MALDI-TOF-MS Characterization of Environmental Bacterial Isolates
MALDI-TOF-MS karakterizacija bakterija izolovanih iz životne sredine
Аутори
Beškoski, VladimirMattinen, Maija-Lisa
Milić, Jelena
Avdalović, Jelena
Ilić, Mila
Miletić, Srđan
Gojgić-Cvijović, Gordana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and
negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000
Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database.
Кључне речи:
microbiological methods / MALDI-TOF-MSИзвор:
6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013, 2013, 73, 72-Издавач:
- Belgrade : Serbian Chemical Society
Институција/група
IHTMTY - CONF AU - Beškoski, Vladimir AU - Mattinen, Maija-Lisa AU - Milić, Jelena AU - Avdalović, Jelena AU - Ilić, Mila AU - Miletić, Srđan AU - Gojgić-Cvijović, Gordana PY - 2013 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5050 AB - Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000 Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database. PB - Belgrade : Serbian Chemical Society C3 - 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013 T1 - MALDI-TOF-MS Characterization of Environmental Bacterial Isolates T1 - MALDI-TOF-MS karakterizacija bakterija izolovanih iz životne sredine VL - 73 SP - 72 UR - https://hdl.handle.net/21.15107/rcub_cer_5050 ER -
@conference{ author = "Beškoski, Vladimir and Mattinen, Maija-Lisa and Milić, Jelena and Avdalović, Jelena and Ilić, Mila and Miletić, Srđan and Gojgić-Cvijović, Gordana", year = "2013", abstract = "Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000 Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database.", publisher = "Belgrade : Serbian Chemical Society", journal = "6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013", title = "MALDI-TOF-MS Characterization of Environmental Bacterial Isolates, MALDI-TOF-MS karakterizacija bakterija izolovanih iz životne sredine", volume = "73", pages = "72", url = "https://hdl.handle.net/21.15107/rcub_cer_5050" }
Beškoski, V., Mattinen, M., Milić, J., Avdalović, J., Ilić, M., Miletić, S.,& Gojgić-Cvijović, G.. (2013). MALDI-TOF-MS Characterization of Environmental Bacterial Isolates. in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013 Belgrade : Serbian Chemical Society., 73, 72. https://hdl.handle.net/21.15107/rcub_cer_5050
Beškoski V, Mattinen M, Milić J, Avdalović J, Ilić M, Miletić S, Gojgić-Cvijović G. MALDI-TOF-MS Characterization of Environmental Bacterial Isolates. in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013. 2013;73:72. https://hdl.handle.net/21.15107/rcub_cer_5050 .
Beškoski, Vladimir, Mattinen, Maija-Lisa, Milić, Jelena, Avdalović, Jelena, Ilić, Mila, Miletić, Srđan, Gojgić-Cvijović, Gordana, "MALDI-TOF-MS Characterization of Environmental Bacterial Isolates" in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013, 73 (2013):72, https://hdl.handle.net/21.15107/rcub_cer_5050 .