Barley β-glucans: a new method for extraction and purification
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Barley β-glucans are non-starchy polysaccharides that are composed of linear chains of β-D-(1→3) and β-D-(1→4) linked glucose residues. They comprise three (cellotriosyl) or four (cellotetraosyl) consecutive β-D-(1→4) bonded glucose units mutually connected via β-D-(1→3) glycosidic bonds. These polysaccharides can be found in the aleurone layer of the barley grain endosperm 1. It has been shown that β-glucans reduce blood cholesterol and influence postprandial glucose levels which makes them health beneficial. Also, they have been used as stabilizing and thickening agents in the food indutry1,2. Due to the presence of β-D-(1→3) glycosidic bond, β-glucans are water soluble. The extraction procedure of these polysaccharides involves several steps that include the inactivation of endogenous enzymes found in the grain, extraction with water or alkali solutions, implementation of hydrolytic enzymes so that starch and protein removal is possible and precipitation of β-glucans from the purifi...ed solution with alcohol3. We have developed a new protocol that enables the extraction of β-glucans without the coextraction of starch and the implementation of hydrolytic enzymes for starch removal, which makes the process of purification shorter and less expensive. The extracted and purified glucans were further characterized by FTIR spectroscopy and microanalysis
Кључне речи:
β-glucans / barley / extraction / purificationИзвор:
Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022, 148-148Издавач:
- University of Belgrade - Faculty of Chemistry
- Serbian Biochemical Society
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
Институција/група
IHTMTY - CONF AU - Tomić, Nevena AU - Šokarda Slavić, Marinela AU - Ristović, Marina AU - Mišić, Milan AU - Vujčić, Zoran PY - 2022 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6457 AB - Barley β-glucans are non-starchy polysaccharides that are composed of linear chains of β-D-(1→3) and β-D-(1→4) linked glucose residues. They comprise three (cellotriosyl) or four (cellotetraosyl) consecutive β-D-(1→4) bonded glucose units mutually connected via β-D-(1→3) glycosidic bonds. These polysaccharides can be found in the aleurone layer of the barley grain endosperm 1. It has been shown that β-glucans reduce blood cholesterol and influence postprandial glucose levels which makes them health beneficial. Also, they have been used as stabilizing and thickening agents in the food indutry1,2. Due to the presence of β-D-(1→3) glycosidic bond, β-glucans are water soluble. The extraction procedure of these polysaccharides involves several steps that include the inactivation of endogenous enzymes found in the grain, extraction with water or alkali solutions, implementation of hydrolytic enzymes so that starch and protein removal is possible and precipitation of β-glucans from the purified solution with alcohol3. We have developed a new protocol that enables the extraction of β-glucans without the coextraction of starch and the implementation of hydrolytic enzymes for starch removal, which makes the process of purification shorter and less expensive. The extracted and purified glucans were further characterized by FTIR spectroscopy and microanalysis PB - University of Belgrade - Faculty of Chemistry PB - Serbian Biochemical Society C3 - Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia T1 - Barley β-glucans: a new method for extraction and purification SP - 148 EP - 148 UR - https://hdl.handle.net/21.15107/rcub_cer_6457 ER -
@conference{ author = "Tomić, Nevena and Šokarda Slavić, Marinela and Ristović, Marina and Mišić, Milan and Vujčić, Zoran", year = "2022", abstract = "Barley β-glucans are non-starchy polysaccharides that are composed of linear chains of β-D-(1→3) and β-D-(1→4) linked glucose residues. They comprise three (cellotriosyl) or four (cellotetraosyl) consecutive β-D-(1→4) bonded glucose units mutually connected via β-D-(1→3) glycosidic bonds. These polysaccharides can be found in the aleurone layer of the barley grain endosperm 1. It has been shown that β-glucans reduce blood cholesterol and influence postprandial glucose levels which makes them health beneficial. Also, they have been used as stabilizing and thickening agents in the food indutry1,2. Due to the presence of β-D-(1→3) glycosidic bond, β-glucans are water soluble. The extraction procedure of these polysaccharides involves several steps that include the inactivation of endogenous enzymes found in the grain, extraction with water or alkali solutions, implementation of hydrolytic enzymes so that starch and protein removal is possible and precipitation of β-glucans from the purified solution with alcohol3. We have developed a new protocol that enables the extraction of β-glucans without the coextraction of starch and the implementation of hydrolytic enzymes for starch removal, which makes the process of purification shorter and less expensive. The extracted and purified glucans were further characterized by FTIR spectroscopy and microanalysis", publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society", journal = "Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia", title = "Barley β-glucans: a new method for extraction and purification", pages = "148-148", url = "https://hdl.handle.net/21.15107/rcub_cer_6457" }
Tomić, N., Šokarda Slavić, M., Ristović, M., Mišić, M.,& Vujčić, Z.. (2022). Barley β-glucans: a new method for extraction and purification. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia University of Belgrade - Faculty of Chemistry., 148-148. https://hdl.handle.net/21.15107/rcub_cer_6457
Tomić N, Šokarda Slavić M, Ristović M, Mišić M, Vujčić Z. Barley β-glucans: a new method for extraction and purification. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia. 2022;:148-148. https://hdl.handle.net/21.15107/rcub_cer_6457 .
Tomić, Nevena, Šokarda Slavić, Marinela, Ristović, Marina, Mišić, Milan, Vujčić, Zoran, "Barley β-glucans: a new method for extraction and purification" in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia (2022):148-148, https://hdl.handle.net/21.15107/rcub_cer_6457 .