Cytotoxicity of Multifunctional Composites of Amorphous Calcium Phosphate Containing Chitosan on Titanium Obtained by Novel in Situ Anodic Process
2021
Аутори
Pantović Pavlović, MarijanaPavlović, Miroslav
Nikolić, Nebojša D.
Kojić, Vesna V.
Stevanović, Jasmina
Panić, Vladimir
Ignjatović, Nenad
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Calcium phosphates (CP), amongst which amorphous calcium phosphate (ACP) and hydroxyapatite (HAp), along with Ti and TiO2 layers, have found vast applications in preventive and regenerative medicine due to their excellent biocompatibility, nontoxic properties and ability to participate in the normal metabolism of organisms. In this paper cytotoxicity of amorphous calcium phosphate (ACP) and chitosan lactate (ChOL) multifunctional and hybrid composite coatings on MRC-5 human lung fibroblast cell line is presented. ACP/TiO2 and ACP/TiO2/ChOL are deposited by coatings new in situ anodization/anaphoretic deposition process at constant voltage of 60 V for 180 s at 25 °C. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy MRC-5 cells in the Ti and ACP/TiO2 composite samples, while there was an increase in the number of viable cells in the ACP/TiO2/ChOL sample. There is improved cell proliferation, differentiation and cell viability in the later. Sampl...e containing ACP/TiO2/ChOL coating showed negative cytotoxicity in both DET and MTT tests. Greater recovery of MRC-5 human lung fibroblasts cells was observed compared to the control sample after 48 h of recovery. From these results, it can be concluded that not only the ACP/TiO2/ChOL multifunctional composite coating is non-cytotoxic, but the presence of ChOL in the coating improves cell proliferation, differentiation and cell viability. Based on the obtained results, it can be concluded that both composite materials used in the studies are non-cytotoxic to the cell lines used, and that 5 wt.% of ChOL has a positive effect on the non-toxicity of the material. Based on presented results in this paper and previous published results of the physicochemical and bioactive properties of the ACP/TiO2/ChOL composite material, it can be concluded that further development as well as potential preclinical studies would be largely justified.
Кључне речи:
cytotoxicity / dye exclusion test / colorimetric test with tetrazolium salts / amorphous calcium phosphate / chitosan oligolactateИзвор:
Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, March 17-19, 2021, Jahorina, Republic of Srpska, Bosnia and Herzegovina, 2021, 230-230Издавач:
- Zvornik : Faculty of Technology
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
Институција/група
IHTMTY - CONF AU - Pantović Pavlović, Marijana AU - Pavlović, Miroslav AU - Nikolić, Nebojša D. AU - Kojić, Vesna V. AU - Stevanović, Jasmina AU - Panić, Vladimir AU - Ignjatović, Nenad PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6561 AB - Calcium phosphates (CP), amongst which amorphous calcium phosphate (ACP) and hydroxyapatite (HAp), along with Ti and TiO2 layers, have found vast applications in preventive and regenerative medicine due to their excellent biocompatibility, nontoxic properties and ability to participate in the normal metabolism of organisms. In this paper cytotoxicity of amorphous calcium phosphate (ACP) and chitosan lactate (ChOL) multifunctional and hybrid composite coatings on MRC-5 human lung fibroblast cell line is presented. ACP/TiO2 and ACP/TiO2/ChOL are deposited by coatings new in situ anodization/anaphoretic deposition process at constant voltage of 60 V for 180 s at 25 °C. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy MRC-5 cells in the Ti and ACP/TiO2 composite samples, while there was an increase in the number of viable cells in the ACP/TiO2/ChOL sample. There is improved cell proliferation, differentiation and cell viability in the later. Sample containing ACP/TiO2/ChOL coating showed negative cytotoxicity in both DET and MTT tests. Greater recovery of MRC-5 human lung fibroblasts cells was observed compared to the control sample after 48 h of recovery. From these results, it can be concluded that not only the ACP/TiO2/ChOL multifunctional composite coating is non-cytotoxic, but the presence of ChOL in the coating improves cell proliferation, differentiation and cell viability. Based on the obtained results, it can be concluded that both composite materials used in the studies are non-cytotoxic to the cell lines used, and that 5 wt.% of ChOL has a positive effect on the non-toxicity of the material. Based on presented results in this paper and previous published results of the physicochemical and bioactive properties of the ACP/TiO2/ChOL composite material, it can be concluded that further development as well as potential preclinical studies would be largely justified. PB - Zvornik : Faculty of Technology C3 - Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, March 17-19, 2021, Jahorina, Republic of Srpska, Bosnia and Herzegovina T1 - Cytotoxicity of Multifunctional Composites of Amorphous Calcium Phosphate Containing Chitosan on Titanium Obtained by Novel in Situ Anodic Process SP - 230 EP - 230 UR - https://hdl.handle.net/21.15107/rcub_cer_6561 ER -
