Evaluation of antitumor potential of Cu(II) complex withhydrazone of 2-acetylthiazole and Girard’s T reagent
Authors
Stevanović, Nevena
Jevtović, Mima

Mitić, Dragana

Matić, Ivana Z.

Crnogorac, Marija

Vujčić, Miroslava

Sladić, Dušan

Čobeljić, Božidar

Anđelković, Katarina

Article (Published version)
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In this paper, the previously synthesized Cu(II) complex ([CuL1(N3) (CH3OH)]BF4) with N,N,N-trimethyl-2-oxo-2-(2-(1-(thiazol-2-yl)ethylidene)hydrazinyl)ethan-1-aminium chloride, has been characterized and its biological activity has been studied in detail. The Cu(II) complex consists of ligand coordinated in a deprotonated, formally neutral zwitter-ionic form, via NNO atoms, one azido ligand and one methanol molecule. The Cu(II) complex was selected due to results of the cytotoxic activity, the brine shrimp test and DPPH radical scavenging activity, which were previously performed. The effects of Cu(II) complex on cell cycle phase distribution of cervical adenocarcinoma HeLa cells were investigated in order to examine the mechanisms of its anticancer activity. The measurement of intracellular ROS levels in HeLa and HaCaT cell lines were evaluated in order to explore their possible generation and the role in cytotoxic activity. The possible anti-invasive and anti-angiogenic properties o...f Cu(II) complex were evaluated. DNA binding experiments, including fluorescence displacement study and DNA cleavage experiments, were performed in order to obtain information on the type of DNA-metal complex interactions. © 2022 Serbian Chemical Society. All rights reserved.
Keywords:
cell cycle analysis / gene expression analyses / N-acylhydrazone / UV-Vis measurementsSource:
Journal of the Serbian Chemical Society, 2022, 87, 2, 181-192Publisher:
- Beograd : Srpsko hemijsko društvo
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200043 (Institute of Oncology and Radiology of Serbia, Belgrade) (RS-200043)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-200026)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200168 (University of Belgrade, Faculty of Chemistry) (RS-200168)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200288 (Innovation Center of the Faculty of Chemistry) (RS-200288)
DOI: 10.2298/JSC211203114S
ISSN: 0352-5139
WoS: 000751730600001
Scopus: 2-s2.0-85126719693
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IHTMTY - JOUR AU - Stevanović, Nevena AU - Jevtović, Mima AU - Mitić, Dragana AU - Matić, Ivana Z. AU - Crnogorac, Marija AU - Vujčić, Miroslava AU - Sladić, Dušan AU - Čobeljić, Božidar AU - Anđelković, Katarina PY - 2022 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5230 AB - In this paper, the previously synthesized Cu(II) complex ([CuL1(N3) (CH3OH)]BF4) with N,N,N-trimethyl-2-oxo-2-(2-(1-(thiazol-2-yl)ethylidene)hydrazinyl)ethan-1-aminium chloride, has been characterized and its biological activity has been studied in detail. The Cu(II) complex consists of ligand coordinated in a deprotonated, formally neutral zwitter-ionic form, via NNO atoms, one azido ligand and one methanol molecule. The Cu(II) complex was selected due to results of the cytotoxic activity, the brine shrimp test and DPPH radical scavenging activity, which were previously performed. The effects of Cu(II) complex on cell cycle phase distribution of cervical adenocarcinoma HeLa cells were investigated in order to examine the mechanisms of its anticancer activity. The measurement of intracellular ROS levels in HeLa and HaCaT cell lines were evaluated in order to explore their possible generation and the role in cytotoxic activity. The possible anti-invasive and anti-angiogenic properties of Cu(II) complex were evaluated. DNA binding experiments, including fluorescence displacement study and DNA cleavage experiments, were performed in order to obtain information on the type of DNA-metal complex interactions. © 2022 Serbian Chemical Society. All rights reserved. PB - Beograd : Srpsko hemijsko društvo T2 - Journal of the Serbian Chemical Society T1 - Evaluation of antitumor potential of Cu(II) complex withhydrazone of 2-acetylthiazole and Girard’s T reagent VL - 87 IS - 2 SP - 181 EP - 192 DO - 10.2298/JSC211203114S ER -
@article{ author = "Stevanović, Nevena and Jevtović, Mima and Mitić, Dragana and Matić, Ivana Z. and Crnogorac, Marija and Vujčić, Miroslava and Sladić, Dušan and Čobeljić, Božidar and Anđelković, Katarina", year = "2022", abstract = "In this paper, the previously synthesized Cu(II) complex ([CuL1(N3) (CH3OH)]BF4) with N,N,N-trimethyl-2-oxo-2-(2-(1-(thiazol-2-yl)ethylidene)hydrazinyl)ethan-1-aminium chloride, has been characterized and its biological activity has been studied in detail. The Cu(II) complex consists of ligand coordinated in a deprotonated, formally neutral zwitter-ionic form, via NNO atoms, one azido ligand and one methanol molecule. The Cu(II) complex was selected due to results of the cytotoxic activity, the brine shrimp test and DPPH radical scavenging activity, which were previously performed. The effects of Cu(II) complex on cell cycle phase distribution of cervical adenocarcinoma HeLa cells were investigated in order to examine the mechanisms of its anticancer activity. The measurement of intracellular ROS levels in HeLa and HaCaT cell lines were evaluated in order to explore their possible generation and the role in cytotoxic activity. The possible anti-invasive and anti-angiogenic properties of Cu(II) complex were evaluated. DNA binding experiments, including fluorescence displacement study and DNA cleavage experiments, were performed in order to obtain information on the type of DNA-metal complex interactions. © 2022 Serbian Chemical Society. All rights reserved.", publisher = "Beograd : Srpsko hemijsko društvo", journal = "Journal of the Serbian Chemical Society", title = "Evaluation of antitumor potential of Cu(II) complex withhydrazone of 2-acetylthiazole and Girard’s T reagent", volume = "87", number = "2", pages = "181-192", doi = "10.2298/JSC211203114S" }
Stevanović, N., Jevtović, M., Mitić, D., Matić, I. Z., Crnogorac, M., Vujčić, M., Sladić, D., Čobeljić, B.,& Anđelković, K.. (2022). Evaluation of antitumor potential of Cu(II) complex withhydrazone of 2-acetylthiazole and Girard’s T reagent. in Journal of the Serbian Chemical Society Beograd : Srpsko hemijsko društvo., 87(2), 181-192. https://doi.org/10.2298/JSC211203114S
Stevanović N, Jevtović M, Mitić D, Matić IZ, Crnogorac M, Vujčić M, Sladić D, Čobeljić B, Anđelković K. Evaluation of antitumor potential of Cu(II) complex withhydrazone of 2-acetylthiazole and Girard’s T reagent. in Journal of the Serbian Chemical Society. 2022;87(2):181-192. doi:10.2298/JSC211203114S .
Stevanović, Nevena, Jevtović, Mima, Mitić, Dragana, Matić, Ivana Z., Crnogorac, Marija, Vujčić, Miroslava, Sladić, Dušan, Čobeljić, Božidar, Anđelković, Katarina, "Evaluation of antitumor potential of Cu(II) complex withhydrazone of 2-acetylthiazole and Girard’s T reagent" in Journal of the Serbian Chemical Society, 87, no. 2 (2022):181-192, https://doi.org/10.2298/JSC211203114S . .