Electrochemical response of hemoglobin
Аутори
Mojović, ZoricaJović-Jovičić, Nataša
Ajduković, Marija
Marinović, Sanja
Mudrinić, Tihana
Ivanović-Šašić, Ana
Banković, Predrag
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Electron transfer between heme protein and electrode surface can be enhanced by electrode
modifiers such as clays. Using hemoglobin as sensor relays on its possibility to retain native
structure that enables hemoglobin to shift conformation. Therefore, electrochemical investigation
of hemoglobin response at extreme pH did not take attention. At extreme pH values protein
denaturation occurs resulting in conformation loss. The aim of this paper was to investigate
electrochemical behavior of hemoglobin supported on smectite in alkaline media. Hemoglobin
was immobilized on acid activated clay, bare and modified by SDS. The adsorption of hemoglobin
was slightly increased by the presence of SDS on the electrode surface. All samples showed
peak at -0.45 V ascribed to reduction pair of heme Fe(III)/Fe(II). Denaturation of hemoglobin
imparted chemically by SDS or electrochemically by cycling in cathodic potential range, led to
development of new peak at potential around -0.7 V in alka...line media. This feature might be
useful for studying and determination of different hemoglobin types.
Кључне речи:
Hemoglobin / Clay-modified electrodes / Electrochemical investigationИзвор:
Program and book of abstracts - Serbian Ceramic Society Conference – Advanced Ceramics and Application V, 21-23.09.2016, Belgrade, Serbia, 2016, 77-77Издавач:
- Serbian Ceramic Society
Финансирање / пројекти:
- Наноструктурни функционални и композитни материјали у каталитичким и сорпционим процесима (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45001)
Институција/група
IHTMTY - CONF AU - Mojović, Zorica AU - Jović-Jovičić, Nataša AU - Ajduković, Marija AU - Marinović, Sanja AU - Mudrinić, Tihana AU - Ivanović-Šašić, Ana AU - Banković, Predrag PY - 2016 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6335 AB - Electron transfer between heme protein and electrode surface can be enhanced by electrode modifiers such as clays. Using hemoglobin as sensor relays on its possibility to retain native structure that enables hemoglobin to shift conformation. Therefore, electrochemical investigation of hemoglobin response at extreme pH did not take attention. At extreme pH values protein denaturation occurs resulting in conformation loss. The aim of this paper was to investigate electrochemical behavior of hemoglobin supported on smectite in alkaline media. Hemoglobin was immobilized on acid activated clay, bare and modified by SDS. The adsorption of hemoglobin was slightly increased by the presence of SDS on the electrode surface. All samples showed peak at -0.45 V ascribed to reduction pair of heme Fe(III)/Fe(II). Denaturation of hemoglobin imparted chemically by SDS or electrochemically by cycling in cathodic potential range, led to development of new peak at potential around -0.7 V in alkaline media. This feature might be useful for studying and determination of different hemoglobin types. PB - Serbian Ceramic Society C3 - Program and book of abstracts - Serbian Ceramic Society Conference – Advanced Ceramics and Application V, 21-23.09.2016, Belgrade, Serbia T1 - Electrochemical response of hemoglobin SP - 77 EP - 77 UR - https://hdl.handle.net/21.15107/rcub_cer_6335 ER -
@conference{ author = "Mojović, Zorica and Jović-Jovičić, Nataša and Ajduković, Marija and Marinović, Sanja and Mudrinić, Tihana and Ivanović-Šašić, Ana and Banković, Predrag", year = "2016", abstract = "Electron transfer between heme protein and electrode surface can be enhanced by electrode modifiers such as clays. Using hemoglobin as sensor relays on its possibility to retain native structure that enables hemoglobin to shift conformation. Therefore, electrochemical investigation of hemoglobin response at extreme pH did not take attention. At extreme pH values protein denaturation occurs resulting in conformation loss. The aim of this paper was to investigate electrochemical behavior of hemoglobin supported on smectite in alkaline media. Hemoglobin was immobilized on acid activated clay, bare and modified by SDS. The adsorption of hemoglobin was slightly increased by the presence of SDS on the electrode surface. All samples showed peak at -0.45 V ascribed to reduction pair of heme Fe(III)/Fe(II). Denaturation of hemoglobin imparted chemically by SDS or electrochemically by cycling in cathodic potential range, led to development of new peak at potential around -0.7 V in alkaline media. This feature might be useful for studying and determination of different hemoglobin types.", publisher = "Serbian Ceramic Society", journal = "Program and book of abstracts - Serbian Ceramic Society Conference – Advanced Ceramics and Application V, 21-23.09.2016, Belgrade, Serbia", title = "Electrochemical response of hemoglobin", pages = "77-77", url = "https://hdl.handle.net/21.15107/rcub_cer_6335" }
Mojović, Z., Jović-Jovičić, N., Ajduković, M., Marinović, S., Mudrinić, T., Ivanović-Šašić, A.,& Banković, P.. (2016). Electrochemical response of hemoglobin. in Program and book of abstracts - Serbian Ceramic Society Conference – Advanced Ceramics and Application V, 21-23.09.2016, Belgrade, Serbia Serbian Ceramic Society., 77-77. https://hdl.handle.net/21.15107/rcub_cer_6335
Mojović Z, Jović-Jovičić N, Ajduković M, Marinović S, Mudrinić T, Ivanović-Šašić A, Banković P. Electrochemical response of hemoglobin. in Program and book of abstracts - Serbian Ceramic Society Conference – Advanced Ceramics and Application V, 21-23.09.2016, Belgrade, Serbia. 2016;:77-77. https://hdl.handle.net/21.15107/rcub_cer_6335 .
Mojović, Zorica, Jović-Jovičić, Nataša, Ajduković, Marija, Marinović, Sanja, Mudrinić, Tihana, Ivanović-Šašić, Ana, Banković, Predrag, "Electrochemical response of hemoglobin" in Program and book of abstracts - Serbian Ceramic Society Conference – Advanced Ceramics and Application V, 21-23.09.2016, Belgrade, Serbia (2016):77-77, https://hdl.handle.net/21.15107/rcub_cer_6335 .