@conference{ author = "Pantović Pavlović, Marijana and Pavlović, Miroslav and Nikolić, Nebojša D. and Kojić, Vesna V. and Stevanović, Jasmina and Panić, Vladimir and Ignjatović, Nenad", year = "2021", abstract = "Calcium phosphates (CP), amongst which amorphous calcium phosphate (ACP) and hydroxyapatite (HAp), along with Ti and TiO2 layers, have found vast applications in preventive and regenerative medicine due to their excellent biocompatibility, nontoxic properties and ability to participate in the normal metabolism of organisms. In this paper cytotoxicity of amorphous calcium phosphate (ACP) and chitosan lactate (ChOL) multifunctional and hybrid composite coatings on MRC-5 human lung fibroblast cell line is presented. ACP/TiO2 and ACP/TiO2/ChOL are deposited by coatings new in situ anodization/anaphoretic deposition process at constant voltage of 60 V for 180 s at 25 °C. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy MRC-5 cells in the Ti and ACP/TiO2 composite samples, while there was an increase in the number of viable cells in the ACP/TiO2/ChOL sample. There is improved cell proliferation, differentiation and cell viability in the later. Sample containing ACP/TiO2/ChOL coating showed negative cytotoxicity in both DET and MTT tests. Greater recovery of MRC-5 human lung fibroblasts cells was observed compared to the control sample after 48 h of recovery. From these results, it can be concluded that not only the ACP/TiO2/ChOL multifunctional composite coating is non-cytotoxic, but the presence of ChOL in the coating improves cell proliferation, differentiation and cell viability. Based on the obtained results, it can be concluded that both composite materials used in the studies are non-cytotoxic to the cell lines used, and that 5 wt.% of ChOL has a positive effect on the non-toxicity of the material. Based on presented results in this paper and previous published results of the physicochemical and bioactive properties of the ACP/TiO2/ChOL composite material, it can be concluded that further development as well as potential preclinical studies would be largely justified.", publisher = "Zvornik : Faculty of Technology", journal = "Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, March 17-19, 2021, Jahorina, Republic of Srpska, Bosnia and Herzegovina", title = "Cytotoxicity of Multifunctional Composites of Amorphous Calcium Phosphate Containing Chitosan on Titanium Obtained by Novel in Situ Anodic Process", pages = "230-230", url = "https://hdl.handle.net/21.15107/rcub_cer_6561" }
Pantović Pavlović, M., Pavlović, M., Nikolić, N. D., Kojić, V. V., Stevanović, J., Panić, V.,& Ignjatović, N.. (2021). Cytotoxicity of Multifunctional Composites of Amorphous Calcium Phosphate Containing Chitosan on Titanium Obtained by Novel in Situ Anodic Process. in Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, March 17-19, 2021, Jahorina, Republic of Srpska, Bosnia and Herzegovina Zvornik : Faculty of Technology., 230-230. https://hdl.handle.net/21.15107/rcub_cer_6561
Pantović Pavlović M, Pavlović M, Nikolić ND, Kojić VV, Stevanović J, Panić V, Ignjatović N. Cytotoxicity of Multifunctional Composites of Amorphous Calcium Phosphate Containing Chitosan on Titanium Obtained by Novel in Situ Anodic Process. in Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, March 17-19, 2021, Jahorina, Republic of Srpska, Bosnia and Herzegovina. 2021;:230-230. https://hdl.handle.net/21.15107/rcub_cer_6561 .
Pantović Pavlović, Marijana, Pavlović, Miroslav, Nikolić, Nebojša D., Kojić, Vesna V., Stevanović, Jasmina, Panić, Vladimir, Ignjatović, Nenad, "Cytotoxicity of Multifunctional Composites of Amorphous Calcium Phosphate Containing Chitosan on Titanium Obtained by Novel in Situ Anodic Process" in Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, March 17-19, 2021, Jahorina, Republic of Srpska, Bosnia and Herzegovina (2021):230-230, https://hdl.handle.net/21.15107/rcub_cer_6561 .