MALDI-TOF-MS Characterization of Environmental Bacterial Isolates
MALDI-TOF-MS karakterizacija bakterija izolovanih iz životne sredine
Authors
Beškoski, Vladimir
Mattinen, Maija-Lisa
Milić, Jelena

Avdalović, Jelena

Ilić, Mila

Miletić, Srđan

Gojgić-Cvijović, Gordana

Conference object (Published version)
Metadata
Show full item recordAbstract
Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and
negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000
Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database.
Keywords:
microbiological methods / MALDI-TOF-MSSource:
6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013, 2013, 73, 72-Publisher:
- Belgrade : Serbian Chemical Society
Collections
Institution/Community
IHTMTY - CONF AU - Beškoski, Vladimir AU - Mattinen, Maija-Lisa AU - Milić, Jelena AU - Avdalović, Jelena AU - Ilić, Mila AU - Miletić, Srđan AU - Gojgić-Cvijović, Gordana PY - 2013 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5050 AB - Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000 Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database. PB - Belgrade : Serbian Chemical Society C3 - 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013 T1 - MALDI-TOF-MS Characterization of Environmental Bacterial Isolates T1 - MALDI-TOF-MS karakterizacija bakterija izolovanih iz životne sredine VL - 73 SP - 72 UR - https://hdl.handle.net/21.15107/rcub_cer_5050 ER -
@conference{ author = "Beškoski, Vladimir and Mattinen, Maija-Lisa and Milić, Jelena and Avdalović, Jelena and Ilić, Mila and Miletić, Srđan and Gojgić-Cvijović, Gordana", year = "2013", abstract = "Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000 Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database.", publisher = "Belgrade : Serbian Chemical Society", journal = "6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013", title = "MALDI-TOF-MS Characterization of Environmental Bacterial Isolates, MALDI-TOF-MS karakterizacija bakterija izolovanih iz životne sredine", volume = "73", pages = "72", url = "https://hdl.handle.net/21.15107/rcub_cer_5050" }
Beškoski, V., Mattinen, M., Milić, J., Avdalović, J., Ilić, M., Miletić, S.,& Gojgić-Cvijović, G.. (2013). MALDI-TOF-MS Characterization of Environmental Bacterial Isolates. in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013 Belgrade : Serbian Chemical Society., 73, 72. https://hdl.handle.net/21.15107/rcub_cer_5050
Beškoski V, Mattinen M, Milić J, Avdalović J, Ilić M, Miletić S, Gojgić-Cvijović G. MALDI-TOF-MS Characterization of Environmental Bacterial Isolates. in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013. 2013;73:72. https://hdl.handle.net/21.15107/rcub_cer_5050 .
Beškoski, Vladimir, Mattinen, Maija-Lisa, Milić, Jelena, Avdalović, Jelena, Ilić, Mila, Miletić, Srđan, Gojgić-Cvijović, Gordana, "MALDI-TOF-MS Characterization of Environmental Bacterial Isolates" in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013, 73 (2013):72, https://hdl.handle.net/21.15107/rcub_cer_5050 .