β-D-glukan iz pekarskog kvasca: antioksidativne i bifidogene osobine

Abstract

Ćelijski zid pekarskog kvasca je glavni izvor nesvarljivog polisaharida beta-D-glukana Beta-D-glukan je fiziološki aktivno jedinjenje (opšte poznato kao modulator biološkog odgovora), koji aktivira imuni odgovor domaćina protiv bakterijske, virusne, gljivične i parazitske infekcije, kao i neoplazija. Cilj našeg istraživanja je ispitivanje bifidogenog i antioksidativnog potencijala (1->3),(1->6)-beta-D-glukana izolovanog iz pekarskog kvasca, kao novog prebiotskog dodatka infant formulama. Ukupan broj bifidobakterija nakon 48 h inkubacije u infant formuli sa dodatkom 0,1% (m /V) beta-D-glukana (čistoće 99,54%) bio je značajno viši u odnosu na zrelo majčino mleko, infant formulu sa dodatkom inulina ili infant formulu bez prebiotika, kao referentne supstrate. Promene broja bifidobakterija pra ene su promenama suve biomase, ukupnih bakterijski generisanih kiselina i pH. beta-D-glukan najveće čistoće nema antioksidativnu aktivnost, dok prečišćeni ekstrakti glukana (93,15%, 75,54% i 49,30%) uklanjaju hidroksil radikale. Na osnovu bifidogenog efekta možemo da zaključimo da je beta-D-glukan iz kvasca dobar kandidat kao novi prebiotik za dopunu infant formula.

The cell wall of baker’s yeast is a major source of nondigestible polysaccharide beta-glucan ((1->3),(1->6)-beta-D-glucan). Baker’s yeast beta-glucan is a physiologically active compound (generally named “biological response modifier”) and is a broad-spectrum enhancer of host defense against bacterial, viral, fungal and parasitic infections, as well as neoplasia. The aim of our study was to investigate the bifidogenic and antioxidative potential of (1->3),(1->6)-beta-D-glucan isolated from the baker’s yeast (Saccharomyces cerevisiae) in relation to digestibility and purity, as a new infant formula prebiotic supplement. The total number of bifidobacteria after 48 h of incubation in the substrate composed of infant formula supplemented with 0.1 % (m/v) beta-D-glucan (purity 99.54 %) was significantly higher than in mature breast milk, infant formula supplemented with inuline or infant formula without added prebiotic, which were used as reference substrates. Changes in the number of bifidobacteria were followed by the changes in dry biomass, total bacteria-generated organic acids and pH. In contrast, the purest beta-D-glucan did not show any antioxidative activity, while partially purified glucan extracts (93.15%, 75.54% and 49.30%) scavenged hydroxyl radicals. Regarding to digestibility and bifidogenic efficacy Saccharomyces cerevisiae beta-D-glucan could be a candidate as a new infant formula prebiotic supplement.