DNA Photolyase: Molecular Machinery for Repair of UV-Induced Damage in DNA
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Damages in DNA structure are often caused by UV light, ionizing radiation, toxic substances and
environmental pollution. To maintain genetic stability, cells protect themselves against these kinds
of lesions. Moreover, the main DNA repair processes in prokaryotic and eukaryotic cells are quite
similar. Photolyases repair the major DNA defects-cyclobutane pyrimidine dimers (CPD) and
(6-4)-photoproducts. The enzyme contains two photoactive cofactors: folate-the photon antenna
molecule and catalytically active FADH-form. Photolyase repairs UV (200-300 nm) induced damage in DNA by splitting the ring of CPD dimer into pyrimidine monomers. If not repaired the CPD lesions are highly cytotoxic, mutagenic, and carcinogenic. In the present theoretical/computational study of photolyase from E. coli, continuum electrostatic and electron tunneling currents methods are employed to get a full insight into photoactivation and
photorepair mechanism of the enzyme and the structure-function i...nterrelation. Protonation state
of titratable residues, redox potentials of the conserved tryptophan triad, the energetics and kinetic
reaction rates are calculated comparing well with available experimental data. The free energies of
all potentially relevant enzyme states during the photoreactivation mechanism are evaluated. This presentation also addresses a several long-time controversial questions about the biological
relevance of unusual U-shape of FADH cofactor; hopping vs. super-exchange mechanism of the ET pathway; the shortest FADH- to CPD distance, after flipping out the CPD damage to the active site, in the CPD-protein complex or presence of radical FADH* state in the resting state of photolyase. The study could be relevant for other types of photolyases and cryptochromes, which all share the same structural features.
Ključne reči:
DNA / DNA damage / computational studyIzvor:
6th Postdoctoral Research Symposium, March 30, 2021, Davis, CA, USA, Web symposium, 2021, 33-Izdavač:
- USA, CA : UC Davis
Institucija/grupa
IHTMTY - CONF AU - Popović, Dragan AU - Stuchebrukhov, Alexei PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5877 AB - Damages in DNA structure are often caused by UV light, ionizing radiation, toxic substances and environmental pollution. To maintain genetic stability, cells protect themselves against these kinds of lesions. Moreover, the main DNA repair processes in prokaryotic and eukaryotic cells are quite similar. Photolyases repair the major DNA defects-cyclobutane pyrimidine dimers (CPD) and (6-4)-photoproducts. The enzyme contains two photoactive cofactors: folate-the photon antenna molecule and catalytically active FADH-form. Photolyase repairs UV (200-300 nm) induced damage in DNA by splitting the ring of CPD dimer into pyrimidine monomers. If not repaired the CPD lesions are highly cytotoxic, mutagenic, and carcinogenic. In the present theoretical/computational study of photolyase from E. coli, continuum electrostatic and electron tunneling currents methods are employed to get a full insight into photoactivation and photorepair mechanism of the enzyme and the structure-function interrelation. Protonation state of titratable residues, redox potentials of the conserved tryptophan triad, the energetics and kinetic reaction rates are calculated comparing well with available experimental data. The free energies of all potentially relevant enzyme states during the photoreactivation mechanism are evaluated. This presentation also addresses a several long-time controversial questions about the biological relevance of unusual U-shape of FADH cofactor; hopping vs. super-exchange mechanism of the ET pathway; the shortest FADH- to CPD distance, after flipping out the CPD damage to the active site, in the CPD-protein complex or presence of radical FADH* state in the resting state of photolyase. The study could be relevant for other types of photolyases and cryptochromes, which all share the same structural features. PB - USA, CA : UC Davis C3 - 6th Postdoctoral Research Symposium, March 30, 2021, Davis, CA, USA, Web symposium T1 - DNA Photolyase: Molecular Machinery for Repair of UV-Induced Damage in DNA SP - 33 UR - https://hdl.handle.net/21.15107/rcub_cer_5877 ER -
@conference{ author = "Popović, Dragan and Stuchebrukhov, Alexei", year = "2021", abstract = "Damages in DNA structure are often caused by UV light, ionizing radiation, toxic substances and environmental pollution. To maintain genetic stability, cells protect themselves against these kinds of lesions. Moreover, the main DNA repair processes in prokaryotic and eukaryotic cells are quite similar. Photolyases repair the major DNA defects-cyclobutane pyrimidine dimers (CPD) and (6-4)-photoproducts. The enzyme contains two photoactive cofactors: folate-the photon antenna molecule and catalytically active FADH-form. Photolyase repairs UV (200-300 nm) induced damage in DNA by splitting the ring of CPD dimer into pyrimidine monomers. If not repaired the CPD lesions are highly cytotoxic, mutagenic, and carcinogenic. In the present theoretical/computational study of photolyase from E. coli, continuum electrostatic and electron tunneling currents methods are employed to get a full insight into photoactivation and photorepair mechanism of the enzyme and the structure-function interrelation. Protonation state of titratable residues, redox potentials of the conserved tryptophan triad, the energetics and kinetic reaction rates are calculated comparing well with available experimental data. The free energies of all potentially relevant enzyme states during the photoreactivation mechanism are evaluated. This presentation also addresses a several long-time controversial questions about the biological relevance of unusual U-shape of FADH cofactor; hopping vs. super-exchange mechanism of the ET pathway; the shortest FADH- to CPD distance, after flipping out the CPD damage to the active site, in the CPD-protein complex or presence of radical FADH* state in the resting state of photolyase. The study could be relevant for other types of photolyases and cryptochromes, which all share the same structural features.", publisher = "USA, CA : UC Davis", journal = "6th Postdoctoral Research Symposium, March 30, 2021, Davis, CA, USA, Web symposium", title = "DNA Photolyase: Molecular Machinery for Repair of UV-Induced Damage in DNA", pages = "33", url = "https://hdl.handle.net/21.15107/rcub_cer_5877" }
Popović, D.,& Stuchebrukhov, A.. (2021). DNA Photolyase: Molecular Machinery for Repair of UV-Induced Damage in DNA. in 6th Postdoctoral Research Symposium, March 30, 2021, Davis, CA, USA, Web symposium USA, CA : UC Davis., 33. https://hdl.handle.net/21.15107/rcub_cer_5877
Popović D, Stuchebrukhov A. DNA Photolyase: Molecular Machinery for Repair of UV-Induced Damage in DNA. in 6th Postdoctoral Research Symposium, March 30, 2021, Davis, CA, USA, Web symposium. 2021;:33. https://hdl.handle.net/21.15107/rcub_cer_5877 .
Popović, Dragan, Stuchebrukhov, Alexei, "DNA Photolyase: Molecular Machinery for Repair of UV-Induced Damage in DNA" in 6th Postdoctoral Research Symposium, March 30, 2021, Davis, CA, USA, Web symposium (2021):33, https://hdl.handle.net/21.15107/rcub_cer_5877 .