Electrochemical and EPR investigation of spin probes acetoxymethoxycarbonyl- and carboxy-proxyl

Abstract

Redox properties of one hydrophobic spin probe 3-(acetoxymethoxy)carbonyl-2,2,5,5-tetramethyl pyrrolidine-N-oxyl (AM-CxP) and corresponding water-soluble compound, 3-carboxy-2,2,5,5- tetramethyl-pyrrolidine-N-oxyl (3-CxP) were evaluated using electrochemical and EPR methods. The electrochemical behavior of two spin probes was investigated by cyclic voltammetry in phosphate buffer solution. The voltammograms of both compounds exhibited a single redox couple related to nitroxyl radical. EPR spectroscopy revealed that 3-CxP is less susceptible to reduction by Ascorbate than AM-CxP, nevertheless, both spin probes exhibit a great potential for in vivo applications