Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms
Authors
Đukanović, Stefana
Ganić, Tea
Lončarević, Branka

Cvetković, Stefana

Nikolić, Biljana

Tenji, Dina
Randjelović, Danijela

Mitić-Ćulafić, Dragana

Article (Published version)
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Aims Because the Staphylococcus aureus is one of the most well‐known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it. Methods and Results Antibacterial activity was examined through microdilution assay. Antibiofilm testing included crystal violet staining of biofilm biomass and morphology analysis by Atomic force microscopy (AFM). Furthermore, aerobic respiration was monitored using the Micro‐Oxymax respirometer. For investigation of gene expression qRT‐PCR was performed. Emodin demonstrated strong antibacterial activity and ability to inhibit biofilm formation of all tested strains. The effect on preformed biofilms was spotted in few strains. AFM revealed that emodin affects biofilm structure and roughness. Monitoring of respiration under emodin treatment in planktonic and biofilm form revealed that emodin influenced aerobic respiration. Moreover, qRT‐PCR showed that emodin modulates e...xpression of icaA, icaD, srrA and srrB genes, as well as RNAIII, and that this activity was strain‐specific. Conclusion The results obtained in this study indicate the novel antibiofilm activity of emodin and its multiple pathways of action. Significance and Impact of Study This is the first study that examined pathways through which emodin expressed its antibiofilm activity
Keywords:
aerobic respiration / AFM / biofilm / emodin / Staphylococcus aureusSource:
Journal of Applied Microbiology, 2022, 132, 3, 1840-1855Publisher:
- Wiley
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200178 (University of Belgrade, Faculty of Biology) (RS-200178)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-200026)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200125 (University of Novi Sad, Faculty of Science) (RS-200125)
DOI: 10.1111/jam.15360
ISSN: 1364-5072
PubMed: 34779074
WoS: 000721912100001
Scopus: 2-s2.0-85119837365
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IHTMTY - JOUR AU - Đukanović, Stefana AU - Ganić, Tea AU - Lončarević, Branka AU - Cvetković, Stefana AU - Nikolić, Biljana AU - Tenji, Dina AU - Randjelović, Danijela AU - Mitić-Ćulafić, Dragana PY - 2022 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5590 AB - Aims Because the Staphylococcus aureus is one of the most well‐known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it. Methods and Results Antibacterial activity was examined through microdilution assay. Antibiofilm testing included crystal violet staining of biofilm biomass and morphology analysis by Atomic force microscopy (AFM). Furthermore, aerobic respiration was monitored using the Micro‐Oxymax respirometer. For investigation of gene expression qRT‐PCR was performed. Emodin demonstrated strong antibacterial activity and ability to inhibit biofilm formation of all tested strains. The effect on preformed biofilms was spotted in few strains. AFM revealed that emodin affects biofilm structure and roughness. Monitoring of respiration under emodin treatment in planktonic and biofilm form revealed that emodin influenced aerobic respiration. Moreover, qRT‐PCR showed that emodin modulates expression of icaA, icaD, srrA and srrB genes, as well as RNAIII, and that this activity was strain‐specific. Conclusion The results obtained in this study indicate the novel antibiofilm activity of emodin and its multiple pathways of action. Significance and Impact of Study This is the first study that examined pathways through which emodin expressed its antibiofilm activity PB - Wiley T2 - Journal of Applied Microbiology T1 - Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms VL - 132 IS - 3 SP - 1840 EP - 1855 DO - 10.1111/jam.15360 ER -
@article{ author = "Đukanović, Stefana and Ganić, Tea and Lončarević, Branka and Cvetković, Stefana and Nikolić, Biljana and Tenji, Dina and Randjelović, Danijela and Mitić-Ćulafić, Dragana", year = "2022", abstract = "Aims Because the Staphylococcus aureus is one of the most well‐known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it. Methods and Results Antibacterial activity was examined through microdilution assay. Antibiofilm testing included crystal violet staining of biofilm biomass and morphology analysis by Atomic force microscopy (AFM). Furthermore, aerobic respiration was monitored using the Micro‐Oxymax respirometer. For investigation of gene expression qRT‐PCR was performed. Emodin demonstrated strong antibacterial activity and ability to inhibit biofilm formation of all tested strains. The effect on preformed biofilms was spotted in few strains. AFM revealed that emodin affects biofilm structure and roughness. Monitoring of respiration under emodin treatment in planktonic and biofilm form revealed that emodin influenced aerobic respiration. Moreover, qRT‐PCR showed that emodin modulates expression of icaA, icaD, srrA and srrB genes, as well as RNAIII, and that this activity was strain‐specific. Conclusion The results obtained in this study indicate the novel antibiofilm activity of emodin and its multiple pathways of action. Significance and Impact of Study This is the first study that examined pathways through which emodin expressed its antibiofilm activity", publisher = "Wiley", journal = "Journal of Applied Microbiology", title = "Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms", volume = "132", number = "3", pages = "1840-1855", doi = "10.1111/jam.15360" }
Đukanović, S., Ganić, T., Lončarević, B., Cvetković, S., Nikolić, B., Tenji, D., Randjelović, D.,& Mitić-Ćulafić, D.. (2022). Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms. in Journal of Applied Microbiology Wiley., 132(3), 1840-1855. https://doi.org/10.1111/jam.15360
Đukanović S, Ganić T, Lončarević B, Cvetković S, Nikolić B, Tenji D, Randjelović D, Mitić-Ćulafić D. Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms. in Journal of Applied Microbiology. 2022;132(3):1840-1855. doi:10.1111/jam.15360 .
Đukanović, Stefana, Ganić, Tea, Lončarević, Branka, Cvetković, Stefana, Nikolić, Biljana, Tenji, Dina, Randjelović, Danijela, Mitić-Ćulafić, Dragana, "Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms" in Journal of Applied Microbiology, 132, no. 3 (2022):1840-1855, https://doi.org/10.1111/jam.15360 . .