GC-MS based metabolomics study of the resurrection plant Ramonda serbica
Аутори
Gođevac, DejanSimić, Katarina
Ivanović, Stefan
Anđelković, Boban
Jovanović, Živko
Rakić, Tamara
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ramonda serbica represents the remnant of the Tertiary tropical and subtropical flora in Europe and is the rare resurrection plants of Northern Hemisphere temperate zone. The plant remains well-hydrated during spring, late autumn and in winter. In summer and early autumn when plants are subjected to drought, their desiccation tolerance comes into operation and they spend it in anabiosis [1]. In this study, metabolic responses to dehydration and rehydration of R. serbica, were investigated. For this purpose, GC-MS/FID
based metabolomics method was performed. Leaves from the control (well-watered), dehydrated and partially rehydrated plants were sampled. Each leaf was powdered using liquid nitrogen and then freeze-dried. The internal standard (10-undecenoic acid) was added to the dry plant material and then extracted in methanol/water using ultra-sonication. After centrifugation of the mixture, the supernatant was dried and derivatized using two-step procedure involving oximation and si...lylation. GC-MS/FID analysis of each sample was then performed. The metabolites were identified using El-MS spectra. The areas from FID chromatograms were used for multivariate data analysis. Two OPLS-DA models were applied to investigate dehydration and rehydration process. According to high VIP scores, sugars, such as fructose, glucose, galactinol, sucrose, together with glyceric acid, xylonic acid 8-lactone and aspartic acid were found to be the most influential in the OPLS-DA models.
Кључне речи:
Ramonda serbica / gc-ms metabolomics / opls-daИзвор:
Book of abstracts - 4th international conference on natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria, 2019, 189-190Издавач:
- ICNPU-2019
Институција/група
IHTMTY - CONF AU - Gođevac, Dejan AU - Simić, Katarina AU - Ivanović, Stefan AU - Anđelković, Boban AU - Jovanović, Živko AU - Rakić, Tamara PY - 2019 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5524 AB - Ramonda serbica represents the remnant of the Tertiary tropical and subtropical flora in Europe and is the rare resurrection plants of Northern Hemisphere temperate zone. The plant remains well-hydrated during spring, late autumn and in winter. In summer and early autumn when plants are subjected to drought, their desiccation tolerance comes into operation and they spend it in anabiosis [1]. In this study, metabolic responses to dehydration and rehydration of R. serbica, were investigated. For this purpose, GC-MS/FID based metabolomics method was performed. Leaves from the control (well-watered), dehydrated and partially rehydrated plants were sampled. Each leaf was powdered using liquid nitrogen and then freeze-dried. The internal standard (10-undecenoic acid) was added to the dry plant material and then extracted in methanol/water using ultra-sonication. After centrifugation of the mixture, the supernatant was dried and derivatized using two-step procedure involving oximation and silylation. GC-MS/FID analysis of each sample was then performed. The metabolites were identified using El-MS spectra. The areas from FID chromatograms were used for multivariate data analysis. Two OPLS-DA models were applied to investigate dehydration and rehydration process. According to high VIP scores, sugars, such as fructose, glucose, galactinol, sucrose, together with glyceric acid, xylonic acid 8-lactone and aspartic acid were found to be the most influential in the OPLS-DA models. PB - ICNPU-2019 C3 - Book of abstracts - 4th international conference on natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria T1 - GC-MS based metabolomics study of the resurrection plant Ramonda serbica SP - 189 EP - 190 UR - https://hdl.handle.net/21.15107/rcub_cer_5524 ER -
@conference{ author = "Gođevac, Dejan and Simić, Katarina and Ivanović, Stefan and Anđelković, Boban and Jovanović, Živko and Rakić, Tamara", year = "2019", abstract = "Ramonda serbica represents the remnant of the Tertiary tropical and subtropical flora in Europe and is the rare resurrection plants of Northern Hemisphere temperate zone. The plant remains well-hydrated during spring, late autumn and in winter. In summer and early autumn when plants are subjected to drought, their desiccation tolerance comes into operation and they spend it in anabiosis [1]. In this study, metabolic responses to dehydration and rehydration of R. serbica, were investigated. For this purpose, GC-MS/FID based metabolomics method was performed. Leaves from the control (well-watered), dehydrated and partially rehydrated plants were sampled. Each leaf was powdered using liquid nitrogen and then freeze-dried. The internal standard (10-undecenoic acid) was added to the dry plant material and then extracted in methanol/water using ultra-sonication. After centrifugation of the mixture, the supernatant was dried and derivatized using two-step procedure involving oximation and silylation. GC-MS/FID analysis of each sample was then performed. The metabolites were identified using El-MS spectra. The areas from FID chromatograms were used for multivariate data analysis. Two OPLS-DA models were applied to investigate dehydration and rehydration process. According to high VIP scores, sugars, such as fructose, glucose, galactinol, sucrose, together with glyceric acid, xylonic acid 8-lactone and aspartic acid were found to be the most influential in the OPLS-DA models.", publisher = "ICNPU-2019", journal = "Book of abstracts - 4th international conference on natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria", title = "GC-MS based metabolomics study of the resurrection plant Ramonda serbica", pages = "189-190", url = "https://hdl.handle.net/21.15107/rcub_cer_5524" }
Gođevac, D., Simić, K., Ivanović, S., Anđelković, B., Jovanović, Ž.,& Rakić, T.. (2019). GC-MS based metabolomics study of the resurrection plant Ramonda serbica. in Book of abstracts - 4th international conference on natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria ICNPU-2019., 189-190. https://hdl.handle.net/21.15107/rcub_cer_5524
Gođevac D, Simić K, Ivanović S, Anđelković B, Jovanović Ž, Rakić T. GC-MS based metabolomics study of the resurrection plant Ramonda serbica. in Book of abstracts - 4th international conference on natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria. 2019;:189-190. https://hdl.handle.net/21.15107/rcub_cer_5524 .
Gođevac, Dejan, Simić, Katarina, Ivanović, Stefan, Anđelković, Boban, Jovanović, Živko, Rakić, Tamara, "GC-MS based metabolomics study of the resurrection plant Ramonda serbica" in Book of abstracts - 4th international conference on natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria (2019):189-190, https://hdl.handle.net/21.15107/rcub_cer_5524 .