EPR spin-trapping and spin-probing spectroscopy in assessing antioxidant properties: Example on extracts of catkin, leaves, and spiny burs of castanea sativa
Само за регистроване кориснике
2009
Аутори
Živković, JelenaZeković, Z.
Mujić, I.
Gođevac, Dejan
Mojović, Miloš
Mujić, A.
Spasojević, Ivan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Electron paramagnetic resonance (EPR) spin-trapping and spin-probing techniques were applied to determine antioxidant activity of extracts of catkin, leaves, and spiny burs of Castanea sativa against physiologically relevant reactive species-superoxide and hydroxyl radical generated in simple chemical systems and hydrogen peroxide applied on erythrocytes. Efflux of K + was used as a marker of membrane integrity. Chemical composition of extracts was analyzed using HPLC/DAD and LC/MS. Extracts showed high antioxidative capacity against superoxide but lower activity against hydroxyl radical. They protected fluidity and integrity of membranes of erythrocytes exposed to hydrogen peroxide. Levels of derivatives of ellagitannins showed positive correlation with the antioxidative activity of extracts. Therefore, ellagitannins from chestnut extracts could represent easily accessible natural antioxidants and beneficial component of human diet in pathophysiological conditions related to oxidative... stress. In conclusion, EPR spectroscopy represents a valuable tool for evaluation of antioxidant activity in both hydrophilic and lipophilic media.
Кључне речи:
Antioxidant / Castanea sativa / Ellagitannins / EPR / Oxidative stressИзвор:
Food Biophysics, 2009, 4, 2, 126-133Финансирање / пројекти:
DOI: 10.1007/s11483-009-9109-8
ISSN: 1557-1858
WoS: 000266007700008
Scopus: 2-s2.0-67349213556
Институција/група
IHTMTY - JOUR AU - Živković, Jelena AU - Zeković, Z. AU - Mujić, I. AU - Gođevac, Dejan AU - Mojović, Miloš AU - Mujić, A. AU - Spasojević, Ivan PY - 2009 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/609 AB - Electron paramagnetic resonance (EPR) spin-trapping and spin-probing techniques were applied to determine antioxidant activity of extracts of catkin, leaves, and spiny burs of Castanea sativa against physiologically relevant reactive species-superoxide and hydroxyl radical generated in simple chemical systems and hydrogen peroxide applied on erythrocytes. Efflux of K + was used as a marker of membrane integrity. Chemical composition of extracts was analyzed using HPLC/DAD and LC/MS. Extracts showed high antioxidative capacity against superoxide but lower activity against hydroxyl radical. They protected fluidity and integrity of membranes of erythrocytes exposed to hydrogen peroxide. Levels of derivatives of ellagitannins showed positive correlation with the antioxidative activity of extracts. Therefore, ellagitannins from chestnut extracts could represent easily accessible natural antioxidants and beneficial component of human diet in pathophysiological conditions related to oxidative stress. In conclusion, EPR spectroscopy represents a valuable tool for evaluation of antioxidant activity in both hydrophilic and lipophilic media. T2 - Food Biophysics T1 - EPR spin-trapping and spin-probing spectroscopy in assessing antioxidant properties: Example on extracts of catkin, leaves, and spiny burs of castanea sativa VL - 4 IS - 2 SP - 126 EP - 133 DO - 10.1007/s11483-009-9109-8 ER -
@article{ author = "Živković, Jelena and Zeković, Z. and Mujić, I. and Gođevac, Dejan and Mojović, Miloš and Mujić, A. and Spasojević, Ivan", year = "2009", abstract = "Electron paramagnetic resonance (EPR) spin-trapping and spin-probing techniques were applied to determine antioxidant activity of extracts of catkin, leaves, and spiny burs of Castanea sativa against physiologically relevant reactive species-superoxide and hydroxyl radical generated in simple chemical systems and hydrogen peroxide applied on erythrocytes. Efflux of K + was used as a marker of membrane integrity. Chemical composition of extracts was analyzed using HPLC/DAD and LC/MS. Extracts showed high antioxidative capacity against superoxide but lower activity against hydroxyl radical. They protected fluidity and integrity of membranes of erythrocytes exposed to hydrogen peroxide. Levels of derivatives of ellagitannins showed positive correlation with the antioxidative activity of extracts. Therefore, ellagitannins from chestnut extracts could represent easily accessible natural antioxidants and beneficial component of human diet in pathophysiological conditions related to oxidative stress. In conclusion, EPR spectroscopy represents a valuable tool for evaluation of antioxidant activity in both hydrophilic and lipophilic media.", journal = "Food Biophysics", title = "EPR spin-trapping and spin-probing spectroscopy in assessing antioxidant properties: Example on extracts of catkin, leaves, and spiny burs of castanea sativa", volume = "4", number = "2", pages = "126-133", doi = "10.1007/s11483-009-9109-8" }
Živković, J., Zeković, Z., Mujić, I., Gođevac, D., Mojović, M., Mujić, A.,& Spasojević, I.. (2009). EPR spin-trapping and spin-probing spectroscopy in assessing antioxidant properties: Example on extracts of catkin, leaves, and spiny burs of castanea sativa. in Food Biophysics, 4(2), 126-133. https://doi.org/10.1007/s11483-009-9109-8
Živković J, Zeković Z, Mujić I, Gođevac D, Mojović M, Mujić A, Spasojević I. EPR spin-trapping and spin-probing spectroscopy in assessing antioxidant properties: Example on extracts of catkin, leaves, and spiny burs of castanea sativa. in Food Biophysics. 2009;4(2):126-133. doi:10.1007/s11483-009-9109-8 .
Živković, Jelena, Zeković, Z., Mujić, I., Gođevac, Dejan, Mojović, Miloš, Mujić, A., Spasojević, Ivan, "EPR spin-trapping and spin-probing spectroscopy in assessing antioxidant properties: Example on extracts of catkin, leaves, and spiny burs of castanea sativa" in Food Biophysics, 4, no. 2 (2009):126-133, https://doi.org/10.1007/s11483-009-9109-8 . .