Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization,but none pay attention to the isoenzyme composition of commercial HRP or the influence of thecarbohydrate component of the protein molecule on the biomineralization process. To study theimpact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-Cisoform for biomineralization within ZIF-8. With purified HRP, enzyme@ZIF-8 biocomposites withhigher activity were obtained, while periodate oxidation of the carbohydrate component of bothcommercial HRP and purified HRP-C yields biocomposites with very high activity in acetate bufferthat does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent thefalse high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At thesame time, purification and especially oxidation of the carbohydrate component of enzymes prior tobiomineralization lead to significantly improved a...ctivity of the biocomposites.
TY - JOUR
AU - Stanišić, Marija D.
AU - Popović Kokar, Nikolina
AU - Ristić, Predrag
AU - Balaž, Ana Marija
AU - Ognjanović, Miloš
AU - Đokić, Veljko R.
AU - Prodanović, Radivoje
AU - Todorović, Tamara
PY - 2022
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5561
AB - Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization,but none pay attention to the isoenzyme composition of commercial HRP or the influence of thecarbohydrate component of the protein molecule on the biomineralization process. To study theimpact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-Cisoform for biomineralization within ZIF-8. With purified HRP, enzyme@ZIF-8 biocomposites withhigher activity were obtained, while periodate oxidation of the carbohydrate component of bothcommercial HRP and purified HRP-C yields biocomposites with very high activity in acetate bufferthat does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent thefalse high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At thesame time, purification and especially oxidation of the carbohydrate component of enzymes prior tobiomineralization lead to significantly improved activity of the biocomposites.
PB - MDPI
T2 - Polymers
T1 - The influence of isoenzymes composition and chemical modification on horseradish peroxidase@ZIF-8 bio-composite performance
VL - 14
SP - 4834
DO - 10.3390/polym14224834
ER -
@article{
author = "Stanišić, Marija D. and Popović Kokar, Nikolina and Ristić, Predrag and Balaž, Ana Marija and Ognjanović, Miloš and Đokić, Veljko R. and Prodanović, Radivoje and Todorović, Tamara",
year = "2022",
abstract = "Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization,but none pay attention to the isoenzyme composition of commercial HRP or the influence of thecarbohydrate component of the protein molecule on the biomineralization process. To study theimpact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-Cisoform for biomineralization within ZIF-8. With purified HRP, enzyme@ZIF-8 biocomposites withhigher activity were obtained, while periodate oxidation of the carbohydrate component of bothcommercial HRP and purified HRP-C yields biocomposites with very high activity in acetate bufferthat does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent thefalse high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At thesame time, purification and especially oxidation of the carbohydrate component of enzymes prior tobiomineralization lead to significantly improved activity of the biocomposites.",
publisher = "MDPI",
journal = "Polymers",
title = "The influence of isoenzymes composition and chemical modification on horseradish peroxidase@ZIF-8 bio-composite performance",
volume = "14",
pages = "4834",
doi = "10.3390/polym14224834"
}
Stanišić, M. D., Popović Kokar, N., Ristić, P., Balaž, A. M., Ognjanović, M., Đokić, V. R., Prodanović, R.,& Todorović, T.. (2022). The influence of isoenzymes composition and chemical modification on horseradish peroxidase@ZIF-8 bio-composite performance. in Polymers
MDPI., 14, 4834.
https://doi.org/10.3390/polym14224834
Stanišić MD, Popović Kokar N, Ristić P, Balaž AM, Ognjanović M, Đokić VR, Prodanović R, Todorović T. The influence of isoenzymes composition and chemical modification on horseradish peroxidase@ZIF-8 bio-composite performance. in Polymers. 2022;14:4834.
doi:10.3390/polym14224834 .
Stanišić, Marija D., Popović Kokar, Nikolina, Ristić, Predrag, Balaž, Ana Marija, Ognjanović, Miloš, Đokić, Veljko R., Prodanović, Radivoje, Todorović, Tamara, "The influence of isoenzymes composition and chemical modification on horseradish peroxidase@ZIF-8 bio-composite performance" in Polymers, 14 (2022):4834,
https://doi.org/10.3390/polym14224834 . .
