Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and
negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000
Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database.
Keywords:
microbiological methods / MALDI-TOF-MS
Source:
6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013, 2013, 73, 72-
TY - CONF
AU - Beškoski, Vladimir
AU - Mattinen, Maija-Lisa
AU - Milić, Jelena
AU - Avdalović, Jelena
AU - Ilić, Mila
AU - Miletić, Srđan
AU - Gojgić-Cvijović, Gordana
PY - 2013
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/5050
AB - Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and
negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000
Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database.
PB - Belgrade : Serbian Chemical Society
C3 - 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013
T1 - MALDI-TOF-MS Characterization of Environmental Bacterial Isolates
T1 - MALDI-TOF-MS karakterizacija bakterija izolovanih iz životne sredine
VL - 73
SP - 72
UR - https://hdl.handle.net/21.15107/rcub_cer_5050
ER -
@conference{
author = "Beškoski, Vladimir and Mattinen, Maija-Lisa and Milić, Jelena and Avdalović, Jelena and Ilić, Mila and Miletić, Srđan and Gojgić-Cvijović, Gordana",
year = "2013",
abstract = "Various traditional and modern microbiological methods are available for the analysis and characterization of pure bacterial cultures. However, for some of them sample preparation can be very time-consuming. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has the advantage of short measuring time, fast sample preparation and
negligible sample consumption. With the utilization of MALDI-TOF MS biomolecules, such as proteins, peptides, oligosaccharides and oligonucleotides in the range of 400 and up to 350,000
Da, can be analysed within a few seconds. Recently, determination of the bacterial samples as intact (whole) cells and also bacterial extracts has been developed. Ten bacterial environmental isolates were characterized using 16SrRNA and subsequently MALDI-TOF-MS, with the use of the Maldi Biotyper database.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013",
title = "MALDI-TOF-MS Characterization of Environmental Bacterial Isolates, MALDI-TOF-MS karakterizacija bakterija izolovanih iz životne sredine",
volume = "73",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_cer_5050"
}
Beškoski, V., Mattinen, M., Milić, J., Avdalović, J., Ilić, M., Miletić, S.,& Gojgić-Cvijović, G.. (2013). MALDI-TOF-MS Characterization of Environmental Bacterial Isolates. in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013
Belgrade : Serbian Chemical Society., 73, 72.
https://hdl.handle.net/21.15107/rcub_cer_5050
Beškoski V, Mattinen M, Milić J, Avdalović J, Ilić M, Miletić S, Gojgić-Cvijović G. MALDI-TOF-MS Characterization of Environmental Bacterial Isolates. in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013. 2013;73:72.
https://hdl.handle.net/21.15107/rcub_cer_5050 .
Beškoski, Vladimir, Mattinen, Maija-Lisa, Milić, Jelena, Avdalović, Jelena, Ilić, Mila, Miletić, Srđan, Gojgić-Cvijović, Gordana, "MALDI-TOF-MS Characterization of Environmental Bacterial Isolates" in 6. Simpozijum Hemija i zaštita životne sredine »Envirochem 2013«, Knjiga izvoda, Vršac 21-24. maj 2013, 73 (2013):72,
https://hdl.handle.net/21.15107/rcub_cer_5050 .
