This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential bene...fits of polyphenolic compounds from raspberry seeds as efficient antioxidants.
TY - JOUR
AU - Gođevac, Dejan
AU - Tešević, Vele
AU - Vajs, Vlatka
AU - Milosavljević, Slobodan
AU - Stanković, Miroslava
PY - 2009
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/526
AB - This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential benefits of polyphenolic compounds from raspberry seeds as efficient antioxidants.
PB - Pergamon-Elsevier Science Ltd, Oxford
T2 - Food and Chemical Toxicology
T1 - Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes
VL - 47
IS - 11
SP - 2853
EP - 2859
DO - 10.1016/j.fct.2009.09.006
ER -
@article{
author = "Gođevac, Dejan and Tešević, Vele and Vajs, Vlatka and Milosavljević, Slobodan and Stanković, Miroslava",
year = "2009",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/526",
abstract = "This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential benefits of polyphenolic compounds from raspberry seeds as efficient antioxidants.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes",
volume = "47",
number = "11",
pages = "2853-2859",
doi = "10.1016/j.fct.2009.09.006"
}
Gođevac D, Tešević V, Vajs V, Milosavljević S, Stanković M. Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes. Food and Chemical Toxicology. 2009;47(11):2853-2859
Gođevac, D., Tešević, V., Vajs, V., Milosavljević, S.,& Stanković, M. (2009). Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes.
Food and Chemical ToxicologyPergamon-Elsevier Science Ltd, Oxford., 47(11), 2853-2859.
https://doi.org/10.1016/j.fct.2009.09.006
Gođevac Dejan, Tešević Vele, Vajs Vlatka, Milosavljević Slobodan, Stanković Miroslava, "Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes" 47, no. 11 (2009):2853-2859,
https://doi.org/10.1016/j.fct.2009.09.006 .
