Human Cervix Carcinoma and Human Breast Cancer Cell Lines. in Journal of Medicinal Food. 2010;13(2):291-297. doi:10.1089/jmf.2009.0086 .
Stanojković, Tatjana, Konic-Ristic, Aleksandra, Juranić, Zorica, Savikin, Katarina, Zdunić, Gordana, Menković, Nebojša, Jadranin, Milka, "Cytotoxic and Cell Cycle Effects Induced by Two Herbal Extracts on Human Cervix Carcinoma and Human Breast Cancer Cell Lines" in Journal of Medicinal Food, 13, no. 2 (2010):291-297, https://doi.org/10.1089/jmf.2009.0086 . .