Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate
Само за регистроване кориснике
2021
Аутори
Stojanović, SanjaStepanović, Jelena
Margetić, Aleksandra
Duduk, Nataša
Dojnov, Biljana
Vujčić, Zoran
Duduk, Bojan
Конференцијски прилог (Објављена верзија)
,
Sociedade Portuguesa de Química
Метаподаци
Приказ свих података о документуАпстракт
Fructooligosaccharides (FOS) are used in the human diet thanks to their prebiotic effect and they are generally regarded as safe [1]. The increase in the functional food that contains prebiotics, has been tremendous over the last two decades. The most suitable process for FOS synthesis is by enzymes [2], particularly the one produced by filamentous fungi from the genus Aspergillus [2,3]. Aspergillus welwitschiae FAW1 strain, isolated from the environment, has proven to be non-toxigenic and safe for use in food production. Moreover, the absence of ochratoxins and fumonisins production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters. Growing fungi on the natural substrate, triticale (Triticosecale sp), led to the production of various enzymes from inulinase complex (InuA, InuE, FTase, FFase). Production of FOS has been tested in two possible ways with the obtained inulinase enzyme complex: (1) synthesis from sucrose – fructo...syltransferase activity and (2) hydrolysis of inulin – endoinulinase and exoinulinase activity. The obtained FOS were detected by TLC and HPLC methods and characterized by examination of antioxidant capacity. Produced FOS showed significant antioxidant potential according to ABTS and ORAC which classifies them as important additives in functional food. These findings open up opportunities for an easy approach for FOS production by fungal inulinase enzymes, without their prior separation and purification.
Кључне речи:
FOS / inulinase / AspergillusИзвор:
Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line, 2021, 172-Издавач:
- Sociedade Portuguesa de Química
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200214 (Институт за пестициде и заштиту животне средине, Београд) (RS-MESTD-inst-2020-200214)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
Институција/група
IHTMTY - CONF AU - Stojanović, Sanja AU - Stepanović, Jelena AU - Margetić, Aleksandra AU - Duduk, Nataša AU - Dojnov, Biljana AU - Vujčić, Zoran AU - Duduk, Bojan PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5816 AB - Fructooligosaccharides (FOS) are used in the human diet thanks to their prebiotic effect and they are generally regarded as safe [1]. The increase in the functional food that contains prebiotics, has been tremendous over the last two decades. The most suitable process for FOS synthesis is by enzymes [2], particularly the one produced by filamentous fungi from the genus Aspergillus [2,3]. Aspergillus welwitschiae FAW1 strain, isolated from the environment, has proven to be non-toxigenic and safe for use in food production. Moreover, the absence of ochratoxins and fumonisins production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters. Growing fungi on the natural substrate, triticale (Triticosecale sp), led to the production of various enzymes from inulinase complex (InuA, InuE, FTase, FFase). Production of FOS has been tested in two possible ways with the obtained inulinase enzyme complex: (1) synthesis from sucrose – fructosyltransferase activity and (2) hydrolysis of inulin – endoinulinase and exoinulinase activity. The obtained FOS were detected by TLC and HPLC methods and characterized by examination of antioxidant capacity. Produced FOS showed significant antioxidant potential according to ABTS and ORAC which classifies them as important additives in functional food. These findings open up opportunities for an easy approach for FOS production by fungal inulinase enzymes, without their prior separation and purification. PB - Sociedade Portuguesa de Química C3 - Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line T1 - Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate SP - 172 UR - https://hdl.handle.net/21.15107/rcub_cer_5816 ER -
@conference{ author = "Stojanović, Sanja and Stepanović, Jelena and Margetić, Aleksandra and Duduk, Nataša and Dojnov, Biljana and Vujčić, Zoran and Duduk, Bojan", year = "2021", abstract = "Fructooligosaccharides (FOS) are used in the human diet thanks to their prebiotic effect and they are generally regarded as safe [1]. The increase in the functional food that contains prebiotics, has been tremendous over the last two decades. The most suitable process for FOS synthesis is by enzymes [2], particularly the one produced by filamentous fungi from the genus Aspergillus [2,3]. Aspergillus welwitschiae FAW1 strain, isolated from the environment, has proven to be non-toxigenic and safe for use in food production. Moreover, the absence of ochratoxins and fumonisins production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters. Growing fungi on the natural substrate, triticale (Triticosecale sp), led to the production of various enzymes from inulinase complex (InuA, InuE, FTase, FFase). Production of FOS has been tested in two possible ways with the obtained inulinase enzyme complex: (1) synthesis from sucrose – fructosyltransferase activity and (2) hydrolysis of inulin – endoinulinase and exoinulinase activity. The obtained FOS were detected by TLC and HPLC methods and characterized by examination of antioxidant capacity. Produced FOS showed significant antioxidant potential according to ABTS and ORAC which classifies them as important additives in functional food. These findings open up opportunities for an easy approach for FOS production by fungal inulinase enzymes, without their prior separation and purification.", publisher = "Sociedade Portuguesa de Química", journal = "Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line", title = "Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate", pages = "172", url = "https://hdl.handle.net/21.15107/rcub_cer_5816" }
Stojanović, S., Stepanović, J., Margetić, A., Duduk, N., Dojnov, B., Vujčić, Z.,& Duduk, B.. (2021). Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate. in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line Sociedade Portuguesa de Química., 172. https://hdl.handle.net/21.15107/rcub_cer_5816
Stojanović S, Stepanović J, Margetić A, Duduk N, Dojnov B, Vujčić Z, Duduk B. Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate. in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line. 2021;:172. https://hdl.handle.net/21.15107/rcub_cer_5816 .
Stojanović, Sanja, Stepanović, Jelena, Margetić, Aleksandra, Duduk, Nataša, Dojnov, Biljana, Vujčić, Zoran, Duduk, Bojan, "Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate" in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line (2021):172, https://hdl.handle.net/21.15107/rcub_cer_5816 .