Ispitivanje tolerancije soja Pseudomonas aeruginosa san ai na živu, kadmijum i bakar

Abstract

Zagadenje zivotne okoline teskim metalima je ozbiljan problem savremenog sveta. Među metalima koji su cesti zagadjivaci su: bakar, kadmijum i ziva. Cilj ovog rada je bilo ispitivanje osetljivosti soja Pseudomonas aeruginosa san ai na pomenute teske metale. Minimale inhibitome koncentracije kadmijuma i bakra od 2 i 4 mM, redom, pokazale su da je soj otporan na ova dva metala. MIK za zivu je bila najniza testirana koncentracija metala od 0,5 mM. Dobijene vrednosti MIK-aza kadmijum i bakar stavljaju soj P. aeruginosa san ai u grupu bakterija koje ispoljavaju visokutoleranciju prema ovim teskim metalima. Utvrdeno je daje ziva toksicna za soj. lspitivanja mehanizama detoksikacije soja P. aeruginosa san ai u cilju potencijalne primene u procesima bioremedijacije su u toku.

The pollution of the environment with toxic heavy metals is spreading throughout the world along with industrial progress. Among the common heavy metals used and the more widespread contaminants of the environment are mercury, cadmium and copper. The present research was performed in order to screen tolerance of strain Pseudomonas aeruginosa san ai to these heavy metals Study showed that P. aeruginosa san ai is tolerant to cadmium and copper with MIC's of 2 and4 mM, respectively. MIC for mercury was the lowest tested concentration of 0.5 roM. This suggests that P. aeruginosa san ai has a large potential for bioremediation of environments contaminated with cadmium and copper and that mercury is toxic for the strain. In order to improve possible application of strain, P. aeruginosa san ai in processes of bioremediation the study of mechanisams of metal uptake are underway.