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MALDI-TOF-MS Characterization of Environmental Bacterial Isolates

MALDI-TOF-MS karakterizacija bakterija izolovanih iz životne sredine

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2013
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Authors
Beškoski, Vladimir
Mattinen, Maija-Lisa
Milić, Jelena
Avdalović, Jelena
Ilić, Mila
Miletić, Srđan
Gojgić-Cvijović, Gordana
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Abstract
Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000 Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database.
Keywords:
microbiological methods / MALDI-TOF-MS
Source:
6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013, 2013, 73, 72-
Publisher:
  • Belgrade : Serbian Chemical Society

ISBN: 978-86-7132-052-8

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_cer_5050
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/5050
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - CONF
AU  - Beškoski, Vladimir
AU  - Mattinen, Maija-Lisa
AU  - Milić, Jelena
AU  - Avdalović, Jelena
AU  - Ilić, Mila
AU  - Miletić, Srđan
AU  - Gojgić-Cvijović, Gordana
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5050
AB  - Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and 
 negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000 
Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database.
PB  - Belgrade : Serbian Chemical Society
C3  - 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013
T1  - MALDI-TOF-MS Characterization of Environmental Bacterial Isolates
T1  - MALDI-TOF-MS karakterizacija bakterija  izolovanih iz životne sredine
VL  - 73
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_cer_5050
ER  - 
@conference{
author = "Beškoski, Vladimir and Mattinen, Maija-Lisa and Milić, Jelena and Avdalović, Jelena and Ilić, Mila and Miletić, Srđan and Gojgić-Cvijović, Gordana",
year = "2013",
abstract = "Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and 
 negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000 
Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013",
title = "MALDI-TOF-MS Characterization of Environmental Bacterial Isolates, MALDI-TOF-MS karakterizacija bakterija  izolovanih iz životne sredine",
volume = "73",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_cer_5050"
}
Beškoski, V., Mattinen, M., Milić, J., Avdalović, J., Ilić, M., Miletić, S.,& Gojgić-Cvijović, G.. (2013). MALDI-TOF-MS Characterization of Environmental Bacterial Isolates. in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013
Belgrade : Serbian Chemical Society., 73, 72.
https://hdl.handle.net/21.15107/rcub_cer_5050
Beškoski V, Mattinen M, Milić J, Avdalović J, Ilić M, Miletić S, Gojgić-Cvijović G. MALDI-TOF-MS Characterization of Environmental Bacterial Isolates. in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013. 2013;73:72.
https://hdl.handle.net/21.15107/rcub_cer_5050 .
Beškoski, Vladimir, Mattinen, Maija-Lisa, Milić, Jelena, Avdalović, Jelena, Ilić, Mila, Miletić, Srđan, Gojgić-Cvijović, Gordana, "MALDI-TOF-MS Characterization of Environmental Bacterial Isolates" in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013, 73 (2013):72,
https://hdl.handle.net/21.15107/rcub_cer_5050 .

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