A study of the flexibility of the carbon catabolic pathways of extremophilic P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon sources by multi omics analytical platform
Само за регистроване кориснике
2022
Аутори
Medić, AnaHuttmann, Nico
Lješević, Marija
Risha, Yousef
Berezovski, Maxim
Minić, Zoran
Karadžić, Ivanka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Ent...ner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.
Кључне речи:
Pseudomonas aeruginosa / Carbon metabolism / Benzoate / Proteome / Metabolomics / BiodegradationИзвор:
Microbiological Research, 2022, 259, 126998-Издавач:
- Elsevier
Финансирање / пројекти:
- Симултана биоремедијација и соилификација деградираних простора, за очување природних ресурса биолошки активних супстанци и развој и производњу биоматеријала и дијететских производа (RS-43004)
- Геохемијска испитивања седиментних стена - фосилна горива и загађивачи животне средине (RS-176006)
Напомена:
- The peer-reviewed version: https://cer.ihtm.bg.ac.rs/handle/123456789/5548
Повезане информације:
- Друга верзија
https://cer.ihtm.bg.ac.rs/handle/123456789/5548
DOI: 10.1016/j.micres.2022.126998
ISSN: 0944-5013
PubMed: 35276454
WoS: 00079326340000
Scopus: 2-s2.0-85125714802
Институција/група
IHTMTY - JOUR AU - Medić, Ana AU - Huttmann, Nico AU - Lješević, Marija AU - Risha, Yousef AU - Berezovski, Maxim AU - Minić, Zoran AU - Karadžić, Ivanka PY - 2022 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5190 AB - Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application. PB - Elsevier T2 - Microbiological Research T1 - A study of the flexibility of the carbon catabolic pathways of extremophilic P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon sources by multi omics analytical platform VL - 259 SP - 126998 DO - 10.1016/j.micres.2022.126998 ER -
@article{ author = "Medić, Ana and Huttmann, Nico and Lješević, Marija and Risha, Yousef and Berezovski, Maxim and Minić, Zoran and Karadžić, Ivanka", year = "2022", abstract = "Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.", publisher = "Elsevier", journal = "Microbiological Research", title = "A study of the flexibility of the carbon catabolic pathways of extremophilic P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon sources by multi omics analytical platform", volume = "259", pages = "126998", doi = "10.1016/j.micres.2022.126998" }
Medić, A., Huttmann, N., Lješević, M., Risha, Y., Berezovski, M., Minić, Z.,& Karadžić, I.. (2022). A study of the flexibility of the carbon catabolic pathways of extremophilic P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon sources by multi omics analytical platform. in Microbiological Research Elsevier., 259, 126998. https://doi.org/10.1016/j.micres.2022.126998
Medić A, Huttmann N, Lješević M, Risha Y, Berezovski M, Minić Z, Karadžić I. A study of the flexibility of the carbon catabolic pathways of extremophilic P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon sources by multi omics analytical platform. in Microbiological Research. 2022;259:126998. doi:10.1016/j.micres.2022.126998 .
Medić, Ana, Huttmann, Nico, Lješević, Marija, Risha, Yousef, Berezovski, Maxim, Minić, Zoran, Karadžić, Ivanka, "A study of the flexibility of the carbon catabolic pathways of extremophilic P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon sources by multi omics analytical platform" in Microbiological Research, 259 (2022):126998, https://doi.org/10.1016/j.micres.2022.126998 . .