The use of enzymes may improve the functional properties of various food ingredients. The aim of this study was to examine the effects of proteolytic contaminants in phenol oxidases on β-lactoglobulin (BLG). In the presence of Trametes versicolor laccase and Agaricus bisporus tyrosinase, both variants of BLG (A and B) underwent removal of a peptide from the N-terminus. The truncated forms were more susceptible to digestion by pepsin. The truncation of BLG resulted from contaminating proteases and not due to the action of phenol oxidases. The removal of N-terminal peptides proceeded quickly, while the rest of the globular protein remained resistant to proteolysis for up to 3 h. In the case of the application of enzymes in food bioprocessing, it may be important to carefully monitor the effects of contaminating proteases in enzyme preparations used.
Source:
International Dairy Journal, 2009, 19, 12, 746-752
TY - JOUR
AU - Stanić, Dragana
AU - Radosavljevic, J.
AU - Polović, Natalija
AU - Jadranin, Milka
AU - Popović, M.
AU - Vuckovic, O.
AU - Burazer, L.
AU - Jankov, R.
AU - Ćirković Veličković, Tanja
PY - 2009
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/608
AB - The use of enzymes may improve the functional properties of various food ingredients. The aim of this study was to examine the effects of proteolytic contaminants in phenol oxidases on β-lactoglobulin (BLG). In the presence of Trametes versicolor laccase and Agaricus bisporus tyrosinase, both variants of BLG (A and B) underwent removal of a peptide from the N-terminus. The truncated forms were more susceptible to digestion by pepsin. The truncation of BLG resulted from contaminating proteases and not due to the action of phenol oxidases. The removal of N-terminal peptides proceeded quickly, while the rest of the globular protein remained resistant to proteolysis for up to 3 h. In the case of the application of enzymes in food bioprocessing, it may be important to carefully monitor the effects of contaminating proteases in enzyme preparations used.
T2 - International Dairy Journal
T1 - Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation
VL - 19
IS - 12
SP - 746
EP - 752
DO - 10.1016/j.idairyj.2009.05.008
ER -
@article{
author = "Stanić, Dragana and Radosavljevic, J. and Polović, Natalija and Jadranin, Milka and Popović, M. and Vuckovic, O. and Burazer, L. and Jankov, R. and Ćirković Veličković, Tanja",
year = "2009",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/608",
abstract = "The use of enzymes may improve the functional properties of various food ingredients. The aim of this study was to examine the effects of proteolytic contaminants in phenol oxidases on β-lactoglobulin (BLG). In the presence of Trametes versicolor laccase and Agaricus bisporus tyrosinase, both variants of BLG (A and B) underwent removal of a peptide from the N-terminus. The truncated forms were more susceptible to digestion by pepsin. The truncation of BLG resulted from contaminating proteases and not due to the action of phenol oxidases. The removal of N-terminal peptides proceeded quickly, while the rest of the globular protein remained resistant to proteolysis for up to 3 h. In the case of the application of enzymes in food bioprocessing, it may be important to carefully monitor the effects of contaminating proteases in enzyme preparations used.",
journal = "International Dairy Journal",
title = "Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation",
volume = "19",
number = "12",
pages = "746-752",
doi = "10.1016/j.idairyj.2009.05.008"
}
Stanić, D., Radosavljevic, J., Polović, N., Jadranin, M., Popović, M., Vuckovic, O., Burazer, L., Jankov, R.,& Ćirković Veličković, T. (2009). Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation.
International Dairy Journal, 19(12), 746-752.
https://doi.org/10.1016/j.idairyj.2009.05.008
Stanić D, Radosavljevic J, Polović N, Jadranin M, Popović M, Vuckovic O, Burazer L, Jankov R, Ćirković Veličković T. Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation. International Dairy Journal. 2009;19(12):746-752
Stanić Dragana, Radosavljevic J., Polović Natalija, Jadranin Milka, Popović M., Vuckovic O., Burazer L., Jankov R., Ćirković Veličković Tanja, "Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation" International Dairy Journal, 19, no. 12 (2009):746-752,
https://doi.org/10.1016/j.idairyj.2009.05.008 .
