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Synthesis of chromogenic substrate for screening of pullulan-degrading microorganisms

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2014
bitstream_21508.pdf (1.548Mb)
Authors
Jakovljević, Dragica
Nikolić, J.
Djurić, Aleksandra
Gojgić-Cvijović, Gordana
Beškoski, Vladimir
Vrvić, Miroslav
Conference object (Published version)
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Abstract
A chromogenic substrate was obtained by coupling azo dye Congo red to microbial polysaccharide pullulan. The synthesized product was characterized by spectral techniques and elemental analysis. Results showed that dye-labelled glycan can be successfully used for the screening of microorganisms possessing enzymes that selectively catalyze the cleavage of glycosidic linkages characteristic for this polysaccharide.
Keywords:
pullulan / glycan / polysaccharides / UV-VIS / NMR
Source:
Proceedings - 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry „Physical Chemistry 2014“, Belgrade, September 22-26 2014, 2014, 2, 514-517
Publisher:
  • Belgrade : Society of Physical Chemists of Serbia
Funding / projects:
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)

ISBN: 978-86-82475-30-9

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_cer_4978
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/4978
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - CONF
AU  - Jakovljević, Dragica
AU  - Nikolić, J.
AU  - Djurić, Aleksandra
AU  - Gojgić-Cvijović, Gordana
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4978
AB  - A chromogenic substrate was obtained by coupling azo dye Congo red to microbial polysaccharide pullulan. The synthesized product was characterized by spectral techniques and elemental analysis. Results showed that dye-labelled glycan can be successfully used for the screening of microorganisms possessing enzymes that selectively catalyze the cleavage of glycosidic linkages characteristic for this polysaccharide.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - Proceedings - 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry „Physical Chemistry 2014“, Belgrade, September 22-26 2014
T1  - Synthesis of chromogenic substrate for screening of pullulan-degrading microorganisms
VL  - 2
SP  - 514
EP  - 517
UR  - https://hdl.handle.net/21.15107/rcub_cer_4978
ER  - 
@conference{
author = "Jakovljević, Dragica and Nikolić, J. and Djurić, Aleksandra and Gojgić-Cvijović, Gordana and Beškoski, Vladimir and Vrvić, Miroslav",
year = "2014",
abstract = "A chromogenic substrate was obtained by coupling azo dye Congo red to microbial polysaccharide pullulan. The synthesized product was characterized by spectral techniques and elemental analysis. Results showed that dye-labelled glycan can be successfully used for the screening of microorganisms possessing enzymes that selectively catalyze the cleavage of glycosidic linkages characteristic for this polysaccharide.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "Proceedings - 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry „Physical Chemistry 2014“, Belgrade, September 22-26 2014",
title = "Synthesis of chromogenic substrate for screening of pullulan-degrading microorganisms",
volume = "2",
pages = "514-517",
url = "https://hdl.handle.net/21.15107/rcub_cer_4978"
}
Jakovljević, D., Nikolić, J., Djurić, A., Gojgić-Cvijović, G., Beškoski, V.,& Vrvić, M.. (2014). Synthesis of chromogenic substrate for screening of pullulan-degrading microorganisms. in Proceedings - 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry „Physical Chemistry 2014“, Belgrade, September 22-26 2014
Belgrade : Society of Physical Chemists of Serbia., 2, 514-517.
https://hdl.handle.net/21.15107/rcub_cer_4978
Jakovljević D, Nikolić J, Djurić A, Gojgić-Cvijović G, Beškoski V, Vrvić M. Synthesis of chromogenic substrate for screening of pullulan-degrading microorganisms. in Proceedings - 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry „Physical Chemistry 2014“, Belgrade, September 22-26 2014. 2014;2:514-517.
https://hdl.handle.net/21.15107/rcub_cer_4978 .
Jakovljević, Dragica, Nikolić, J., Djurić, Aleksandra, Gojgić-Cvijović, Gordana, Beškoski, Vladimir, Vrvić, Miroslav, "Synthesis of chromogenic substrate for screening of pullulan-degrading microorganisms" in Proceedings - 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry „Physical Chemistry 2014“, Belgrade, September 22-26 2014, 2 (2014):514-517,
https://hdl.handle.net/21.15107/rcub_cer_4978 .

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