Chemical modification of glycoproteins’ carbohydrate moiety as a general strategy for the synthesis of efficient biocatalysts by biomimetic mineralization: The case of glucose oxidase
Authors
Stanišić, Marija D.Popović Kokar, Nikolina
Ristić, Predrag

Balaž, Ana Marija

Senćanski, Milan

Ognanović, Miloš
Đokić, Veljko R.

Prodanović, Radivoje

Todorović, Tamara

Article (Published version)
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Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to en-capsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein’s surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule, from −10.2 to −36.9 mV, as shown by zeta potential measurements and native PAGE electrophoresis. Biomineralization exp...eriments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Periodate oxidation of carbohydrate components for glycoproteins can serve as a facile and general method for facilitating the biomimetic mineralization of other industrially relevant glycoproteins.
Keywords:
Biocatalysts / Biocomposites / Biomimetic mineralization / Metal–organic frameworks / ZIF-8Source:
Polymers, 2021, 13, 22, 2-12Publisher:
- MDPI
Funding / projects:
DOI: 10.3390/polym13223875
ISSN: 2073-4360
PubMed: 34833174
WoS: 000727517900001
Scopus: 2-s2.0-85119301454
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IHTMTY - JOUR AU - Stanišić, Marija D. AU - Popović Kokar, Nikolina AU - Ristić, Predrag AU - Balaž, Ana Marija AU - Senćanski, Milan AU - Ognanović, Miloš AU - Đokić, Veljko R. AU - Prodanović, Radivoje AU - Todorović, Tamara PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4895 AB - Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to en-capsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein’s surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule, from −10.2 to −36.9 mV, as shown by zeta potential measurements and native PAGE electrophoresis. Biomineralization experiments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Periodate oxidation of carbohydrate components for glycoproteins can serve as a facile and general method for facilitating the biomimetic mineralization of other industrially relevant glycoproteins. PB - MDPI T2 - Polymers T1 - Chemical modification of glycoproteins’ carbohydrate moiety as a general strategy for the synthesis of efficient biocatalysts by biomimetic mineralization: The case of glucose oxidase VL - 13 IS - 22 SP - 2 EP - 12 DO - 10.3390/polym13223875 ER -
@article{ author = "Stanišić, Marija D. and Popović Kokar, Nikolina and Ristić, Predrag and Balaž, Ana Marija and Senćanski, Milan and Ognanović, Miloš and Đokić, Veljko R. and Prodanović, Radivoje and Todorović, Tamara", year = "2021", abstract = "Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to en-capsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein’s surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule, from −10.2 to −36.9 mV, as shown by zeta potential measurements and native PAGE electrophoresis. Biomineralization experiments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Periodate oxidation of carbohydrate components for glycoproteins can serve as a facile and general method for facilitating the biomimetic mineralization of other industrially relevant glycoproteins.", publisher = "MDPI", journal = "Polymers", title = "Chemical modification of glycoproteins’ carbohydrate moiety as a general strategy for the synthesis of efficient biocatalysts by biomimetic mineralization: The case of glucose oxidase", volume = "13", number = "22", pages = "2-12", doi = "10.3390/polym13223875" }
Stanišić, M. D., Popović Kokar, N., Ristić, P., Balaž, A. M., Senćanski, M., Ognanović, M., Đokić, V. R., Prodanović, R.,& Todorović, T.. (2021). Chemical modification of glycoproteins’ carbohydrate moiety as a general strategy for the synthesis of efficient biocatalysts by biomimetic mineralization: The case of glucose oxidase. in Polymers MDPI., 13(22), 2-12. https://doi.org/10.3390/polym13223875
Stanišić MD, Popović Kokar N, Ristić P, Balaž AM, Senćanski M, Ognanović M, Đokić VR, Prodanović R, Todorović T. Chemical modification of glycoproteins’ carbohydrate moiety as a general strategy for the synthesis of efficient biocatalysts by biomimetic mineralization: The case of glucose oxidase. in Polymers. 2021;13(22):2-12. doi:10.3390/polym13223875 .
Stanišić, Marija D., Popović Kokar, Nikolina, Ristić, Predrag, Balaž, Ana Marija, Senćanski, Milan, Ognanović, Miloš, Đokić, Veljko R., Prodanović, Radivoje, Todorović, Tamara, "Chemical modification of glycoproteins’ carbohydrate moiety as a general strategy for the synthesis of efficient biocatalysts by biomimetic mineralization: The case of glucose oxidase" in Polymers, 13, no. 22 (2021):2-12, https://doi.org/10.3390/polym13223875 . .