Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus
Authors
Anđelković, Boban
Gođevac, Dejan

Stanković, Jovana

Cvetković, Mirjana

Tešević, Vele

Milosavljević, Slobodan

Simić, Katarina

Conference object (Published version)
Metadata
Show full item recordAbstract
A large number of plant metabolites has provided an incomparable chemical source of the pharmaceutical
products. The two major fields of chemical research on biological active small molecules, metabolomics and
natural product discovery, have the similar goals of identifying and characterizing small molecules, either in
their isolated active state (natural product chemistry). Metabolomics generate a profile of small molecules
from plant extracts, which could be directly responsible for bioactivity effects. Using dry-column flash
chromatography enable a rapid and inexpensive method for the very efficient separation of plant extract with
a high resolution. This separation method coupled to NMR and FTIR-based metabolomics is applied to identify
bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical
composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against
Human cervical adenocarcinoma c...ell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the
highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactone were
tested, and their cytotoxic activity were conformed.
Keywords:
plant metabolites / NMR / FTIR / metabolomicsSource:
Book of abstracts - 5th International Caparica Christmas Conference on Sample Treatment (ST 2021), 15th – 18th November 2021, Caparica, Portugal, 2021, 148-148Publisher:
- Portugal : PROTEOMASS Scientific Society
Funding / projects:
Collections
Institution/Community
IHTMTY - CONF AU - Anđelković, Boban AU - Gođevac, Dejan AU - Stanković, Jovana AU - Cvetković, Mirjana AU - Tešević, Vele AU - Milosavljević, Slobodan AU - Simić, Katarina PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5526 AB - A large number of plant metabolites has provided an incomparable chemical source of the pharmaceutical products. The two major fields of chemical research on biological active small molecules, metabolomics and natural product discovery, have the similar goals of identifying and characterizing small molecules, either in their isolated active state (natural product chemistry). Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using dry-column flash chromatography enable a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIR-based metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactone were tested, and their cytotoxic activity were conformed. PB - Portugal : PROTEOMASS Scientific Society C3 - Book of abstracts - 5th International Caparica Christmas Conference on Sample Treatment (ST 2021), 15th – 18th November 2021, Caparica, Portugal T1 - Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus SP - 148 EP - 148 UR - https://hdl.handle.net/21.15107/rcub_cer_5526 ER -
@conference{ author = "Anđelković, Boban and Gođevac, Dejan and Stanković, Jovana and Cvetković, Mirjana and Tešević, Vele and Milosavljević, Slobodan and Simić, Katarina", year = "2021", abstract = "A large number of plant metabolites has provided an incomparable chemical source of the pharmaceutical products. The two major fields of chemical research on biological active small molecules, metabolomics and natural product discovery, have the similar goals of identifying and characterizing small molecules, either in their isolated active state (natural product chemistry). Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using dry-column flash chromatography enable a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIR-based metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactone were tested, and their cytotoxic activity were conformed.", publisher = "Portugal : PROTEOMASS Scientific Society", journal = "Book of abstracts - 5th International Caparica Christmas Conference on Sample Treatment (ST 2021), 15th – 18th November 2021, Caparica, Portugal", title = "Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus", pages = "148-148", url = "https://hdl.handle.net/21.15107/rcub_cer_5526" }
Anđelković, B., Gođevac, D., Stanković, J., Cvetković, M., Tešević, V., Milosavljević, S.,& Simić, K.. (2021). Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus. in Book of abstracts - 5th International Caparica Christmas Conference on Sample Treatment (ST 2021), 15th – 18th November 2021, Caparica, Portugal Portugal : PROTEOMASS Scientific Society., 148-148. https://hdl.handle.net/21.15107/rcub_cer_5526
Anđelković B, Gođevac D, Stanković J, Cvetković M, Tešević V, Milosavljević S, Simić K. Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus. in Book of abstracts - 5th International Caparica Christmas Conference on Sample Treatment (ST 2021), 15th – 18th November 2021, Caparica, Portugal. 2021;:148-148. https://hdl.handle.net/21.15107/rcub_cer_5526 .
Anđelković, Boban, Gođevac, Dejan, Stanković, Jovana, Cvetković, Mirjana, Tešević, Vele, Milosavljević, Slobodan, Simić, Katarina, "Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus" in Book of abstracts - 5th International Caparica Christmas Conference on Sample Treatment (ST 2021), 15th – 18th November 2021, Caparica, Portugal (2021):148-148, https://hdl.handle.net/21.15107/rcub_cer_5526 .