Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this work a new spectrophotometric method for determination of nonenzymatically scrum protein-bound carbohydrates by phenol-sulfuric acid [5] as reagent, was used. All relevant parameters as the conditions of hydrolysis of ketoamine bond between glucose and protein, and formation 5-hydroxymethylfurfuraI (5-HMF) from glucose, as well as non specific influences on the reaction 5-HMF and •phenol-sulfuric acid, were investigated. Thus the determination of protein-bound carbohydrates by phenol-sulfuric acid was found more sensitive than spectrophotometric one which use thiobarbituric acid as reagent. More significant correlation with HbAlc content, determined by ion-exchange chromatography, was found. The non-specific influences on the determination was not found. Therefore, the determination of protein-bound carbohydrates by phenol sulfuric acid can serve as sensitive indicator of the degree hyperglycemia in diabetes.
Кључне речи:
serum / phenol-sulfuric acid / protein-bound carbohydratesИзвор:
Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece, 1998, PO407-PO407Издавач:
- Association of Greek Chemists
Институција/група
IHTMTY - CONF AU - Mandić, Ljuba AU - Stojanović, Srđan AU - Milin, Nenad AU - Čolović, Ana PY - 1998 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6539 AB - In this work a new spectrophotometric method for determination of nonenzymatically scrum protein-bound carbohydrates by phenol-sulfuric acid [5] as reagent, was used. All relevant parameters as the conditions of hydrolysis of ketoamine bond between glucose and protein, and formation 5-hydroxymethylfurfuraI (5-HMF) from glucose, as well as non specific influences on the reaction 5-HMF and •phenol-sulfuric acid, were investigated. Thus the determination of protein-bound carbohydrates by phenol-sulfuric acid was found more sensitive than spectrophotometric one which use thiobarbituric acid as reagent. More significant correlation with HbAlc content, determined by ion-exchange chromatography, was found. The non-specific influences on the determination was not found. Therefore, the determination of protein-bound carbohydrates by phenol sulfuric acid can serve as sensitive indicator of the degree hyperglycemia in diabetes. PB - Association of Greek Chemists C3 - Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece T1 - Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid SP - PO407 EP - PO407 UR - https://hdl.handle.net/21.15107/rcub_cer_6539 ER -
@conference{ author = "Mandić, Ljuba and Stojanović, Srđan and Milin, Nenad and Čolović, Ana", year = "1998", abstract = "In this work a new spectrophotometric method for determination of nonenzymatically scrum protein-bound carbohydrates by phenol-sulfuric acid [5] as reagent, was used. All relevant parameters as the conditions of hydrolysis of ketoamine bond between glucose and protein, and formation 5-hydroxymethylfurfuraI (5-HMF) from glucose, as well as non specific influences on the reaction 5-HMF and •phenol-sulfuric acid, were investigated. Thus the determination of protein-bound carbohydrates by phenol-sulfuric acid was found more sensitive than spectrophotometric one which use thiobarbituric acid as reagent. More significant correlation with HbAlc content, determined by ion-exchange chromatography, was found. The non-specific influences on the determination was not found. Therefore, the determination of protein-bound carbohydrates by phenol sulfuric acid can serve as sensitive indicator of the degree hyperglycemia in diabetes.", publisher = "Association of Greek Chemists", journal = "Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece", title = "Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid", pages = "PO407-PO407", url = "https://hdl.handle.net/21.15107/rcub_cer_6539" }
Mandić, L., Stojanović, S., Milin, N.,& Čolović, A.. (1998). Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid. in Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece Association of Greek Chemists., PO407-PO407. https://hdl.handle.net/21.15107/rcub_cer_6539
Mandić L, Stojanović S, Milin N, Čolović A. Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid. in Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece. 1998;:PO407-PO407. https://hdl.handle.net/21.15107/rcub_cer_6539 .
Mandić, Ljuba, Stojanović, Srđan, Milin, Nenad, Čolović, Ana, "Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid" in Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece (1998):PO407-PO407, https://hdl.handle.net/21.15107/rcub_cer_6539 .