Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients
Аутори
Marinković, ZoricaNikolić, Aleksandra
Stojanović, Srđan
Blagojević, Duško
Niketić, Vesna
Radunović, A,
Apostolski, S.
Spasić, Mihajlo
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to investigate the content of -SU groups and SOD activity m cerebrospinal fluid (CSF) both in ALS patients and control group, as well as to perform an in vitro lest for CSF NO binding capacity, in same examines. Nitric oxide for in vitro testing was provided by adding 40% sodium nitrite solution in the solution of 10% ferrous sulfate in 50% sulfuric acid. Our results showed significant increase -SH content in CSF of ALS patients in comparison with control group (114 ± 29 nmol -SH/mg proteins in CSF of ALS patients compared with 37 ± 12 nmol - SI Lmg proteins in CSF of controls)(p<0.05). After in vitro saturation of CSF with NO, we noted (he significant decrease of -SH content which was not found in control group. We also found that SOD activity in CSF of ALS patients was higher than in control group (16.1 ±2.8 U7 mg CSF proteins of ALS patients compared to 10.2 ± 2 U/ mg CSF proteins of control group). These results may confirm an increased ROS production in A...LS patients without increased NO production.
Кључне речи:
Superoxide dismutase activity / CSF / ALS / NO reactive speciesИзвор:
8th International Symposium on ALS/MND, Glasgow, 1997Институција/група
IHTMTY - CONF AU - Marinković, Zorica AU - Nikolić, Aleksandra AU - Stojanović, Srđan AU - Blagojević, Duško AU - Niketić, Vesna AU - Radunović, A, AU - Apostolski, S. AU - Spasić, Mihajlo PY - 1997 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6540 AB - The aim of this study was to investigate the content of -SU groups and SOD activity m cerebrospinal fluid (CSF) both in ALS patients and control group, as well as to perform an in vitro lest for CSF NO binding capacity, in same examines. Nitric oxide for in vitro testing was provided by adding 40% sodium nitrite solution in the solution of 10% ferrous sulfate in 50% sulfuric acid. Our results showed significant increase -SH content in CSF of ALS patients in comparison with control group (114 ± 29 nmol -SH/mg proteins in CSF of ALS patients compared with 37 ± 12 nmol - SI Lmg proteins in CSF of controls)(p<0.05). After in vitro saturation of CSF with NO, we noted (he significant decrease of -SH content which was not found in control group. We also found that SOD activity in CSF of ALS patients was higher than in control group (16.1 ±2.8 U7 mg CSF proteins of ALS patients compared to 10.2 ± 2 U/ mg CSF proteins of control group). These results may confirm an increased ROS production in ALS patients without increased NO production. C3 - 8th International Symposium on ALS/MND, Glasgow T1 - Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients UR - https://hdl.handle.net/21.15107/rcub_cer_6540 ER -
@conference{ author = "Marinković, Zorica and Nikolić, Aleksandra and Stojanović, Srđan and Blagojević, Duško and Niketić, Vesna and Radunović, A, and Apostolski, S. and Spasić, Mihajlo", year = "1997", abstract = "The aim of this study was to investigate the content of -SU groups and SOD activity m cerebrospinal fluid (CSF) both in ALS patients and control group, as well as to perform an in vitro lest for CSF NO binding capacity, in same examines. Nitric oxide for in vitro testing was provided by adding 40% sodium nitrite solution in the solution of 10% ferrous sulfate in 50% sulfuric acid. Our results showed significant increase -SH content in CSF of ALS patients in comparison with control group (114 ± 29 nmol -SH/mg proteins in CSF of ALS patients compared with 37 ± 12 nmol - SI Lmg proteins in CSF of controls)(p<0.05). After in vitro saturation of CSF with NO, we noted (he significant decrease of -SH content which was not found in control group. We also found that SOD activity in CSF of ALS patients was higher than in control group (16.1 ±2.8 U7 mg CSF proteins of ALS patients compared to 10.2 ± 2 U/ mg CSF proteins of control group). These results may confirm an increased ROS production in ALS patients without increased NO production.", journal = "8th International Symposium on ALS/MND, Glasgow", title = "Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients", url = "https://hdl.handle.net/21.15107/rcub_cer_6540" }
Marinković, Z., Nikolić, A., Stojanović, S., Blagojević, D., Niketić, V., Radunović, A., Apostolski, S.,& Spasić, M.. (1997). Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients. in 8th International Symposium on ALS/MND, Glasgow. https://hdl.handle.net/21.15107/rcub_cer_6540
Marinković Z, Nikolić A, Stojanović S, Blagojević D, Niketić V, Radunović A, Apostolski S, Spasić M. Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients. in 8th International Symposium on ALS/MND, Glasgow. 1997;. https://hdl.handle.net/21.15107/rcub_cer_6540 .
Marinković, Zorica, Nikolić, Aleksandra, Stojanović, Srđan, Blagojević, Duško, Niketić, Vesna, Radunović, A,, Apostolski, S., Spasić, Mihajlo, "Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients" in 8th International Symposium on ALS/MND, Glasgow (1997), https://hdl.handle.net/21.15107/rcub_cer_6540 .